Degradation of organic sulfur compounds by a coal-solubilizing Fungus

Faison, B. D.; Clark, T. M.; Lewis, S. N.; Y., C.; Sharkey, D. M.; Woodward, Charlene A.

doi:10.1007/bf02922604

Cited by 33 publications

(11 citation statements)

References 15 publications

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“…The fungus Paecylomyces sp. carries out sulfur-specific oxidation of DBT by producing 2,2Ј-dihydroxybiphenyl (11). No yeasts have been reported to grow desulfurizing DBT and/or related thiophenic compounds that extensively occur in industrial fuel oils.…”

mentioning

confidence: 99%

Growth of Rhodosporidium toruloides Strain DBVPG 6662 on Dibenzothiophene Crystals and Orimulsion

Baldi

Pepi

Fava

2003

Appl Environ Microbiol

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Strains DBVPG 6662 and DBVPG 6739 of Rhodosporidium toruloides, a basidiomycete yeast, grew on thiosulfate as a sulfur source and glucose (2 g liter ؊1 or 10.75 mM) as a carbon source. DBVPG 6662 has a defective sulfate transport system, whereas DBVPG 6739 barely grew on sulfate. They were compared for the ability to use dibenzothiophene (DBT) and related organic sulfur compounds as sulfur sources. In the presence of glucose as a carbon source and DBT as a sulfur source, strain DBVPG 6662 grew better than DBVPG 6739. In the presence of thiosulfate as a sulfur source, the two yeast strains did not use DBT, DBT-sulfone, benzenesulfonic acid, biphenyl, and fluorene. When the two strains were grown in the presence of glucose, strain DBVPG 6662 transformed 27% of the DBT present (10 M) at a rate of 0.023 mol liter ؊1 h ؊1 in 36 h. Traces of 2,2-dihydroxylated biphenyl were transiently accumulated under these conditions. When the same strain was grown on glucose in the presence of a higher concentration of DBT (0.5 g liter ؊1 ), mainly in an insoluble form, the whole surface of the DBT crystals was colonized by a thick mycelium. This adherent structure was imaged by confocal microscopy with fluorescent concanavalin A, a lectin that specifically binds glucose and mannose residues. When DBVPG 6662 was grown on glucose in the presence of a commercial emulsion of bitumen, i.e., orimulsion, 68% of the benzo-and dibenzothiophenes and DBTs was removed after 15 days of incubation. The fungus adhered by hyphae to orimulsion droplets. When cultivated in the presence of commercial emulsifier-free fuel oil containing alkylated benzothiophenes and DBTs and having a composition similar to that of orimulsion, strain DBVPG 6662 removed only 11% of the total organic sulfur that occurs in the medium and did not adhere to the oil droplets. These results indicate that strain DBVPG 6662 is able to utilize the organic sulfur of DBT and a large variety of thiophenic compounds that occur extensively in commercial fuel oils by physically adhering to the organic sulfur source.

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confidence: 99%

Growth of Rhodosporidium toruloides Strain DBVPG 6662 on Dibenzothiophene Crystals and Orimulsion

Baldi

Pepi

Fava

2003

Appl Environ Microbiol

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“…However, the metabolism of disulfides is generally poorly understood and only a few studies with compounds such as diphenyl disulfide (16), dibenzyl disulfide (16), diallyl disulfide (20) and dimethyl disulfide (42) have identified sulfur-containing metabolites.…”

Section: Discussionmentioning

confidence: 99%

Aerobic Biodegradation of 2,2′-Dithiodibenzoic Acid Produced from Dibenzothiophene Metabolites

Young

Cheng

Fedorak

2006

Appl Environ Microbiol

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Dibenzothiophene is a sulfur heterocycle found in crude oils and coal. The biodegradation of dibenzothiophene through the Kodama pathway by Pseudomonas sp. strain BT1d leads to the formation of three disulfides: 2-oxo-2-(2-thiophenyl)ethanoic acid disulfide, 2-oxo-2-(2-thiophenyl)ethanoic acid-2-benzoic acid disulfide, and 2,2-dithiodibenzoic acid. When provided as the carbon and sulfur source in liquid medium, 2,2-dithiodibenzoic acid was degraded by soil enrichment cultures. Two bacterial isolates, designated strains RM1 and RM6, degraded 2,2-dithiodibenzoic acid when combined in the medium. Isolate RM6 was found to have an absolute requirement for vitamin B 12 , and it degraded 2,2-dithiodibenzoic acid in pure culture when the medium was supplemented with this vitamin. Isolate RM6 also degraded 2,2-dithiodibenzoic acid in medium containing sterilized supernatants from cultures of isolate RM1 grown on glucose or benzoate. Isolate RM6 was identified as a member of the genus Variovorax using the Biolog system and 16S rRNA gene analysis. Although the mechanism of disulfide metabolism could not be determined, benzoic acid was detected as a transient metabolite of 2,2-dithiodibenzoic acid biodegradation by Variovorax sp. strain RM6. In pure culture, this isolate mineralized 2,2-dithiodibenzoic acid, releasing 59% of the carbon as carbon dioxide and 88% of the sulfur as sulfate.

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“…It was able to degrade dibenzothiophene via the sulphur oxidation pathway leading to the formation of 2,2′-dihydroxybiphenyl. Apart from DBT, the fungal strain could also degrade ethyl phenyl sulphide and diphenyl sulphide resulting in the formation of corresponding sulphone [77]. Three distinct fungal strains, Trametes versicolor, Phanerochaeta chrysosporium and Pleurotus Sajor-Caju have been used to treat subbituminous coal high in sulphur from the Pirin Basin of Bulgaria.…”

Section: Fungal Strainsmentioning

confidence: 99%

Biodegradation of dibenzothiophene and its application in the production of clean coal

Mishra

Pradhan

Panda

et al. 2016

Fuel Processing Technology

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Degradation of organic sulfur compounds by a coal-solubilizing Fungus

Cited by 33 publications

References 15 publications

Growth of Rhodosporidium toruloides Strain DBVPG 6662 on Dibenzothiophene Crystals and Orimulsion

Growth of Rhodosporidium toruloides Strain DBVPG 6662 on Dibenzothiophene Crystals and Orimulsion

Aerobic Biodegradation of 2,2′-Dithiodibenzoic Acid Produced from Dibenzothiophene Metabolites

Biodegradation of dibenzothiophene and its application in the production of clean coal

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