Degradation of paraquat and diquat by the yeast Lipomyces starkeyi.

Shirata, Kazuto; Takagishi, Hidejiro

doi:10.2323/jgam.32.193

Cited by 11 publications

(4 citation statements)

References 6 publications

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“…attained 100% degradation of paraquat in a liquid culture after 3 days incubation; however, the contaminant concentration was only 27 ppm. When the paraquat concentration was increased two fold (54 ppm), biomass and paraquat degradation decreased notably to less than 10% (Hata et al 1986). The degradation obtained by H. dispersum ECS-705 (71%) is less than previously reported for P. putida.…”

Section: Discussionmentioning

confidence: 66%

Degradation of the herbicide paraquat by macromycetes isolated from southeastern Mexico

2017

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Fifty-four macromycetes, isolated from southeastern Mexico, were used in order to evaluate their capacity for degradation and tolerance to the herbicide paraquat. Ten of these strains were capable of growing in a solid culture medium in the presence of 200 ppm paraquat. Subsequently, assays to evaluate the degradation of the xenobiotic in a liquid medium were carried out. Of the ten strains evaluated, three presented the highest levels of degradation of the compound, which were (54.2%), (54.1%) and They presented the highest overall degradation percentage (70.7%) after 12 days culture. The presence of ligninolytic enzymes in these strains was evaluated. only presented aryl alcohol oxidase activity; however, with the data obtained, it was not possible to conclude whether this specific enzyme is responsible for paraquat degradation. The level of degradation obtained is above the one reported for , one of the few reports on paraquat degradation. This is the first report on the contaminant degradation capacity of.

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Section: Discussionmentioning

confidence: 66%

Degradation of the herbicide paraquat by macromycetes isolated from southeastern Mexico

2017

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“…The results from the present study revealed an overall decline in all the parameters as herbicide concentrations increased. The significance of herbicide concentration was underscored in a study by Hata et al (1986) where the yeast, Lipomyces sp. was able to totally eliminate (100 % degradation) paraquat dichloride from tainted broth at 27 ppm herbicide concentration, however, when the concentration was doubled to 54 ppm, degradation levels dropped below 10 %.…”

Section: Discussionmentioning

confidence: 99%

Effect of the chlorinated hydrocarbon herbicide, paraquat dichloride, on the growth properties and diversity of soil fungi

Osadebe

George

2022

MJEE

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This study explored acute herbicide-induced changes in soil fungal biomass, relative abundance, diversity and hyphal extension in response to five different application concentrations (1.625mg/kg -25.00mg/kg) of paraquat dichloride using the media dilution technique. There was a general decline in all the parameters investigated with increasing concentration and contact time. Of all the isolates obtained, Penicillium and Fusarium were the most frequently occurring amongst the filamentous fungi while Saccharomyces was the more frequently occurring yeast. Investigation of soil mycelial biomass revealed that the lowest dry weight was attained at the highest application rate of 25mg/kg representing a 61.71% reduction in mycelial biomass compared to the control. Based on the tolerance indices, the three isolates studied could be ranked in increasing order of tolerance as Mucor < Fusarium < Penicillium. Mucor showed no linear extension at concentrations of 3.25mg/kg and above while Penicillium exhibited linear extension even at 25mg/kg. At paraquat dichloride concentrations of 6.50mg/kg and over, growth inhibition levels for total soil fungi were over 50%; for Mucor, it was 100% at concentrations over 3.25mg/kg. Fusarium only exhibited 100% growth inhibition at the highest application concentration of 25mg/kg. Penicillium and total soil fungi were inhibited by 94% and 75% respectively at 25mg/kg. Statistically, soil fungal abundance and linear extension at herbicide concentrations of 3.25mg/kg and over were significantly different (at 95% confidence interval) from those obtained in the control experiments. It was concluded that the soil fungal community structure was radically altered by acute exposure to the herbicide, paraquat dichloride.

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“…Funderburk (1969) found that L. starkeyi was able to degrade both Diquat and paraquat. Furthermore, a bacterial consortium capable of achieving 97% paraquat (100 mg/L) degradation (Li et al, 2017), while Lipomyces yeast species were able to degrade 100% of available paraquat (27 mg/L) over a 3-day culture period in liquid medium (Hata et al, 1986). These prior studies concentrated on isolating and characterizing microbes that metabolized paraquat and Diquat.…”

Section: Introductionmentioning

confidence: 99%

RNA-sequencing analysis of the Diquat-degrading yeast strain Meyerozyma guilliermondii Wyslmt and the discovery of Diquat degrading genes

et al. 2022

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Diquat is used in agricultural contexts to control the growth of broadleaf and grassy weeds in both terrestrial and aquatic areas. Diquat can be readily absorbed by the soil and can remain therein for extended periods of time, altering the local microenvironment. In this study, the Meyerozyma guilliermondii Wyslmt yeast strain, which has the capacity to degrade Diquat, was isolated from soil exposed to long-term Diquat treatment. Over a 7-day incubation period, this strain was able to remove 42.51% of available Diquat (100 mg/L). RNA-Seq was performed to assess changes in gene expression in this yeast strain over the course of Diquat degradation, revealing 63 and 151 upregulated and downregulated genes, respectively. KEGG pathway enrichment analysis revealed these genes to be most highly enriched in the carbohydrate metabolism pathway. Through functional annotation and gene expression analyses, we identified seven genes were predicted to be involved in Diquat biodegradation. Results of qRT-PCR assays indicated that the relative mRNA expression levels of these seven genes were significantly higher relative to the control group. Together these analyses led to the identification of DN676 as a candidate Diquat-degrading gene. When a pET-DN676 vector was expressed in E. coli BL21, this strain was able to remove 12.49% of provided Diquat (100 mg/L) over the course of a 7-day incubation. These results thus confirmed that the DN676 gene can promote Diquat degradation, with these studies having yielded an engineered BL21-pET-DN676 bacterial strain capable of degrading Diquat.

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Degradation of paraquat and diquat by the yeast Lipomyces starkeyi.

Cited by 11 publications

References 6 publications

Degradation of the herbicide paraquat by macromycetes isolated from southeastern Mexico

Degradation of the herbicide paraquat by macromycetes isolated from southeastern Mexico

Effect of the chlorinated hydrocarbon herbicide, paraquat dichloride, on the growth properties and diversity of soil fungi

RNA-sequencing analysis of the Diquat-degrading yeast strain Meyerozyma guilliermondii Wyslmt and the discovery of Diquat degrading genes

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