Degradation of phenanthrene and naphthalene by a<i>Burkholderia</i>species strain

Kang, Hae Na; Hwang, Sang Yun; Kim, Y M; Kim, E; Kim, Y S; Kim, S K; Kim, S W; Cerniglia, Carl E.; Shuttleworth, Kay L.; Zylstra, Gerben J.

doi:10.1139/w03-009

Cited by 43 publications

(26 citation statements)

References 25 publications

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“…In addition, Burkholderia spp. TNFYE-5 and BS3702 and Burkholderia cepacia VUN 10001 were capable of degrading phenanthrene, and fluorene, phenanthrene, and pyrene (100 mg/l), respectively, as a sole carbon source 2,6,10) .…”

Section: Discussionmentioning

confidence: 99%

Change in the Predominant Bacteria in a Microbial Consortium Cultured on Media Containing Aromatic and Saturated Hydrocarbons as the Sole Carbon Source

2007

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A No.22 consortium was isolated from contaminated soils at oilfields by repeated transfer on a mineral salts medium with the aromatic hydrocarbon fraction from crude petroleum. The consortium was able to degrade aromatic hydrocarbons at high rates. Fourteen major DNA bands were detected in the consortium by PCR-DGGE. Three strains were capable of forming colonies on agarose plates containing mineral salts and aromatic hydrocarbons, and identified as Pandoraea sp. Y1, Hyphomicrobium facile Y3, and Burkholderia multivorans Y4 by 16S rRNA gene sequencing. The consortia of three cultures; an aromatic subculture, a saturated culture transferred from the subculture, and an aromatic obtained culture from the transferred culture, were compared using PCR-DGGE, a clone library, and quantitative real-time PCR. Burkholderia species containing strain Y4 became the dominant species in the saturated culture whereas its abundance decreased in the aromatic culture. Pandoraea sp. Y1, which was of low abundance in the saturated culture, and an unisolated bacterium, Brachymonas sp. F, in contrast, increased in the aromatic culture. The aromatic hydrocarbons were degraded by Pandoraea sp. Y1 and B. multivorans Y4, whereas the saturates were only degraded by B. multivorans Y4. The results indicate that the No.22 consortium adapts well to different carbon sources through a change in its predominant species. This study provides some basic information regarding bioremediation using the consortium.

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Section: Discussionmentioning

confidence: 99%

Change in the Predominant Bacteria in a Microbial Consortium Cultured on Media Containing Aromatic and Saturated Hydrocarbons as the Sole Carbon Source

2007

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“…Tech.,7 (4),[639][640][641][642][643][644][645][646][647][648][649][650][651][652] No. of cfu/mL bacteria and the metabolites formed during the degr adation was r epor ted by oth er wor ker s (Pinyakong et al, 2000;Moody et al, 2001;Kang et al, 2003;Kim et al, 2005). Wang et al (2008) reported phenanthrene degradation by microbial consortium under saline condition with phthalic acid as metabolite.…”

Section: Description Of Va3 Strain (Stenotrophomonas Maltophilia)mentioning

confidence: 99%

Biodegradation of polycyclic aromatic hydrocarbon by a halotolerant bacterial consortium isolated from marine environment

Arulazhagan

Vasudevan

Yeom

2010

Int. J. Environ. Sci. Technol.

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ABSTRACT:The biodegradability of polycyclic aromatic hydrocarbons such as naphthalene, fluorene, anthracene and phenanthrene by a halotolerant bacterial consortium isolated from marine environment was investigated. The polycyclic aromatic hydrocarbons degrading bacterial consortium was enriched from mixture saline water samples collected from Chennai (Port of Chennai, salt pan), India. The consortium potently degraded polycyclic aromatic hydrocarbons (> 95%) at 30g/L of sodium chloride concentration in 4 days. The consortium was able to degrade 39 to 45% of different polycyclic hydrocarbons at 60 g/L NaCl concentration. Due to increase in salinity, the percent degradation decreased. To enhance polycyclic aromatic hydrocarbons degradation, yeast extract was added as an additional substrate at 60g/L NaCl concentration. After the addition of yeast extract, the consortium degraded > 74 % of polycyclic aromatic hydrocarbons at 60 g/L NaCl concentration in 4 days. The consortium was also able to degrade PAHs at different concentrations (5, 10, 20, 50 and 100 ppm) with 30 g/L of NaCl concentration. The polycyclic aromatic hydrocarbons degrading halotolerant bacterial consortium consists of three bacterial strains, namely Ochrobactrum sp., Enterobacter cloacae and Stenotrophomonas maltophilia.

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“…For enzymes assays and metabolites determination thermophilic isolate was grown in mineral salt medium (MSM) [9] 4. Batch cultures were incubated in the dark at 60°C.…”

Section: Microorganism Isolation and Cultivationmentioning

confidence: 99%

“…Pathways by which mesophilic bacteria degrade aromatic hydrocarbons under aerobic conditions as well as enzymes involved in the metabolism of these hydrocarbons are well-studied [1][2][3][4]. On the other hand, little is known on the growth of thermophilic microorganisms on aromatic hydrocarbons, such as benzoic acid, cresols and phenols [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Degradation of naphthalene by thermophilic bacteria via a pathway, through protocatechuic acid

et al. 2008

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A number of thermophilic bacteria capable of utilizing naphthalene as a sole source of carbon were isolated from a high-temperature oilfield in Lithuania. These isolates were able to utilize several other aromatic compounds, such as anthracene, benzene, phenol, benzene-1, 3-diol, protocatechuic acid as well. Thermophilic isolate G27 ascribed to Geobacillus genus was found to have a high aromatic compound degrading capacity. Spectrophotometric determination of enzyme activities in cell-free extracts revealed that the last aromatic ring fission enzyme in naphthalene biotransformation by Geobacillus sp. G27 was inducible via protocatechuate 3, 4-dioxygenase; no protocatechuate 4, 5-dioxygenase, protocatechuate 2, 3-dioxygenase activities were detected. Intermediates such as o-phthalic and protocatechuic acids detected in culture supernatant confirmed that the metabolism of naphthalene by Geobacillus sp. G27 can proceed through protocatechuic acid via ortho-cleavage pathway and thus differs from the pathways known for mesophilic bacteria.

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Degradation of phenanthrene and naphthalene by aBurkholderiaspecies strain

Cited by 43 publications

References 25 publications

Change in the Predominant Bacteria in a Microbial Consortium Cultured on Media Containing Aromatic and Saturated Hydrocarbons as the Sole Carbon Source

Change in the Predominant Bacteria in a Microbial Consortium Cultured on Media Containing Aromatic and Saturated Hydrocarbons as the Sole Carbon Source

Biodegradation of polycyclic aromatic hydrocarbon by a halotolerant bacterial consortium isolated from marine environment

Degradation of naphthalene by thermophilic bacteria via a pathway, through protocatechuic acid

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