Degradation of phenol in wastewater in an upflow anaerobic sludge blanket reactor

Fang, Hhp; Chen, Tong; Li, Yu You; Chui, Ho Kwong

doi:10.1016/0043-1354(95)00309-6

Cited by 129 publications

(62 citation statements)

References 24 publications

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“…reported to be the predominant population in the mesophilic phenol-degrading granules (7,15). Although no other predominant morphotypes were observed in Fig.…”

Section: Sem Of Gac Sludgementioning

confidence: 84%

“…Consistent with the results from cloning, T-RFLP and FISH analyses suggested that members of the Methanosaeta and Syntrophorhabdaceae groups were the most predominant Archaea and Bacteria populations in the biofilm community. These thick filamentous Methanosaeta-like cells were randomly distributed in the biofilm sections, and have been found to be one of the predominant populations in phenol-degrading systems from laboratorial studies and an important population for the granulation of sludge (7,9,15). Some members of the Syntrophorhabdaceae have been suggested to be effective in the anaerobic degradation of organics such as terephthalate (44), isophthalate (29), trichlorobenzene (41), 1,2-dichloropropane (33) and phenol (9,16,17) in engineered ecosystems.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Active Microbes in a Full-Scale Anaerobic Fluidized Bed Reactor Treating Phenolic Wastewater

Chen

Tseng

et al. 2009

Microb. Environ.

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This study investigated the active microbial community in a full-scale granular activated carbon-anaerobic fluidized bed (GAC-AFB) reactor treating wastewater from the manufacturing of phenolic resin, using 16S rRNA-based molecular analyses. The results of cDNA from 16S rRNA revealed that Methanosaeta-related (83.9% of archaeal clones) and Syntrophorhabdaceae (formerly named Deltaproteobacteria group TA)-related (68.9% of bacterial clones) microorganisms were as the most predominant populations in the phenol-degrading GAC-AFB reactor. The high abundance of Syntrophorhabdaceae was supported by a terminal restriction fragment length polymorphism (T-RFLP) analysis, which showed that a Syntrophorhabdaceae-like fragment of 119 bp (~80% of total fragments) was the most predominant phylotype. Furthermore, fluorescence in situ hybridization (FISH) analyses suggested that Syntrophusand Chloroflexi-like cells were also in high abundance in the GAC biofilm. A non-layered structure of microorganisms was found in the GAC biofilm, where Methanosaeta (thick filamentous), Syntrophorhabdaceae (oval-shaped), Syntrophus (small rods) and Chloroflexi (thin-filamentous) were randomly distributed with high abundance. These findings greatly improve our understanding of the diversity and distribution of microbial populations in a full-scale mesophilic bioreactor treating an actual phenol-containing waste stream.Key words: phenol, anaerobic, microbial, phylogenetic, FISH Syntrophic association is an important process in anaerobic catabolism, whereby two or more microorganisms cooperate closely to completely degrade a substance neither can degrade alone. This process generally requires interaction between fermentative bacteria and methanogenic archaea (methanogens) to overcome the thermodynamic barrier in the initial step of degradation (e.g., fatty acids, solvents and aromatic substrates), and leads to the production of methane. Biodegradation of phenol under methanogenic conditions has been known for more than two decades (42, 48), and a number of different anaerobic processes (23,37,40,42) have been developed to successfully treat industrial waste streams containing phenol. A bottleneck to the starting up of these anaerobic processes was considered to be the cultivation/ acclimation of unique microbial specialists. A few reports have characterized these syntrophic phenol-degrading microorganisms. Fang and co-workers (16, 49) suggested that Desulfotomaculum, Clostridium and Syntrophus, together with Methanosaetaceae, Methanomicrobiales and Methanobacteriaceae, were the predominant microbial populations in a laboratory-scale upflow anaerobic sludge bed (UASB) reactor that degraded phenol under ambient temperature (26°C). Last year, we reported that Deltaproteobacteria group TA-and Pelotomaculum-related populations were the most important fermentative bacteria, in phenol-degrading enrichments under mesophilic (37°C) and thermophilic (55°C) conditions, respectively (9). More recently, a syntrophic phenol-degrading bacterium (Syntrophorhabd...

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“…reported to be the predominant population in the mesophilic phenol-degrading granules (7,15). Although no other predominant morphotypes were observed in Fig.…”

Section: Sem Of Gac Sludgementioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Characterization of Active Microbes in a Full-Scale Anaerobic Fluidized Bed Reactor Treating Phenolic Wastewater

Chen

Tseng

et al. 2009

Microb. Environ.

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“…Phenolic compounds were determined by gas chromatograph (Agilent Model: 6890 No. G1530, USA) according to the procedure given by Fang et al (1996). Volatile fatty acids (VFA) in the effluent were measured by the procedure outlined by Anushuya and Gupta (2006).…”

Section: Methodsmentioning

confidence: 99%

“…The filter zone in the hybrid reactor in addition to its physical role for biomass retention has some biological activity in contributing to COD reduction in a zone which is lacking in biomass in a classical UASB reactor (Tilche and Viera, 1991). Effluent recirculation has been an integral part of the treatment of phenolic wastewaters by UASB process (Fang et al, 1996;Zhou and Fang, 1997;Lay and Cheng, 1998;Fang and Zhou, 2000). The main objective of the present study was to find out the effect of recirculation of effluent for the treatment of complex phenolic coal wastewater employing hybrid UASB reactor.…”

Section: Introductionmentioning

confidence: 99%

Effect of effluent recycling and shock loading on the biodegradation of complex phenolic mixture in hybrid UASB reactors

Ramakrishnan

Gupta

2008

Bioresource Technology

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“…The critical factors affecting granulation are nutrient balance, pH [5,6], temperature [7], wastewater composition [8,9], EPS production and surface charge [10,11]. Since there is wide variety of wastewater providing different environmental and operational conditions in reactors due to different industries, it has become clear that some kinds of wastewaters are difficult to form granules and retain biomass successfully.…”

Section: Introductionmentioning

confidence: 99%

Release of Extracellular Polymeric Substance and Disintegration of Anaerobic Granular Sludge under Reduced Sulfur Compounds-Rich Conditions

Kobayashi

Chiku

2015

Energies

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Abstract:The effect of reduced form of sulfur compounds on granular sludge was investigated. Significant release of extracellular polymeric substance (EPS) from the granular sludge occurred in the presence of sulfide and methanethiol according to various concentrations. Granular sludge also showed a rapid increase in turbidity and decrease in diameter in accordance with sulfide concentration during the long-term shaking, suggesting that the strength of the granules was reduced with high-concentration sulfide. A continuous experiment of up-flow anaerobic sludge blanket reactors with different concentrations of sulfide (10, 200, 500 mg-S/L) influence demonstrated that the reactor fed with higher concentration of sulfide allowed more washout of small particle-suspended solid (SS) content and soluble carbohydrate and protein, which were considered as EPS released from biofilm. Finally, the presence of sulfide negatively affected methane production, chemical oxygen demand removal and sludge retention in operational performance.

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Degradation of phenol in wastewater in an upflow anaerobic sludge blanket reactor

Cited by 129 publications

References 24 publications

Characterization of Active Microbes in a Full-Scale Anaerobic Fluidized Bed Reactor Treating Phenolic Wastewater

Characterization of Active Microbes in a Full-Scale Anaerobic Fluidized Bed Reactor Treating Phenolic Wastewater

Effect of effluent recycling and shock loading on the biodegradation of complex phenolic mixture in hybrid UASB reactors

Release of Extracellular Polymeric Substance and Disintegration of Anaerobic Granular Sludge under Reduced Sulfur Compounds-Rich Conditions

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