Degradation of polycyclic aromatic hydrocarbons by the Chilean white-rot fungus Anthracophyllum discolor

Acevedo, Francisca; Pizzul, Leticia; Castillo, María del Pilar; Cuevas, Raphael; Díez, M.C.

doi:10.1016/j.jhazmat.2010.09.020

Cited by 161 publications

(43 citation statements)

References 58 publications

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“…4 Plate-based enzymatic screening of T. asperellum H15. a ABTS oxidation after 2 days of growth in minimal medium; b o-anisidine oxidation after 6 days of growth in modified Kirk medium; c azure B decoloration after 10 days of growth in Czapeck medium (Acevedo et al 2011). The same was observed with strains of Fusarium sp., Monilinia sp., Aspergillus terreus and Talaromyces spectabilis strains in soil microcosms and solid culture when using similar LMW and HMW-PAH mixtures, even in the presence of concentrations below 100 mg Kg −1 of individual PAHs (Reyes-Cesar et al 2014;Thion et al 2013;Wu et al 2008;) and regarding the degradation of 10 mg Kg −1 of Pyr in liquid culture by Trichoderma harzianum (Ravelet et al 2000).…”

Section: Discussionsupporting

confidence: 55%

Degradation of polycyclic aromatic hydrocarbons in soil by a tolerant strain of Trichoderma asperellum

Zafra

Moreno-Montaño

Absalón

et al. 2014

Environ Sci Pollut Res

100

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Trichoderma asperellum H15, a previously isolated strain characterized by its high tolerance to low (LMW) and high molecular weight (HMW) PAHs, was tested for its ability to degrade 3-5 ring PAHs (phenanthrene, pyrene, and benzo[a]pyrene) in soil microcosms along with a biostimulation treatment with sugarcane bagasse. T. asperellum H15 rapidly adapted to PAH-contaminated soils, producing more CO2 than uncontaminated microcosms and achieving up to 78 % of phenanthrene degradation in soils contaminated with 1,000 mg Kg(-1) after 14 days. In soils contaminated with 1,000 mg Kg(-1) of a three-PAH mixture, strain H15 was shown to degrade 74 % phenanthrene, 63 % pyrene, and 81 % of benzo[a]pyrene. Fungal catechol 1,2 dioxygenase, laccase, and peroxidase enzyme activities were found to be involved in the degradation of PAHs by T. asperellum. The results demonstrated the potential of T. asperellum H15 to be used in a bioremediation process. This is the first report describing the involvement of T. asperellum in LMW and HMW-PAH degradation in soils. These findings, along with the ability to remove large amounts of PAHs in soil found in the present work provide enough evidence to consider T. asperellum as a promising and efficient PAH-degrading microorganism.

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Section: Discussionsupporting

confidence: 55%

Degradation of polycyclic aromatic hydrocarbons in soil by a tolerant strain of Trichoderma asperellum

Zafra

Moreno-Montaño

Absalón

et al. 2014

Environ Sci Pollut Res

100

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“…Similar to other low molecular weight (LMW) PAHs such as naphthalene and phenanthrene, anthracene can be degraded by a wide variety of microorganisms. Isolated bacteria capable of anthracene metabolism include organisms from both Gram-positive (Dean-Ross et al, 2001;Khan et al, 2002;Zeinali et al, 2007;Zhang et al, 2009;Zeng et al, 2010;Ling et al, 2011) and Gram-negative genera (Story et al, 2004;Jacques et al, 2005;Baboshin et al, 2008;Arulazhagan and Vasudevan, 2011;Jin et al, 2012), and several fungi have been described that are capable of transforming anthracene (Wu et al, 2010;Acevedo et al, 2011). However, only recently have culture-independent methods been applied to directly link uncultivated or uncharacterized bacteria to the metabolism of anthracene in contaminated environments.…”

Section: Introductionmentioning

confidence: 99%

Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil

Singleton²,

Aitken³

2013

Environmental Engineering Science

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Stable-isotope probing was previously used to identify bacterial anthracene-degraders in untreated soil from a former manufactured gas plant site. However, subsequent pyrosequence analyses of total bacterial communities and quantification of 16S rRNA genes indicated that relative abundances of the predominant anthracene-degrading bacteria (designated Anthracene Group 1) diminished as a result of biological treatment conditions in lab-scale, aerobic bioreactors. This study identified Alphaproteobacterial anthracene-degrading bacteria in bioreactor-treated soil which were dissimilar to those previously identified. The largest group of sequences was from the Alterythrobacter genus while other groups of sequences were associated with bacteria within the order Rhizobiales and the genus Bradyrhizobium. Conditions in the bioreactor enriched for organisms capable of degrading anthracene which were not the same as those identified as dominant degraders in the untreated soil. Further, these data suggest that identification of polycyclic aromatic hydrocarbon-degrading bacteria in contaminated but untreated soil may be a poor indicator of the most active degraders during biological treatment.

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“…Therefore, it is necessary to assess the influence of mixtures of PAHs on their biodegradation. These influences include no significant impact; negative effects, including inhibition; and positive effects, including cometabolism (Beckles et al 1998;Sang et al 2009;Acevedo et al 2011). Our data indicates that the presence of BaA did not significantly influence the fungal degradation of Ace in sandy soil and vice versa.…”

Section: Influence Of Mixtures Of Acenaphthylene and Benzo[a] Anthracmentioning

confidence: 59%

Influence of mixtures of acenaphthylene and benzo[a]anthracene on their degradation by Pleurotus ostreatus in sandy soil

et al. 2013

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Purpose Polycyclic aromatic hydrocarbons (PAHs) are a class of organic compounds commonly found as soil contaminants. Fungal degradation is considered as an environmentally friendly and cost-effective approach to remove PAHs from soil. Acenaphthylene (Ace) and Benzo[a]anthracene (BaA) are two PAHs that can coexist in soils; however, the influence of the presence of each other on their biodegradation has not been studied. The biodegradation of Ace and BaA, alone and in mixtures, by the white rot fungus Pleurotus ostreatus was studied in a sandy soil. Materials and methods Experimental microcosms containing soil spiked with different concentrations of Ace and BaA were inoculated with P. ostreatus. Initial (t 0 ) and final (after 15 days of incubation) soil concentrations of Ace and BaA were determined after extraction of the PAHs.Results and discussion P. ostreatus was able to degrade 57.7% of the Ace in soil spiked at 30 mg kg −1 dry soil and 65.8% of Ace in soil spiked at 60 mg kg −1 dry soil. The degradation efficiency of BaA by P. ostreatus was 86.7 and 77.4% in soil spiked with Ace at 30 and 60 mg kg −1 dry soil, respectively. After 15 days of incubation, there were no significant differences in Ace concentration between soil spiked with Ace and soil spiked with Ace + BaA, irrespective of the initial soil concentration of both PAHs. There were also no differences in BaA concentration between soil spiked with BaA and soil spiked with BaA + Ace. Conclusions The results indicate that the fungal degradation of Ace and BaA was not influenced by the presence of each other's PAH in sandy soil. Bioremediation of soils contaminated with Ace and BaA using P. ostreatus is a promising approach to eliminate these PAHs from the environment.

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Degradation of polycyclic aromatic hydrocarbons by the Chilean white-rot fungus Anthracophyllum discolor

Cited by 161 publications

References 58 publications

Degradation of polycyclic aromatic hydrocarbons in soil by a tolerant strain of Trichoderma asperellum

Degradation of polycyclic aromatic hydrocarbons in soil by a tolerant strain of Trichoderma asperellum

Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil

Influence of mixtures of acenaphthylene and benzo[a]anthracene on their degradation by Pleurotus ostreatus in sandy soil

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