Degradation of polyethylene by Klebsiella pneumoniae Mk-1 isolated from soil

Zhang, Xian; Feng, Xu; Yuan, Lin; Gou, Hongmei; Zhang, Yao; Yang, Lijuan

doi:10.1016/j.ecoenv.2023.114965

Cited by 16 publications

(4 citation statements)

References 21 publications

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“…molitor. ,, A bacterium belonging to Klebsiella sp. (Klebsiella pneumoniae Mk-1) has been isolated from soil and confirmed to degrade PE …”

Section: Resultsmentioning

confidence: 99%

Molecular-Weight-Dependent Degradation of Plastics: Deciphering Host–Microbiome Synergy Biodegradation of High-Purity Polypropylene Microplastics by Mealworms

He,

Ding,

Yang

et al. 2024

Environ. Sci. Technol.

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The biodegradation of polypropylene (PP), a highly persistent nonhydrolyzable polymer, by Tenebrio molitor has been confirmed using commercial PP microplastics (MPs) (M n 26.59 and M w 187.12 kDa). This confirmation was based on the reduction of the PP mass, change in molecular weight (MW), and a positive Δδ 13 C in the residual PP. A MW-dependent biodegradation mechanism was investigated using five high-purity PP MPs, classified into low (0.83 and 6.20 kDa), medium (50.40 and 108.0 kDa), and high (575.0 kDa) MW categories to access the impact of MW on the depolymerization pattern and associated gene expression of gut bacteria and the larval host. The larvae can depolymerize/biodegrade PP polymers with high MW although the consumption rate and weight losses increased, and survival rates declined with increasing PP MW. This pattern is similar to observations with polystyrene (PS) and polyethylene (PE), i.e., both M n and M w decreased after being fed low MW PP, while M n and/or M w increased after high MW PP was fed. The gut microbiota exhibited specific bacteria associations, such as Kluyvera sp. and Pediococcus sp. for high MW PP degradation, Acinetobacter sp. for medium MW PP, and Bacillus sp. alongside three other bacteria for low MW PP metabolism. In the host transcriptome, digestive enzymes and plastic degradation-related bacterial enzymes were up-regulated after feeding on PP depending on different MWs. The T. molitor host exhibited both defensive function and degradation capability during the biodegradation of plastics, with high MW PP showing a relatively negative impact on the larvae.

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“…molitor. ,, A bacterium belonging to Klebsiella sp. (Klebsiella pneumoniae Mk-1) has been isolated from soil and confirmed to degrade PE …”

Section: Resultsmentioning

confidence: 99%

Molecular-Weight-Dependent Degradation of Plastics: Deciphering Host–Microbiome Synergy Biodegradation of High-Purity Polypropylene Microplastics by Mealworms

He,

Ding,

Yang

et al. 2024

Environ. Sci. Technol.

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“…The L. plantarum LPZN19 MCO contains two served regions with unique copper-binding sites, which are typical structural featur MCOs [33]. Correspondingly, L. plantarum LPZN19 MCO was predicted to be 56.85 Predicted molecular weights of MCO have been reported in Acinetobacter baumanni (72 KDa), Klebsiella pneumoniae [35] (60 KDa), Pseudomonas sp. [15] (52 KDa), Klebsiel [22] (58.2 KDa) and Ochrobactrum sp.…”

Section: Discussionmentioning

confidence: 99%

“…Correspondingly, L. plantarum LPZN19 MCO was predicted to be 56.85 kDa. Predicted molecular weights of MCO have been reported in Acinetobacter baumannii [34] (72 KDa), Klebsiella pneumoniae [35] (60 KDa), Pseudomonas sp. [15] (52 KDa), Klebsiella sp.…”

Section: Discussionmentioning

confidence: 99%

Research of Multicopper Oxidase and Its Degradation of Histamine in Lactiplantibacillus plantarum LPZN19

Pei,

Wang,

et al. 2023

Microorganisms

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In order to explore the structural changes and products of histamine degradation by multicopper oxidase (MCO) in Lactiplantibacillus plantarum LPZN19, a 1500 bp MCO gene in L. plantarum LPZN19 was cloned, and the recombinant MCO was expressed in E. coli BL21 (DE3). After purification by Ni2+-NTA affinity chromatography, the obtained MCO has a molecular weight of 58 kDa, and it also has the highest enzyme activity at 50 °C and pH 3.5, with a relative enzyme activity of 100%, and it maintains 57.71% of the relative enzyme activity at 5% salt concentration. The secondary structure of MCO was determined by circular dichroism, in which the proportions of the α-helix, β-sheet, β-turn and random coil were 2.9%, 39.7%, 21.2% and 36.1%, respectively. The 6xj0.1.A with a credibility of 68.21% was selected as the template to predict the tertiary structure of MCO in L. plantarum LPZN19, and the results indicated that the main components of the tertiary structure of MCO were formed by the further coiling and folding of a random coil and β-sheet. Histamine could change the spatial structure of MCO by increasing the content of the α-helix and β-sheet. Finally, the LC-MS/MS identification results suggest that the histamine was degraded into imidazole acetaldehyde, hydrogen peroxide and ammonia.

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“…Presumably, PE undergoes mechanical and microbial breakdown, leading to shorter chains and reduced molecular weight. PET is cleaved by gut microorganisms or hydrolysis at ester linkages, resulting in a reduction in molecular weight. , In addition, compared to larvae fed on PE and PET without added antibiotics, significant changes were observed in the dominant microbial populations in the larval guts in the presence of antibiotics (Figure a), particularly with higher relative abundances of antibiotic-resistant and plastic-degrading bacteria Klebsiella , Lelliottia , and Enterobacter that enhanced a decrease in the average molecular weight of feces. This result demonstrates that changes in the microbiome composition of the larval gut and the enrichment of antibiotic-resistant and plastic-degrading bacteria impacted the depolymerization and degradation of PE and PET MPs.…”

Section: Discussionmentioning

confidence: 99%

Understanding the Ecological Robustness and Adaptability of the Gut Microbiome in Plastic-Degrading Superworms (Zophobas atratus) in Response to Microplastics and Antibiotics

Lu,

Lou,

Wang

et al. 2024

Environ. Sci. Technol.

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Recent discoveries indicate that several insect larvae are capable of ingesting and biodegrading plastics rapidly and symbiotically, but the ecological adaptability of the larval gut microbiome to microplastics (MPs) remains unclear. Here, we described the gut microbiome assemblage and MP biodegradation of superworms (Zophobas atratus larvae) fed MPs of five major petroleum-based polymers (polyethylene, polypropylene, polystyrene, polyvinyl chloride, and polyethylene terephthalate) and antibiotics. The shift of molecular weight distribution, characteristic peaks of C�O, and metabolic intermediates of residual polymers in egested frass proved depolymerization and biodegradation of all MPs tested in the larval intestines, even under antibiotic suppression. Superworms showed a wide adaptation to the digestion of the five polymer MPs. Antibiotic suppression negatively influenced the survival rate and plastic depolymerization patterns. The larval gut microbiomes differed from those fed MPs and antibiotics, indicating that antibiotic supplementation substantially shaped the gut microbiome composition. The larval gut microbiomes fed MPs had higher network complexity and stability than those fed MPs and antibiotics, suggesting that the ecological robustness of the gut microbiomes ensured the functional adaptability of larvae to different MPs. In addition, Mantel's test indicated that the gut microbiome assemblage was obviously related to the polymer type, the plastic degradability, antibiotic stress, and larval survival rate. This finding provided novel insights into the self-adaptation of the gut microbiome of superworms in response to different MPs.

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Degradation of polyethylene by Klebsiella pneumoniae Mk-1 isolated from soil

Cited by 16 publications

References 21 publications

Molecular-Weight-Dependent Degradation of Plastics: Deciphering Host–Microbiome Synergy Biodegradation of High-Purity Polypropylene Microplastics by Mealworms

Molecular-Weight-Dependent Degradation of Plastics: Deciphering Host–Microbiome Synergy Biodegradation of High-Purity Polypropylene Microplastics by Mealworms

Research of Multicopper Oxidase and Its Degradation of Histamine in Lactiplantibacillus plantarum LPZN19

Understanding the Ecological Robustness and Adaptability of the Gut Microbiome in Plastic-Degrading Superworms (Zophobas atratus) in Response to Microplastics and Antibiotics

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