Degradation of soil-applied isomers of hexachlorocyclohexane by a Pseudomonas SP

Sahu, Suprava; Patnaik, K. K.; Bhuyan, Sasmita; Sethunathan, N.

doi:10.1016/0038-0717(93)90139-3

Cited by 39 publications

(35 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pseudomonas sp has been reported to degrade other isomers of HCH in soil but not β-HCH [19]. But in the present study when SMS from P. florida cultivation was amended with soil degradation of persistent isomers like α-, β-and δ-HCH was accelerated in addition to the less persistent γ-HCH.…”

Section: Discussioncontrasting

confidence: 63%

See 1 more Smart Citation

Biodegradation of Hexachlorocyclohexane (HCH) Isomers by White Rot Fungus, Pleurotus florida

Mohapatra¹

2015

J Bioremed Biodeg

View full text Add to dashboard Cite

Hexachlorocyclohexane (HCH) isomers are reported to persist in the environment long after their usage is discontinued. Pleurotus florida, a white rot fungus, was found to degrade not only γ-HCH but also its more persistent isomers individually as well as a mixture in a Raper's complete medium. Within 30 days γ-HCH degraded completely, whereas other 3 isomers degraded 92-99%. The degradation rate was γ-HCH>β-HCH>α-and δ-HCH. The mycelium biomass was free from γ-HCH residues but accumulated about 3% residues of other 3 isomers. Presence of intermediate metabolites was not detected indicating complete mineralization of HCH isomers. Ability of P. florida to degrade HCH isomers was further studied in soil by amendment with spent mushroom substrate (SMS). SMS addition could marginally increase degradation of α-, β-and δ-HCH, but significantly increased degradation of γ-HCH. When the study was repeated similar trend was observed. The half-life of degradation of γ-HCH was 439-570 days in un-amended soil while 37-42 days in SMS amended soil. For other 3 stable isomers the half-life was reduced from 686-828 to 88-125 days by SMS amendments. These results indicate that SMS from P. florida cultivation can be utilized for bioremediation of HCH contaminated site.

show abstract

Section: Discussioncontrasting

confidence: 63%

“…A Pseudomonas sp could readily degrade α-, β-and γ-HCH in a medium but degraded α-and γ-HCH in soil, but not β-HCH [19]. Biodegradation of HCH isomers in contaminated soils has been carried out using an isolate Pseudomonas aeruginosa ITRC-5.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation of Hexachlorocyclohexane (HCH) Isomers by White Rot Fungus, Pleurotus florida

Mohapatra¹

2015

J Bioremed Biodeg

View full text Add to dashboard Cite

show abstract

“…Earlier studies suggested that degradation of HCH was faster under anoxic conditions and that microbial degradation was primary route of HCH disappearance from soil (MacRae et al, 1967). Microbial degradation of all the HCH-isomers has since been observed under oxic conditions both in soil (Bachmann et al, 1988 b); Doelman et al, 1985;Sahu et al, 1993) and in pure cultures of microorganisms Thomas et al, 1996). We have isolated in our laboratory a microbial consortium consisting of ten bacterial isolates which have got the capacity to degrade HCH (Manonmani et al, 2000;Murthy and Manonmani, 2007) under oxic conditions.…”

Section: Discussionmentioning

confidence: 96%

“…Degradation of HCH was faster under anoxic conditions and that microbial degradation was primary route of HCH disappearance from soil (MacRae et al, 1967). Microbial degradation of all the HCH-isomers has since been observed under oxic conditions both in soil (Bachmann et al, 1988 (a,b); Doelman et al, 1985;Sahu et al, 1993) and in pure cultures of microorganisms Thomas et al, 1996). Soil slurry has been adopted for the microbial degradation of pesticides, explosives, polynuclear aromatic hydrocarbons, and chlorinated organic pollutants (Gonzalez et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Bioremediation of Hexachlorocyclohexane Contaminated Soil: Field Trials

Manonmani¹

2011

Pesticides in the Modern World - Pesticides Use and Management

View full text Add to dashboard Cite

“…Viable counts were made by plating suitably diluted soil suspensions on nutrient agar medium. The plates were incubated at 30 °C for 72 h and the colonies of individual members of the consortium were counted (Sahu et al, 1996).…”

Section: Determination Of Growthmentioning

confidence: 99%

Remediation of Chlorpyrifos in Artificially Spiked Soil by Defined Microbial Consortium

Srinivas¹,

Prasad²,

Krishnan³

2016

IJSRES

View full text Add to dashboard Cite

Abstract. Excessive use of pesticides as plant protectants has led to the contamination of ecosystem in all parts of the world. This widespread use of pesticides for agricultural purposes has resulted in the persistence of their residues in various environmental matrices, such as soil, water and air. To remove these residues from the environment bioremediation has been proven a suitable technique for reducing pesticide contamination as microorganisms are capable of mineralizing them completely. Chlorpyrifos is a broad spectrum moderately toxic organophosphorous insecticide. It is widely used in agriculture for pest control and in households as a termicide. Long term exposure to chlorpyrifos residues and its metabolites can affect the human health. A microbial consortium that can utilize Chlorpyrifos as a sole source of carbon and energy was isolated from soil through a novel technique involving an initial enrichment in a column reactor followed by enrichment in a shake flask. A diverse range of microorganisms responsible for Chlorpyrifos degradation has been developed. In the present investigation, a new microbial consortium degrading Quinolphos has been developed. Factors such induction, inoculum level, concentration of the substrate, soil pH, soil moisture etc affecting degradation were also studied. Inoculum level of 500µg protein/mL, and a pH 7.5 at ambient temperature (26-28 •C) resulted in degradation of Chlorpyrifos upto 50ppm.

show abstract

Degradation of soil-applied isomers of hexachlorocyclohexane by a Pseudomonas SP

Cited by 39 publications

References 20 publications

Biodegradation of Hexachlorocyclohexane (HCH) Isomers by White Rot Fungus, Pleurotus florida

Biodegradation of Hexachlorocyclohexane (HCH) Isomers by White Rot Fungus, Pleurotus florida

Bioremediation of Hexachlorocyclohexane Contaminated Soil: Field Trials

Remediation of Chlorpyrifos in Artificially Spiked Soil by Defined Microbial Consortium

Contact Info

Product

Resources

About