Degradation of the Endoplasmic Reticulum by Autophagy during Endoplasmic Reticulum Stress in <i>Arabidopsis</i>

Liu, Yimo; Burgos, Junmarie Soto; Deng, Yan; Srivastava, Renu; Howell, Stephen H.; Bassham, Diane C.

doi:10.1105/tpc.112.101535

Cited by 244 publications

(279 citation statements)

References 98 publications

(157 reference statements)

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“…When plant cells undergo ER stress, a punctate pattern of the ER marker GFP-HDEL was found in the vacuole of leaf protoplasts treated with CA (Liu et al, 2012). Some of the GFP-HDEL puncta were colocalized with an autophagic marker, Cerulean-ATG8e, but others were not (Liu et al, 2012), similar to our data ( Figures 4C and 4H). Likewise, a small fraction of Rubisco-containing bodies lacking GFP-ATG8 signals were evident when mesophyll cells were treated with CA (Ishida et al, 2008).…”

Section: Discussionsupporting

confidence: 89%

“…We compared our colocalization images with similar data that other research groups recently published to investigate the autophagy of plant organelles (Ishida et al, 2008;Liu et al, 2012). When plant cells undergo ER stress, a punctate pattern of the ER marker GFP-HDEL was found in the vacuole of leaf protoplasts treated with CA (Liu et al, 2012). Some of the GFP-HDEL puncta were colocalized with an autophagic marker, Cerulean-ATG8e, but others were not (Liu et al, 2012), similar to our data ( Figures 4C and 4H).…”

Section: Discussionsupporting

confidence: 85%

“…It appears that plant organelles delivered to the vacuole by autophagy either contain or lack ATG8 markers. We compared our colocalization images with similar data that other research groups recently published to investigate the autophagy of plant organelles (Ishida et al, 2008;Liu et al, 2012). When plant cells undergo ER stress, a punctate pattern of the ER marker GFP-HDEL was found in the vacuole of leaf protoplasts treated with CA (Liu et al, 2012).…”

Section: Discussionmentioning

confidence: 76%

“…ATG5 and other core ATG genes are involved in the degradation of chloroplasts (Ishida et al, 2008;Wada et al, 2009), amyloplasts (Nakayama et al, 2012), and endoplasmic reticulum (ER) (Liu et al, 2012). However, whether the core ATG genes are also required for the degradation of other types of organelles, such as peroxisomes, mitochondria, and oil bodies, has not been clear.…”

Section: Discussionmentioning

confidence: 99%

“…CA inhibits degradation in the vacuole by blocking the acidification of the vacuole and has been used to stabilize autophagic cargoes in the vacuole (Yoshimoto et al, 2004;Wada et al, 2009;Liu et al, 2012). If peroxisomes are autophagic cargoes targeted into the vacuole for degradation, CA may stabilize peroxisomes that would be degraded otherwise.…”

Section: Peroxisomal Proteins Are Targeted To the Central Vacuole Formentioning

confidence: 99%

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Autophagy-Related Proteins Are Required for Degradation of Peroxisomes inArabidopsisHypocotyls during Seedling Growth

et al. 2013

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Plant peroxisomes play a pivotal role during postgerminative growth by breaking down fatty acids to provide fixed carbons for seedlings before the onset of photosynthesis. The enzyme composition of peroxisomes changes during the transition of the seedling from a heterotrophic to an autotrophic state; however, the mechanisms for the degradation of obsolete peroxisomal proteins remain elusive. One candidate mechanism is autophagy, a bulk degradation pathway targeting cytoplasmic constituents to the lytic vacuole. We present evidence supporting the autophagy of peroxisomes in Arabidopsis thaliana hypocotyls during seedling growth. Mutants defective in autophagy appeared to accumulate excess peroxisomes in hypocotyl cells. When degradation in the vacuole was pharmacologically compromised, both autophagic bodies and peroxisomal markers were detected in the wild-type vacuole but not in that of the autophagy-incompetent mutants. On the basis of the genetic and cell biological data we obtained, we propose that autophagy is important for the maintenance of peroxisome number and cell remodeling in Arabidopsis hypocotyls.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Peroxisomal Proteins Are Targeted To the Central Vacuole Formentioning

confidence: 99%

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Autophagy-Related Proteins Are Required for Degradation of Peroxisomes inArabidopsisHypocotyls during Seedling Growth

et al. 2013

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Metabolism and autophagy in plants—a perfect match

et al. 2022

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Autophagy is a eukaryotic cellular transport mechanism that delivers intracellular macromolecules, proteins, and even organelles to a lytic organelle (vacuole in yeast and plants/lysosome in animals) for degradation and nutrient recycling. The process is mediated by highly conserved autophagy-related (ATG) proteins. In plants, autophagy maintains cellular homeostasis under favorable conditions, guaranteeing normal plant growth and fitness. Severe stress such as nutrient starvation and plant senescence further induce it, thus ensuring plant survival under unfavorable conditions by providing nutrients through the removal of damaged or aged proteins, or organelles. In this article, we examine the interplay between metabolism and autophagy, focusing on the different aspects of this reciprocal relationship. We show that autophagy has a strong influence on a range of metabolic processes, whereas at the same time, even single metabolites can activate autophagy. We highlight the involvement of ATG genes in metabolism, examine the role of the macronutrients carbon and nitrogen, and various micronutrients, and take a closer look at how the interaction between autophagy and metabolism impacts on plant phenotypes and yield.

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Cargo receptors and adaptors for selective autophagy in plant cells

et al. 2022

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Plant selective (macro)autophagy is a highly regulated process where eukaryotic cells spatiotemporally degrade some of their constituents that have become superfluous or harmful. The identification and characterization of the factors determining this selectivity make it possible to integrate selective (macro)autophagy into plant cell physiology and homeostasis. The specific cargo receptors and/or scaffold proteins involved in this pathway are generally not structurally conserved, as are the biochemical mechanisms underlying recognition and integration of a given cargo into the autophagosome in different cell types. This review discusses the few specific cargo receptors described in plant cells to highlight key features of selective autophagy in the plant kingdom and its integration with plant physiology, aiming to identify evolutionary convergence and knowledge gaps to be filled by future research.

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Degradation of the Endoplasmic Reticulum by Autophagy during Endoplasmic Reticulum Stress in Arabidopsis

Cited by 244 publications

References 98 publications

Autophagy-Related Proteins Are Required for Degradation of Peroxisomes inArabidopsisHypocotyls during Seedling Growth

Autophagy-Related Proteins Are Required for Degradation of Peroxisomes inArabidopsisHypocotyls during Seedling Growth

Metabolism and autophagy in plants—a perfect match

Cargo receptors and adaptors for selective autophagy in plant cells

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