Degradation of the phenolic β-ether lignin model dimer and dyes by dye-decolorizing peroxidase from Bacillus amyloliquefaciens

Yang, Juan; Gao, Tongguo; Zhang, Yaru; Wang, Shuo; Hong-ya, LI; Li, Shuna; ShuXiang, Wang

doi:10.1007/s10529-019-02696-0

Cited by 31 publications

(15 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chemical structure of GGE is the major structural unit in Kraft lignin, and there are several possible sites for oxidation or oxidative cleavage (Yang et al, 2019). In this study, the detection of the product with a molecular weight of 495 m/z indicated that the F43Y/T67R Mb can indeed degrade GGE.…”

Section: Oxidation Products Of Gge Catalyzed By F43y/t67r Mbmentioning

confidence: 56%

Biotransformation of Lignin by an Artificial Heme Enzyme Designed in Myoglobin With a Covalently Linked Heme Group

Guo

Liu

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The conversion of Kraft lignin in plant biomass into renewable chemicals, aiming at harvesting aromatic compounds, is a challenge process in biorefinery. Comparing to the traditional chemical methods, enzymatic catalysis provides a gentle way for the degradation of lignin. Alternative to natural enzymes, artificial enzymes have been received much attention for potential applications. We herein achieved the biodegradation of Kraft lignin using an artificial peroxidase rationally designed in myoglobin (Mb), F43Y/T67R Mb, with a covalently linked heme cofactor. The artificial enzyme of F43Y/T67R Mb has improved catalytic efficiencies at mild acidic pH for phenolic and aromatic amine substrates, including Kraft lignin and the model lignin dimer guaiacylglycerol-β-guaiacyl ether (GGE). We proposed a possible catalytic mechanism for the biotransformation of lignin catalyzed by the enzyme, based on the results of kinetic UV-Vis studies and UPLC-ESI-MS analysis, as well as molecular modeling studies. With the advantages of F43Y/T67R Mb, such as the high-yield by overexpression in E. coli cells and the enhanced protein stability, this study suggests that the artificial enzyme has potential applications in the biodegradation of lignin to provide sustainable bioresource.

show abstract

Section: Oxidation Products Of Gge Catalyzed By F43y/t67r Mbmentioning

confidence: 56%

Biotransformation of Lignin by an Artificial Heme Enzyme Designed in Myoglobin With a Covalently Linked Heme Group

Guo

Liu

et al. 2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…In the present study, lignin content was negatively related to POX activity. Such relation indicated that this enzyme was not involved in lignin synthesis and/or exhibited high oxidative activity in the degradation of lignin dimer [73]. Moreover, the increase in peroxidase activity might be due to the accumulation of certain phenylpropanoids (monomeric units of lignin) resulting in the reduction of structural lignin [74].…”

Section: Resultsmentioning

confidence: 99%

Thymelaea hirsuta and Echinops spinosus: Xerophytic Plants with High Potential for First-Generation Biodiesel Production

et al. 2020

View full text Add to dashboard Cite

The negative impacts of fossil fuel on the environment should be replaced by clean and sustainable energy sources worldwide. Therefore, the use of biodiesel as a clean energy source is crucial. Biodiesel is produced from various natural resources through a transesterification process. Considering the importance of this topic, this study focuses on the assessment of oil properties of Thymelaea hirsuta and Echinops spinosus as primary sources for biodiesel production. The two investigated plants were collected from the Western Desert of Egypt. The results showed that the lignocellulosic content was about 57.3 and 79.8 g/100 g in E. spinosus and 59.1 and 82.8 g/100 g in T. hirsuta, respectively. The two investigated samples showed variable lipid contents (30.2–76.1%). The GC-MS fatty acid profile characterized seven FAs in E. spinosus and twelf FAs in T. hirsuta. The greatest CN was calculated in T. hirsuta (379.2) compared to the lowest in E. spinosus (229.9). Furthermore, the values of saponification number (SN) were 27.9 in E. spinosus and 16.07 in T. hirsuta. The value of higher heating value (HHV) was about 47.5 MJ/kg in E. spinosus and 48.3 MJ/kg in T. hirsuta. Meanwhile, T. hirsuta exhibited a higher induction period (IP) value (19.3 h) comparable to that of E. spinosus (4.3 h). The results revealed that both plants are potential sources for biodiesel production according to various international standards for biodiesel production, and this work appears to be one of the first reports regarding such wild xerophytic plants as promising new primary sources for biodiesel production in Egypt.

show abstract

“…DypB was characterized from a soil bacterium Rhodococcus jostii RHA1 which was able to degrade the Kraft lignin via C(α)-C(β) bond cleavage ( Ahmad et al, 2011 ). Similarly, BaDyP, a dye decolourizing peroxidase from Bacillus amyloliquefaciens was found to decolourize dyes and degrade guaiacylglycerol-β-guaiacyl ether (GGE), a phenolic β-ether lignin model ( Yang et al, 2019 ).…”

Section: Methods Of Lignin Depolymerizationmentioning

confidence: 99%

Endophytes in Lignin Valorization: A Novel Approach

Mattoo

Nonzom

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Lignin, one of the essential components of lignocellulosic biomass, comprises an abundant renewable aromatic resource on the planet earth. Although 15%––40% of lignocellulose pertains to lignin, its annual valorization rate is less than 2% which raises the concern to harness and/or develop effective technologies for its valorization. The basic hindrance lies in the structural heterogeneity, complexity, and stability of lignin that collectively makes it difficult to depolymerize and yield common products. Recently, microbial delignification, an eco-friendly and cheaper technique, has attracted the attention due to the diverse metabolisms of microbes that can channelize multiple lignin-based products into specific target compounds. Also, endophytes, a fascinating group of microbes residing asymptomatically within the plant tissues, exhibit marvellous lignin deconstruction potential. Apart from novel sources for potent and stable ligninases, endophytes share immense ability of depolymerizing lignin into desired valuable products. Despite their efficacy, ligninolytic studies on endophytes are meagre with incomplete understanding of the pathways involved at the molecular level. In the recent years, improvement of thermochemical methods has received much attention, however, we lagged in exploring the novel microbial groups for their delignification efficiency and optimization of this ability. This review summarizes the currently available knowledge about endophytic delignification potential with special emphasis on underlying mechanism of biological funnelling for the production of valuable products. It also highlights the recent advancements in developing the most intriguing methods to depolymerize lignin. Comparative account of thermochemical and biological techniques is accentuated with special emphasis on biological/microbial degradation. Exploring potent biological agents for delignification and focussing on the basic challenges in enhancing lignin valorization and overcoming them could make this renewable resource a promising tool to accomplish Sustainable Development Goals (SDG’s) which are supposed to be achieved by 2030.

show abstract

Degradation of the phenolic β-ether lignin model dimer and dyes by dye-decolorizing peroxidase from Bacillus amyloliquefaciens

Cited by 31 publications

References 14 publications

Biotransformation of Lignin by an Artificial Heme Enzyme Designed in Myoglobin With a Covalently Linked Heme Group

Biotransformation of Lignin by an Artificial Heme Enzyme Designed in Myoglobin With a Covalently Linked Heme Group

Thymelaea hirsuta and Echinops spinosus: Xerophytic Plants with High Potential for First-Generation Biodiesel Production

Endophytes in Lignin Valorization: A Novel Approach

Contact Info

Product

Resources

About