Degradation of wool byHofmannophila pseudospretella (lepidoptera: Oecophoridae) larval midgut extracts under conditions simulating the midgut environment

Christeller, John T.

doi:10.1002/(sici)1520-6327(1996)33:2<99::aid-arch2>3.0.co;2-s

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…The curves for the two substrates were similar, suggesting that the activities are due to the same enzyme. These pH optima are within the H. pseudospretella larval midgut pH range of 8.5-9.2 (Christeller, 1996), as would be expected. The pH optima are also much higher than the value of pH 7.8 reported for the major H. pseudospretella leucine aminopeptidase (Christeller et al, 1994).…”

Section: Characterizationsupporting

confidence: 80%

Identification of isopeptidase activity in the midgut of insects: Purification, properties and nutritional ecology of a Hofmannophila pseudospretella (Lepidoptera: Oecophoridae) larval enzyme

Simpson

Christeller

2010

Insect Science

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A γ‐glutamyl transpeptidase (isopeptidase) has been purified 580‐fold to homogeneity from the midgut of keratinophagous larvae of Hofmannophila pseudospretella. The enzyme is a single polypeptide of molecular mass 80 kDa. The enzyme was identified by its hydrolytic activity against the synthetic substrate, γ‐glutamyl‐AMC, its molecular mass and inhibition profile compared to other γ‐glutamyl transpeptidases. The enzyme is low or absent from most other insect digestive systems apart from other keratinophagous lepidopteran larvae and predatory carabids. While isopeptide bonds are present in high levels of the proteins in the diet of keratinophages, their presence in the diet of predatory beetles has not been established.

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Section: Characterizationsupporting

confidence: 80%

Identification of isopeptidase activity in the midgut of insects: Purification, properties and nutritional ecology of a Hofmannophila pseudospretella (Lepidoptera: Oecophoridae) larval enzyme

Simpson

Christeller

2010

Insect Science

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“…Hofmannophila pseudospretella (Stainton) (Lepidoptera: Oecophoridae) (brown house moth) larvae are keratinophagous (Woodroffe 1951). They have a similarly low redox potential in the midgut as T. bisselliella but the pH is less alkaline (Christeller 1996). A microscopical examination of H. pseudospretella larval midgut was carried out, the material was cultured and the various isolatable bacteria were characterized.…”

Section: Introductionmentioning

confidence: 99%

“…The midgut of keratinophagous insects is an unusual environment for biotic existence. The pH varies between pH 7 and pH 12 (Waterhouse 1949), and the redox potential between −400 and −100 mV (Waterhouse 1952; Christeller 1996). Values this extreme are rare, although the redox potential in the rumen is similar (Smith and Hungate 1958).…”

Section: Introductionmentioning

confidence: 99%

Characterization of lactic acid bacteria in the larval midgut of the keratinophagous lepidopteran, Hofmannophila pseudospretella

Shannon

Attwood

Hopcroft

et al. 2008

Letters in Applied Microbiology

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Hofmannophila pseudospretella specimens studied by microscopy, the larval midgut contained an unidenti®ed micro-organism. Although not seen microscopically in midgut sections, bacteria were isolated from dissected midgut. Microscopy, carbohydrate utilization and ribosomal sequence data all separated the isolates into the same three classes. These were identi®ed as Lactococcus lactis, Carnobacterium piscicola and, tentatively, Bacillus subtilis, the ®rst two being facultative anaerobes and the latter, an aerobe. The bacteria were therefore not speci®cally adapted to the reducing conditions of the midgut.

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“…During sulfitolysis the disulphide bonds between polypeptide keratin chains cleave to S-sulfocysteine, and cysteine occurs. The reaction takes place under the alkaline conditions in the presence of inorganic sulphite produced by the fungus [32]. Sulfitolysis entails protein denaturation which facilitates the attack of keratinolytic proteases.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Sheep Wool Geotextiles Designed for Erosion Control

Broda¹

2019

Environmental Chemistry and Recent Pollution Control Approaches

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Wool geotextiles were formed from the meandrically arranged thick ropes and used as erosion control products. The geotextiles were installed in the experimental sites to protect the endangered slopes and the bank of ditches. Additionally, as a reinforcement of the soil, loose wool fibres were applied. The progress of wool biodegradation on the slope was investigated. Changes in the outer appearance, mechanical parameters, molecular structure and fibre morphology were analysed. Moreover, the nitrogen content in the soil and the effect of compounds released into soil on the grass growth were studied. The measurements revealed that the biodegradation starts at the cleavage of disulphide bonds, followed by disruption of the peptide bonds. Degradation is initiated in the outer cuticle and is followed by the decomposition of the inner cortical cells. During biodegradation, the nitrogen-rich compounds are released. The compounds act as an effective fertiliser which supports the growth of grass and significantly accelerates the greening of the slope.

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Degradation of wool byHofmannophila pseudospretella (lepidoptera: Oecophoridae) larval midgut extracts under conditions simulating the midgut environment

Cited by 10 publications

References 31 publications

Identification of isopeptidase activity in the midgut of insects: Purification, properties and nutritional ecology of a Hofmannophila pseudospretella (Lepidoptera: Oecophoridae) larval enzyme

Identification of isopeptidase activity in the midgut of insects: Purification, properties and nutritional ecology of a Hofmannophila pseudospretella (Lepidoptera: Oecophoridae) larval enzyme

Characterization of lactic acid bacteria in the larval midgut of the keratinophagous lepidopteran, Hofmannophila pseudospretella

Biodegradation of Sheep Wool Geotextiles Designed for Erosion Control

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