Degradation pathway of cephalosporin antibiotics by in vitro cultures of Lentinula edodes and Imleria badia

Dąbrowska, Monika; Muszyńska, Bożena; Starek, Małgorzata; Żmudzki, Paweł; Opoka, Włodzimierz

doi:10.1016/j.ibiod.2017.11.014

Cited by 32 publications

(22 citation statements)

References 35 publications

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“…Continuing the implementation of the subject our team (Dąbrowska et al, 2018) made research project about the ability of L. edodes ’s mycelium from in vitro cultures to degrade antibiotics. Authors showed that the examined mycelia take short time duration to remove the cefuroxime axetil from the medium.…”

Section: Resultsmentioning

confidence: 99%

Lentinula edodes Mycelium as Effective Agent for Piroxicam Mycoremediation

et al. 2019

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Pollution of the environment with inorganic and organic substances is one of the main problems in the world. For this reason, it is necessary to conduct researches for effective methods of biodegradation of xenobiotics, including drugs whose unmetabolized forms are introduced into the environment, especially into water. One possible solution to this problem may be the use of white rot fungi, such as Lentinula edodes . This is an edible species used in medicine because of its beneficial anti-cancer, hypocholesterolemic, hypotensive, hypoglycemic and antioxidant effects. Due to the fact that the mycelium of L. edodes produces enzymes with oxidizing properties that can degrade xenobiotics. The aim of the work was verification if in vitro cultures of L. edodes can be used for bioremediation of non-steroidal, anti-inflammatory drug: piroxicam. For this purpose, the in vitro culture of L. edodes was derived and the mycelial cultures of this species enriched with piroxicam were analyzed. The biodegradation pathway of piroxicam by L. edodes mycelium was carried out by the UPLC/MS/MS method. The degradation process of piroxicam was found to affect primarily the linker between the thiazine and the piperidine ring, leading to its oxidation and cleavage. Additionally, oxidation of the benzothiazine moiety was observed, leading to hydroxylation and oxidation of the phenyl ring as well as oxidation of the thiazine ring leading to partial or complete removal of the sulfonamide moiety. It seems that the degradation process led finally to 2-hydroxybenozquinone, which may be further oxidized to inorganic compounds. What’s more, concentration of piroxicam in in vitro cultures of L. edodes was not correlated with effectiveness of biodegradation of this compound – on each experimental series, the level of degradation was the same. The results confirm the possibility of using the investigated L. edodes mycelium for remediation of piroxicam.

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Section: Resultsmentioning

confidence: 99%

Lentinula edodes Mycelium as Effective Agent for Piroxicam Mycoremediation

et al. 2019

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“…A Waters eλ PDA detector was used to obtain chromatograms. The detailed UPLC–MS/MS analysis was carried out strictly in accordance with the methodology presented by Dąbrowska et al ( 2018 ).…”

Section: Methodsmentioning

confidence: 99%

“…The most important mechanism for the decomposition of xenobiotics by mushroom enzymes is related to the decomposition of lignin. Extracellular enzymes modifying lignin possess low substrate specificity; thus, they can decompose large amounts of highly resistant organic contaminants with a structure similar to that of lignin (Dąbrowska et al 2018 ; Mansur et al 2003 ). The major enzymes of the lignin degradation system are lignin peroxidase, laccase, manganese-dependent peroxidase, and enzymes producing H 2 O 2 , although not all ligninolytic fungi synthesize them to the same degree (Hofrichter 2002 ; Kirk and Farrell 1987 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the biodegradation of synthetic testosterone and 17α-ethynylestradiol using the edible mushroom Lentinula edodes

et al. 2018

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The mycelium of Lentinula edodes produces enzymes which may degrade xenobiotics including steroid hormones. The aim of the study was to determine whether the mycelium from in vitro cultures of L. edodes are able to degrade endocrine disruptors such as testosterone and 17α-ethynylestradiol. To prove the possibility of xenobiotics degradation, cultures of L. edodes were cultivated in an Oddoux liquid medium with the addition of synthetic 17α-ethynylestradiol and synthetic testosterone. The endocrine disruptors were extracted from the mycelium and determined qualitatively by RP-HPLC. The degradation products of testosterone and 17α-ethynylestradiol were identified using a UPLC/MS/MS analysis. Undegraded testosterone was determined at the amount of 2.97 mg/g dry weight but only in one of the L. edodes extracts from in vitro cultures supplemented with 50 mg of this compound. In turn, 17α-ethynylestradiol was not determined in any samples. Additionally in all extracts, mushroom sterols (ergosterol peroxide and ergosterol) were determined. Their total amounts were significantly lower in samples containing the abovementioned steroids than in extracts from mycelium L. edodes without the addition of steroid hormones. The results demonstrated that the mycelium of L. edodes can be used in the biodegradation process of a water environment contaminated with endocrine disruptors.Electronic supplementary materialThe online version of this article (10.1007/s13205-018-1458-x) contains supplementary material, which is available to authorized users.

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“…In 2001, about 1.5 million prescriptions for drugs containing terbinafine were issued in the US alone (Davis et al 2019). Mushroom cultures could also be utilized to remove xenobiotics from the environment (Kryczyk et al 2017;Dąbrowska et al 2018). Lentinula edodes was selected for the current study because of its unique nutritional and healing properties.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of the use of Lentinula edodes mycelium in terbinafine remediation

et al. 2020

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A detailed understanding of the fate of xenobiotics introduced into the environment and the mechanisms involved in their biotransformation, biodegradation, and biosorption is essential to improve the efficiency of remediation techniques. Mycoremediation is a form of bioremediation technique that has become increasingly popular in recent years as fungi are known to produce various effective extracellular enzymes that have the potential to neutralize a wide variety of xenobiotics released into the environment. Hence, mycoremediation appears to be a promising technique for the removal of a wide array of toxins and pharmaceutical residues from a damaged environment and wastewater. This study primarily aimed to investigate whether white-rot fungus (Lentinula edodes) can be utilized for the bioremediation of common antifungal agent terbinafine, which is mainly available in the market as powder or cream. The cultures of L. edodes were cultivated in the medium containing terbinafine powder or terbinafine 1% cream, each at a final concentration of 0.1 mg mL −1. The addition of terbinafine in powder form have a negative effect on biomass growth (p < 0.05). The total amount of terbinafine in the dry weight of mycelium after culture was estimated to be 7.63 ± 0.45 mg and 12.52 ± 2.46 mg for powder and cream samples, respectively. In addition, there were no traces of terbinafine in any of the samples of medium used for culturing L. edodes after the experimental duration period. The biodegradation products of terbinafine were identified for the first time using UPLC/MS/MS. The biodegradation of terbinafine resulted in the loss of 1-naphthylmethanol, which occurred via oxidative deamination, N-demethylation, or tert-butyl group hydroxylation. The results of the study demonstrate that L. edodes mycelium can be effectively used for the remediation of terbinafine.

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Degradation pathway of cephalosporin antibiotics by in vitro cultures of Lentinula edodes and Imleria badia

Cited by 32 publications

References 35 publications

Lentinula edodes Mycelium as Effective Agent for Piroxicam Mycoremediation

Lentinula edodes Mycelium as Effective Agent for Piroxicam Mycoremediation

Analysis of the biodegradation of synthetic testosterone and 17α-ethynylestradiol using the edible mushroom Lentinula edodes

Feasibility of the use of Lentinula edodes mycelium in terbinafine remediation

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