Degranulation Inhibitors from Medicinal Plants in Antigen-Stimulated Rat Basophilic Leukemia (RBL-2H3) Cells

Matsuda, Hisashi; Nakamura, Seikou; Yoshikawa, Masayuki

doi:10.1248/cpb.c15-00781

Cited by 36 publications

(19 citation statements)

References 60 publications

(71 reference statements)

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“…To the best of our knowledge, there are only a few reports about the effect of triterpenes on b-hexosaminidase release and its structure-activity relationship, whereas the structure-activity relationship of flavonoids, stilbenes, and curcuminoids have been summarized (Matsuda et al 2016). Triterpenes are also widely distributed in natural products, plants and mushrooms, and different types of triterpenes have been found in these materials.…”

Section: Discussionmentioning

confidence: 99%

Anti-allergic triterpenes isolated from olive milled waste

2017

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The degranulation of basophils stimulated by antigen is one of the allergic mechanisms of immediate hypersensitivity reactions. In a previous study, the β-hexosaminidase release of rat basophilic leukemia (RBL-2H3) cells stimulated by IgE cross-linking was specifically inhibited by ethanol extract of olive milled waste (OMW). OMW is a potential source of triterpenes, which show several biological activities. However, little is known about the effect of triterpenes in OMW on β-hexosaminidase release. Here we investigated anti-allergic triterpenes from the ethanol extract of OMW, and we discuss the structure-activity relationship. We subjected ethanol extract of OMW to activity-guided fractionation; this led to the isolation of five known triterpenes, i.e., maslinic acid (1), oleanolic acid (2), punicanolic acid (3), 2-O-acetylmaslinic acid (4), and epimaslinic acid (5) as well as one new triterpene, 11-oxo-maslinic acid (6). An anti-allergic assay revealed that 2-O-acetylmaslinic acid (4) and 11-oxo-maslinic acid (6) showed anti-allergic activity. Considering the structure-activity relationship of these triterpenes, it appears that the existence of a ketone group at C-11 or an acetyl group at C-2 would influence the anti-allergic activity.

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Section: Discussionmentioning

confidence: 99%

Anti-allergic triterpenes isolated from olive milled waste

2017

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“…β-Hexosaminidase is also stored in secretory granules of mast cells and basophils, and is also released concomitantly with histamine when mast cells are immunologically activated. Therefore, it is generally accepted that β-hexosaminidase is a degranulation marker of mast cells, and this assay has been used for the evaluation of antiallergic compounds as an alternative to passive cutaneous anaphylaxis reactions in laboratory animals [20]. …”

Section: The Biofunctional Effects Of Japanese and Anhui Chakamentioning

confidence: 99%

New biofunctional effects of the flower buds of Camellia sinensis and its bioactive acylated oleanane-type triterpene oligoglycosides

et al. 2016

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We review the biofunctional effects of the flower buds of Camellia sinensis and C. sinensis var. assamica, such as antihyperlipidemic, antihyperglycemic, antiobesity, and gastroprotective effects in vivo, and antiallergic, pancreatic lipase inhibitory, and amyloid β (Aβ) aggregation inhibitory activities in vitro. Although the biofunctional effects of tea leaves have been extensively studied, less attention has been given to those of the flowers and seeds of the tea plant. Our studies focused on the saponin constituents of the extracts of the flower buds of C. sinensis cultivated in Japan and China, and C. sinensis var. assamica cultivated in India, and we review their beneficial biofunctions for health promotion.

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“…However, studies on allergic reactions using LUVA cells are still limited. Although derived from basophilic leukemia cells in neonatal Wistar rats, RBL-2H3 cells share similar characteristics to mast cells, and therefore RBL-2H3 cells have been extensively used as a good model for mast cell IgE-mediated degranulation [ 10 – 13 ].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulation Effects of Schizonepeta tenuifolia Briq. on the IgE‐Induced Allergic Model of RBL‐2H3 Cells

Lin

Chen

Chiu

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Schizonepeta tenuifolia (ST) Briq. is a traditional herbal medicine commonly used to treat allergic skin diseases, where the inflammation process is closely related to symptom severity. This study aimed to explore the immunomodulatory effect of ST by using immunoglobulin E- (IgE-) stimulated RBL-2H3 cell cultures, a common cell line for studying mast cell degranulation and inflammatory cytokine release in vitro. After stimulating the RBL-2H3 cells with IgE, ST at concentrations of 10, 50, or 100 μg/mL was added to the cell cultures. Cell viability, inflammatory cytokines (IL-6, IL-13, IL-4, TNF-α, and IFN-γ), anti-inflammatory cytokine IL-10, and degranulation ability were examined 48 and 72 hours after administration of ST. The markers of inflammation and allergic reaction, IFN-γ, TNF-α, IL-4, and IL-6, were suppressed, especially after treatment with 100 μg/mL ST. However, the anti-inflammation marker IL-10 was also suppressed by ST. Trend analysis showed that a higher ST concentration was associated with lower IFN-γ and TNF-α levels. Moreover, degranulation of RBL-2H3 cells was assessed by measuring the release of β-hexosaminidase, which was suppressed by ST at 10 μg/mL. This study showed an immunomodulatory effect of ST at the cellular level and suggests the role of ST in treating allergic diseases.

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Degranulation Inhibitors from Medicinal Plants in Antigen-Stimulated Rat Basophilic Leukemia (RBL-2H3) Cells

Cited by 36 publications

References 60 publications

Anti-allergic triterpenes isolated from olive milled waste

Anti-allergic triterpenes isolated from olive milled waste

New biofunctional effects of the flower buds of Camellia sinensis and its bioactive acylated oleanane-type triterpene oligoglycosides

Immunomodulation Effects of Schizonepeta tenuifolia Briq. on the IgE‐Induced Allergic Model of RBL‐2H3 Cells

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