Dehalococcoides mccartyi NIT01, a novel isolate, dechlorinates high concentrations of chloroethenes by expressing at least six different reductive dehalogenases

Recent advances and trends of trichloroethylene biodegradation: A critical review

“…Bacterial isolates and mixed culture involved in anaerobic reductive dechlorination of TCE were summarized in Table 3 , and a phylogenetic tree was constructed with the identified bacterial isolates ( Figure 2A ). As for bacterial isolates, organohalide-respiring bacteria (OHRB) have been confirmed to be highly effective TCE degrading strains ( Fincker and Spormann, 2017 ), including species from Dehalococcoides ( Maymo-Gatell et al, 1997 ; Maymó-Gatell et al, 1999 ; Gushgari-Doyle and Alvarez-Cohen, 2020 ; Asai et al, 2022 ) and Candidatus Dehalogenimonas ( Chen et al, 2022 ), which can dechlorinate TCE to benign ethene. Besides, strains from Dehalobacter ( Holliger et al, 1998 ; Rupakula et al, 2015 ), Enterobacter ( Kang et al, 2012 ), Clostridium ( Lo et al, 2020 ; Lin et al, 2021 ), and Acidimicrobiaceae ( Ge et al, 2019 ) were also observed to effectively dechlorinate TCE ( Table 3 ).…”

“…Reductive dehalogenase (RDase) is a key enzyme in OHRB such as Dehalococcoides , Dehalobacter , and is up-regulated during anaerobic reductive dechlorination ( Zhang et al, 2020 ; Asai et al, 2022 ; Chen et al, 2022 ; Koner et al, 2022 ). RDase is a membrane-associated iron–sulfur protein containing an activated super nucleophilic form of the coenzyme vitamin B12, cob(I)alamin, which cleaves carbon-halogen bonds, and dechlorinates TCE via electroreduction ( Zhang et al, 2020 ; Yan et al, 2021 ).…”

“…Overall, pceA , tceA (TCE to DCE), tceA , dceA , vcrA , bvcA (cis-DCE to VC), and tceA , vcrA , bvcA (VC to ethene) are crucial RDases involved in TCE reductive dechlorination process ( Figure 2B ; Ise et al, 2011 ; Popat and Deshusses, 2011 ; Dugat-Bony et al, 2012 ; Zinder, 2016 ; Dolinova et al, 2017 ; Mao et al, 2019 ; Yan et al, 2021 ; Rossi et al, 2022b ). Furthermore, the recently discovered new strain Dehalococcoides mccartyi NIT01 contains 19 rdhA genes (including NIT01 - rdhA7 and rdhA13 ), which are basically the same as the encoded vcrA and pceA ( Asai et al, 2022 ). Moreover, Candidatus Dehalogenimonas etheniformans strain GP can combine formate and H 2 oxidation with the reduction of TCE, DCE isomers, and VC to benign ethene using acetate as the carbon source, and the cerA gene plays a crucial role ( Chen et al, 2022 ).…”

Dehalococcoides mccartyi strain NIT01 grows more stably in vessels made of pure titanium rather than the stainless alloy SUS304

“…The use of a C1 e − donor, such as formate, can minimize the anthropogenic disturbance of groundwater ecologies in combination with bioaugmentation using pure Dehalococcoides (Tomita et al, 2022). Of the various Dehalococcoides strains that have been successfully isolated, a few have shown rapid dechlorination of TCE (0.5–4.0 mM) to ETH within 10–45 days (Asai et al, 2021; Zhao & He, 2018). Furthermore, to the best of our knowledge, the maximum scale of the Dehalococcoides pure culture is 7 L for the strain NIT01 in batch mode (Asai et al, 2021) and 1 L for the strain CBDB1 in continuous mode (Reino et al, 2023).…”

“…Of the various Dehalococcoides strains that have been successfully isolated, a few have shown rapid dechlorination of TCE (0.5–4.0 mM) to ETH within 10–45 days (Asai et al, 2021; Zhao & He, 2018). Furthermore, to the best of our knowledge, the maximum scale of the Dehalococcoides pure culture is 7 L for the strain NIT01 in batch mode (Asai et al, 2021) and 1 L for the strain CBDB1 in continuous mode (Reino et al, 2023). The other Dehalococcoides strains, such as FL2, 195, and CG1, have been cultured at <1 L (He et al, 2007; Wang et al, 2014; Yan et al, 2021), and the large‐scale culture of pure Dehalococcoides has not been commercially available.…”