Dehydrins Patterns in Common Bean Exposed to Drought and Watered Conditions

Abstract

<jats:p>Drought is a major constraint for common bean (Phaseolus vulgaris L.) production in México. Dehydrins are constitutive or stress-induced proteins related with a protective role of membranes and macromolecules against denaturation, thus preventing loss of their function. In this work, seed production and patterns of dehydrins accumulation in leaves and pods were evaluated in common bean cv. ‘Otomí’ subjected to drought, as compared with well-irrigated plants. Drought applied at pod formation and seed filling (SF) reduced yield up to 57 %. An antibody against a consensus sequence present in most dehydrins allowed for dehydrin identification. Two dehydrins of 82 and 73 kDA turned up both in leaves and pods throughout all the evaluated conditions. Presumably, both dehydrins are constitutive in the ‘Otomí’ cultivar. These dehydrins showed higher expression than controls in leaves after 6 d of drought at seedling and SF stages, and in pods 6 d after drought had started at SF. Increased expression might provide better protection during early stages of seedling and seed development. Increments on 63, 36 and 22 kDa dehydrin expression in pods at late SF might coincide with plant developmental programs, which prepare seed for desiccation. Dehydrins of 158, 54, 46, and 41 kDa were detected in pods 10 d after floral opening as a transient response to drought stress in SF. These results indicate dehydrins are relevant during plant development, as well as during drought stress.</jats:p>

Evidence for genotypic differences among elite lines of common bean in the ability to remobilize photosynthate to increase yield under drought

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Plant biomarkers as early detection tools in stress management in food crops: a review

“…Data from several research studies has shown that plant biomarkers are differentially expressed at different stages of plant growth when exposed to the same stress condition. Castañeda-Saucedo et al ( 2014 ) reported differences in dehydrin accumulation at the seed filling and pod formation stages of common beans ( Phaseolus vulgaris L.) subjected to drought stress. In a similar experiment, Samarah and colleagues observed a more significant dehydrin accumulation in soybean seeds ( Glycine max L.) at the maturity stage compared to the developmental stage (Samarah et al 2006 ).…”

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“…Similar to the extent of yield-losses recorded in cereal crops, substantial yield reductions were reported in grain legume crops owing to their susceptibility to drought stress. For instance, drought stress was reported to cause up to a 50 % yield loss in several legume crops including soybean (Guttikonda et al 2014), common bean (Castañeda-Saucedo et al 2014), chickpea (Varshney et al 2014), and cowpea (Ahmed and Suliman 2010).…”