Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent

Savini, Isabella; Duflot, Sylvie; Avigliano, Luciana

doi:10.1042/0264-6021:3450665

Cited by 40 publications

(42 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DHA reduction was not associated with a decline in cellular GSH (Fig. 3C), thereby implying the involvement of GSH-independent DHA reductases [49,60]. This is also an important observation, as a decrease in the GSH pool would be expected to enhance arsenite toxicity [22,25].…”

Section: Figmentioning

confidence: 79%

“…AA content was measured by high-performance liquid chromatography with the UV detection wavelength set at 265 nm, as described in ref. 49, with minor modifications. The assay involved the use of a 15 cm 3 4.6 mm Discovery C-18, 5 lm column (Supelco, Bellefonte, PA) equipped with a Supelguard Discovery C-18 guard column (2 cm 3 4 mm, 5 lm).…”

Section: Measurement Of Aa Content By High-performance Liquid Chromatmentioning

confidence: 99%

See 1 more Smart Citation

U937 cell apoptosis induced by arsenite is prevented by low concentrations of mitochondrial ascorbic acid with hardly any effect mediated by the cytosolic fraction of the vitamin

et al. 2015

View full text Add to dashboard Cite

Arsenite directly triggers cytochrome c and Smac/DIABLO release in mitochondria isolated from U937 cells. These effects were not observed in mitochondria pre-exposed for 15 min to 10 µM L-ascorbic acid (AA). In other experiments, intact cells treated for 24-72 h with arsenite were found to die by apoptosis through a mechanism involving mitochondrial permeability transition. Pre-exposure (15 min) to low micromolar concentrations of AA and dehydroascorbic acid (DHA), resulting in identical cytosolic levels of the vitamin, had a diverse impact on cell survival, as cytoprotection was only observed after treatment with AA. Also the mitochondrial accumulation of the vitamin was restricted to AA exposure. An additional indication linking cytoprotection to the mitochondrial fraction of the vitamin was obtained in experiments measuring susceptibility to arsenite in parallel with loss of mitochondrial and cytosolic AA at different times after vitamin exposure. Finally, we took advantage of our recent findings that DHA potently inhibits AA transport to demonstrate that DHA abolishes all the protective effects of AA, under the same conditions in which the mitochondrial accumulation of the vitamin is prevented without affecting the overall cellular accumulation of the vitamin.

show abstract

Section: Figmentioning

confidence: 79%

Section: Measurement Of Aa Content By High-performance Liquid Chromatmentioning

confidence: 99%

U937 cell apoptosis induced by arsenite is prevented by low concentrations of mitochondrial ascorbic acid with hardly any effect mediated by the cytosolic fraction of the vitamin

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Indeed dehydroascorbic acid is preferentially accumulated in comparison to L-ascorbic acid in HaCaT, a human keratinocyte cell line. 34 Our experiments to determine whether dehydroascorbic acid was preferable to L-ascorbic acid for topical use failed to reveal any increase in skin levels of L-ascorbic acid with dehydroascorbic acid.…”

Section: Discussionmentioning

confidence: 99%

Topical L-Ascorbic Acid: Percutaneous Absorption Studies

et al. 2001

View full text Add to dashboard Cite

show abstract

“…An idealized cell could be considered as a sphere with a diameter of 15 lm, which results in a cell volume of 1.8 · 10 À12 l/cell. This value is approx-imate to the reported cell volume in mammalian cells [5,[17][18][19]. For the cell culture system, a typical cell culture experiment would have a cell density of 2.5 · 10 6 cells/ml.…”

Section: Mathematical Models Of Receptor Trafficking Networkmentioning

confidence: 66%

Cellular signaling is potentially regulated by cell density in receptor trafficking networks

Klipp

2007

FEBS Letters

View full text Add to dashboard Cite

Previous work has shown that receptor trafficking is a potential site for the control of signaling pathways. In most biological experiments, the ligand concentration and cell density vary within a wide range among different systems. However, there is less attention to systematically analyze how much cellular signal response is affected by cell densities. Here, we use a quantitative mathematical model to investigate signal responses in different receptor trafficking networks by simultaneous variations of ligand concentration and cell density. Computational analysis of the model revealed that receptor trafficking networks have potential sigmoid responses to ratio between ligand and surface receptor number per cell, which is a key factor to control the signaling responses in receptor trafficking networks. Furthermore, cell density also affects the robustness of dose-response curve upon the variation of binding affinity.

show abstract

Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent

Cited by 40 publications

References 24 publications

U937 cell apoptosis induced by arsenite is prevented by low concentrations of mitochondrial ascorbic acid with hardly any effect mediated by the cytosolic fraction of the vitamin

U937 cell apoptosis induced by arsenite is prevented by low concentrations of mitochondrial ascorbic acid with hardly any effect mediated by the cytosolic fraction of the vitamin

Topical L-Ascorbic Acid: Percutaneous Absorption Studies

Cellular signaling is potentially regulated by cell density in receptor trafficking networks

Contact Info

Product

Resources

About