Dehydroepiandrosterone and neurotrophins favor axonal growth in a sensory neuron–keratinocyte coculture model

Ulmann, Lauriane; Rodeau, Jean‐Luc; Danoux, Louis; Contet-Audonneau, J.-L.; Pauly, G.; Schlichter, Rémy

doi:10.1016/j.neuroscience.2009.01.018

Cited by 34 publications

(21 citation statements)

References 61 publications

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“…The strong neurotrophic effect of urothelial cells was similar to the trophic effects of keratinocytes on sensory neurons 8,24 . Our preliminary attempts to identify the factor responsible showed that tyrosine kinase but not TrkA signalling was involved.…”

Section: Discussionmentioning

confidence: 76%

Co-Cultures Provide a New Tool to Probe Communication Between Adult Sensory Neurons and Urothelium

2013

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Purpose Recent evidence suggests the urothelium functions as a sensory transducer of chemical, mechanical or thermal stimuli that signals to nerve terminals and other cells in the bladder wall. The cellular and molecular basis of neuro-urothelial communication is not easily studied in the intact bladder, which led us to establish a method of co-culturing dorsal root ganglion (DRG) sensory neurons and bladder urothelial cells. Materials and Methods Sensory neurons and urothelial cells obtained from DRG and bladders dissected from adult female Sprague-Dawley rats were isolated by enzyme treatment and mechanical dissociation. These were plated together, or separately, on collagen-coated substrate and cultured in keratinocyte medium for 48–72 h. Retrograde tracer labeling was performed to identify bladder afferents used for functional testing. Results Neurite growth and complexity in neurons co-cultured with urothelial cells was increased relative to neuronal monocultures. The growth-promoting effect of urothelial cells was reduced by the tyrosine kinase inhibitor, K252a, but upstream inhibition of NGF signaling with TrkA-Fc had no effect. Fura-2 calcium imaging of urothelial cells showed responses to ATP (100μM) and activation of TRPV4 (4alpha-PDD, 10μM), but not TRPV1 (capsaicin, 1μM), TRPV3 (farnesyl pyrophosphate, 1μM), or TRPA1 (mustard oil, 100μM). In contrast, co-cultured neurons were activated by all agonists except farnesyl pyrophosphate. Conclusions Co-culturing provides a new methodology for investigating neuro-urothelial interactions in animal models of urological conditions. Our results suggest that neuronal properties are not only maintained in the presence of urothelium but that neurite growth is potentiated by an NGF-independent mechanism.

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Section: Discussionmentioning

confidence: 76%

Co-Cultures Provide a New Tool to Probe Communication Between Adult Sensory Neurons and Urothelium

2013

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“…Neurite length analysis was carried out according to the method described in previous studies [5, 26, 27]. We used the basic criteria for assessing neurite length: only the maximal distance along a neurite was defined as the neurite length; if there was more than one neurite, the distance from the soma to the end of the longest neurite was adopted; if there were branched neurites, the distance from the soma to the end of the longest branch at each branch-point was adopted.…”

Section: Methodsmentioning

confidence: 99%

CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation

et al. 2016

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Electrical stimulation (ES)-triggered up-regulation of brain-derived neurotrophic factor (BDNF) and neurite outgrowth in cultured rat postnatal dorsal root ganglion neurons (DRGNs) is calcium (Ca2+)-dependent. The effects of increased Ca2+ on BDNF up-regulation and neurite outgrowth remain unclear. We showed here that ES increased phosphorylation of the cAMP-response element binding protein (CREB). Blockade of Ca2+ suppressed CREB phosphorylation and neurite outgrowth. Down-regulation of phosphorylated (p)-CREB reduced BDNF transcription and neurite outgrowth triggered by ES. Furthermore, blockade of calmodulin-dependent protein kinase II (CaMKII) using the inhibitors KN93 or KN62 reduced p-CREB, and specific knockdown of the CaMKIIα or CaMKIIβ subunit was sufficient to suppress p-CREB. Recombinant BDNF or hyperforin reversed the effects of Ca2+ blockade and CaMKII knockdown. Taken together, these data establish a potential signaling pathway of Ca2+-CaMKII-CREB in neuronal activation. To our knowledge, this is the first report of the mechanisms of Ca2+-dependent BDNF transcription and neurite outgrowth triggered by ES. These findings might help further investigation of complex molecular signaling networks in ES-triggered nerve regeneration in vivo.

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“…DHEA is released concurrently with cortisol during physical stress (Charney 2004;Izawa et al 2008), protecting the body against the negative effects of prolonged exposure to glucocorticoids (Morgan et al 2004). Moreover, DHEA can act centrally to decrease glucocorticoid-induced neuronal death in the hippocampus and to promote neurogenesis in the dentate gyrus (DG) of the hippocampus and in sensory dorsal root ganglion neurons (Maninger et al 2009;Pinnock et al 2009;Ulmann et al 2009). Furthermore, the ratio between DHEA and cortisol has been found to be a reliable index of neuroprotection (Maninger et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Behavioral training and predisposed coping strategies interact to influence resilience in male Long-Evans rats: Implications for depression

Bardi

Rhone

Franssen

et al. 2012

Stress

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Effective coping strategies and adaptive behavioral training build resilience against stress-induced pathology. Both predisposed and acquired coping strategies were investigated in rats to determine their impact on stress responsiveness and emotional resilience. Male Long-Evans rats were assigned to one of the three coping groups: passive, active, or variable copers. Rats were then randomly assigned to either an effort-based reward (EBR) contingent training group or a non-contingent training group. Following EBR training, rats were tested in appetitive and stressful challenge tasks. Physiological responses included changes in fecal corticosterone and dehydroepiandrosterone (DHEA) metabolites as well as neuropeptide Y (NPY)-immunoreactivity in the hippocampus and amygdala. Regardless of a rat's predisposed coping strategy, EBR rats persisted longer than non-contingent rats in the appetitive problem-solving task. Furthermore, training and coping styles interacted to yield the seemingly most adaptive DHEA/corticosterone ratios in the EBR-trained variable copers. Regardless of training group, variable copers exhibited increased NPY-immunoreactivity in the CA1 region.

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Dehydroepiandrosterone and neurotrophins favor axonal growth in a sensory neuron–keratinocyte coculture model

Cited by 34 publications

References 61 publications

Co-Cultures Provide a New Tool to Probe Communication Between Adult Sensory Neurons and Urothelium

Co-Cultures Provide a New Tool to Probe Communication Between Adult Sensory Neurons and Urothelium

CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation

Behavioral training and predisposed coping strategies interact to influence resilience in male Long-Evans rats: Implications for depression

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