Dehydroepiandrosterone (DHEA) such as 17β‐estradiol prevents MPTP‐induced dopamine depletion in mice

D’Astous, Myreille; Morissette, Marc; Tanguay, Bastien; Callier, Sophie; Paolo, Thérèse Di

doi:10.1002/syn.10145

Cited by 94 publications

(64 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although BFP contains phytoestrogens such as ginseng 37) that may act similarly to estrogen, which has been shown to prevent neuronal degeneration caused by increased oxidative stress 38) and apoptosis 39) ; other component herbs of BFP may act through different mechanisms, the details of which remain to be elucidated. Consistent with our research, TH and DAT gene expression 40,41) and protein patterns 41,42) of dopaminergic neurons have also been described by the other researchers. The intensity of TH-and DAT-immunoreactive dopaminergic cell bodies and terminals was also decreased in the substantia nigra and striatum of mice after MPTP treatment.…”

Section: Discussionsupporting

confidence: 93%

Neuroprotective Effects of Bak Foong Pill in 1-Methyl-4-phenyl-1,2,3,6-tetrahyrdropyridine (MPTP)-Induced Parkinson's Disease Model Mice

Liu

Xie

Rowlands

et al. 2004

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Parkinsons's disease (PD) is a chronic neurodegenerative disorder characterized by the loss of dopaminergic neurons of substantia nigra pars compacta in the ventral midbrain.1)The loss of dopaminergic neurons leads to the reduction of dopamine release into the striatum, and is responsible for the clinical features of PD including bradykinesia, resting tremor, rigidity, and difficulty in initiating movements. 2)Pathological patterns of PD are usually examined by inducing Parkinson's like symptoms in rodents by injection of the dopaminergic neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and is widely used for study of PD. 3)MPTP is converted to MPP ϩ , which structurally resembles dopamine, by brain monoamine oxidase B (MAO-B) and can be actively transported into the dopaminergic nerve terminals of the striatum by dopamine transporter (DAT).4) It is important to note that DAT is found only in dopaminergic neurons and may be the best and only unique marker for dopaminergic neurons.5) It has been shown that administration of MPP ϩ alone decreases dopamine transporter protein expression by Western blot. 6) In addition, MPTP intoxication has been reported to be associated with reduced immunoreactivity of tyrosine hydroxylase (TH), the rate-limiting enzyme of dopamine synthesis, 7) in the dopaminergic neurons of substantia nigra via the dopamine uptake system. This is considered to lead to energy failure and subsequently the inhibition of protein expression.8) The reduction of TH-positive neurons is also considered to be a good marker for dopaminergic cell loss.Current therapy for PD is essentially symptomatic, with the use of levodopa, the direct precursor of dopamine, remaining the primary treatment choice since its first use over 30 years ago.9-11) However, long-term therapy with levodopa is associated with significant side effects.12) Neuroprotective therapy to rescue dopamine neurons, which could alter and prevent the progression of PD, 13) has thus been proposed as an alternative to symptomatic treatment. It has been shown that neuroprotection can be observed in the PD models with iron chelators, radical scavenger antioxidants, MAO-B inhibitors, nitric oxide synthase inhibitors, calcium channel antagonists, glutamate antagonist trophic factors.12) Even nicotine and low exposure to cigarette smoke may have a neuroprotective effect on the dopaminergic nigrostriatal system. 14)In addition, epidemiological data suggest that steroid hormone 17b-estradiol plays an important role in protecting the brain from neurodegenerative processes, including Parkinson's disease. Estrogen, but not androgen, has been reported to prevent MPTP-induced dopamine depletion, 15) decrease of DAT mRNA and DAT specific binding.16) Phytoestrogens 6,17) and Chinese medicines, 18) as well as acupuncturing without the use of any drug 19) have also been demonstrated to have neuroprotective effects in PD. As a well-known gynaecological tonic in China, Bak Foong Pill (BFP, China registration #Z980035, also known as Bai Feng Wan) has ...

show abstract

Section: Discussionsupporting

confidence: 93%

Neuroprotective Effects of Bak Foong Pill in 1-Methyl-4-phenyl-1,2,3,6-tetrahyrdropyridine (MPTP)-Induced Parkinson's Disease Model Mice

Liu

Xie

Rowlands

et al. 2004

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…Along these lines, a number of studies have demonstrated that estrogen is neuroprotective in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced nigrostriatal lesions, an animal model of idiopathic PD [130][131][132][133][134][135][136][137][138][139][140]. Estrogen prevented MPTP-induced depletion of striatal dopamine, dopamine transporter binding and expression, decreased the MPTP-induced tyrosine hydroxylase-immunoreactive neuron loss and attenuated the glial activation induced by MPTP [130][131][132][133][134][135][136][137][138][139][140]. The estrogenic steroid principally used in these studies was 17β-estradiol (E2), and its effect on dopamine loss was shown by several studies to be stereospecific, as an isomer with weak estrogenic activity, 17α-estradiol, was ineffective at preventing MPTP-induced dopamine loss [131][132][133].…”

Section: Parkinson's Diseasementioning

confidence: 99%

Neurotrophic and neuroprotective actions of estrogen: Basic mechanisms and clinical implications

et al. 2007

View full text Add to dashboard Cite

Estrogen is an important hormone signal that regulates multiple tissues and functions in the body. This review focuses on the neurotrophic and neuroprotective actions of estrogen in the brain, with particular emphasis on estrogen actions in the hippocampus, cerebral cortex and striatum. Sex differences in the risk, onset and severity of neurodegenerative disease such as Alzheimer's disease, Parkinson's disease and stroke are well known, and the potential role of estrogen as a neuroprotective factor is discussed in this context. The review assimilates a complex literature that spans research in humans, non-human primates and rodent animal models and attempts to contrast and compare the findings across species where possible. Current controversies regarding the WHI (Women's Health Initiative) study, its ramifications, concerns and the new studies needed to address these concerns are also addressed. Signaling mechanisms underlying estrogen-induced neuroprotection and synaptic plasticity are reviewed, including the important concepts of genomic versus nongenomic mechanisms, types of estrogen receptor involved and their subcellular targeting, and implicated downstream signaling pathways and mediators. Finally, a multicellular mode of estrogen action in the regulation of neuronal survival and neurotrophism is discussed, as are potential future directions for the field.

show abstract

“…25) In addition, as shown in Table 3, the levels of DA, DOPAC, 3-MT, HVA and 5-HT in the control mouse striatum measured by the HPLC-ECD method revealed significantly different values in different experiments, which are possibly due to different electrochemical behaviors and effects of analytes under different applied potentials and mobile phase compositions. [26][27][28]30,31) For example, the levels of DA reported by different workers were approximately in the range of 11 to 110 ng.…”

Section: Application Of the Methods To Real Biological Samplesmentioning

confidence: 98%

“…[25][26][27][28] However, the HPLC-ECD method has low sensitivity and serious limitations in the simultaneous determination of monoamines such as 5-HT, DA and DOPAC. 29) Under the standard separation protocols, the HPLC-ECD method requires two different mobile phases along with two separated HPLC-ECD systems for measuring the levels of 5-HT, DA and their metabolites.…”

Section: Application Of the Methods To Real Biological Samplesmentioning

confidence: 99%

Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Park

Myung

Kim

et al. 2013

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

In order to measure the levels of serotonin (5-hydroxyltryptamine, 5-HT), dopamine (DA), 3,4-hydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT) and homovanillic acid (HVA) simultaneously, an effective derivatization method followed by high-performance liquid chromatography (HPLC) coupled to electrochemical ionization mass spectrometry was used. The derivatization reaction of biological samples with ethyl chloroformate occurred rapidly at room temperature in aqueous conditions, and the resulting derivatives were isocratically separated with good selectivity using a C18 reversed-phase column within 30 min. The study results showed that the new derivatization procedure offers an excellent means of simultaneous determination of 5-HT, DA and their metabolites in mouse brain homogenates, which are important in a number of physiological and behavioral functions.Key words neurotransmitter; HPLC-MS; ethyl chloroformate; derivatization Dopamine (DA) and serotonin (5-hydroxyltryptamine, 5-HT), which are widely distributed as a monoamine neurotransmitter in the central nervous system (CNS), 1) play important physiological and behavioral roles such as the control and regulation of affective behavior, sleep, reward system, motor and cognitive functions.2) Changes of DA and 5-HT basal levels have been linked to some CNS-related disorders such as depression, panic disorder, schizophrenia and Parkinson's disease (PD).2,3) PD is pathologically characterized by the death of dopaminergic neurons in the substantia nigra pars compacta 4) and caused by neurotoxins such as 1-methyl-4-phenyl-1,2,3,6-tetrahypyridine (MPTP), which produces a clinical syndrome similar to PD. Because the administration of MPTP is known to affect the dopaminergic neurons in the nigrostriatal system with subsequent depletion of DA and its metabolites, 5,6) the measurement of 5-HT, DA and their metabolites is necessary to discover and test new drugs for treating these disorders. 7)Various analytical methods have been widely investigated for determining the level of monoamine neurotransmitters and their metabolites, including high-performance liquid chromatography (HPLC) coupled with electrochemical detection (ECD), dual ECD, fluorescence detection (FLD), FLD/ECD system, chemiluminescence detection and mass spectrometer (MS) and gas chromatography (GC) coupled with MS in biological samples such as plasma, rat brain, and mouse brain and in vitro experiments.8-10) HPLC-ECD, which is the most common analytical technique used to determine monoamines and their metabolites, has the advantages of low cost and fast speed 11) but maintaining its routine use is problematic due to sudden noise problems.12) The HPLC-FLD method is used to measure fluorescent derivatives of monoamine neurotransmitters by a derivatization procedure, 13) which was performed by benzylamine and 1,2-diphenylethylenediamine in a two-step reaction in weakly alkaline media and in the presence of potassium hexacyanoferrate (III).14) However, since this protocol requires the vigorous reaction ...

show abstract

Dehydroepiandrosterone (DHEA) such as 17β‐estradiol prevents MPTP‐induced dopamine depletion in mice

Cited by 94 publications

References 35 publications

Neuroprotective Effects of Bak Foong Pill in 1-Methyl-4-phenyl-1,2,3,6-tetrahyrdropyridine (MPTP)-Induced Parkinson's Disease Model Mice

Neuroprotective Effects of Bak Foong Pill in 1-Methyl-4-phenyl-1,2,3,6-tetrahyrdropyridine (MPTP)-Induced Parkinson's Disease Model Mice

Neurotrophic and neuroprotective actions of estrogen: Basic mechanisms and clinical implications

Simultaneous Measurement of Serotonin, Dopamine and Their Metabolites in Mouse Brain Extracts by High-Performance Liquid Chromatography with Mass Spectrometry Following Derivatization with Ethyl Chloroformate

Contact Info

Product

Resources

About