Deinococcus terrigena sp. nov., a novel member of the family Deinococcaceae

Abstract

A bacterial strain, S13-1-2-1, was isolated from a soil sample collected in Gyeongsangnam-do province, South Korea. Cells were observed to be Gram-stain negative, short rod-shaped and colonies to be pale pink in colour. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Deinococcus in the family Deinococcaceae, with high levels of sequence similarity with Deinococcus ficus CC-FR2-10 (97.9%) and Deinococcus enclensis NIO-1023 (95.4%). Growth of strain S13-1-2-1 was observed at 10-42 °C, pH 6-8, and in the presence of 0-1.0% NaCl. The isolate was found to exhibit resistance to gamma radiation (D 10.1 KGy) and UV-light (D 612 J/m). The major peptidoglycan amino acids were identified as D-glutamic acid, glycine, alanine and L-ornithine. The predominant respiratory quinone of the strain was identified as menaquinone-8, the major fatty acids were found to be C ω7c (31.4%), C (18.4%), and C ω8c (17.4%) and the major polar lipids were observed to be an unidentified phosphoglycolipid and an unidentified glycolipid. The genomic DNA G + C content of the strain was determined to be 69.2 mol%. DNA-DNA hybridization with D. ficus showed a relatedness value of 31.5 ± 4.2%. The DNA-DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain S13-1-2-1 represents a novel species in the genus Deinococcus, for which the name Deinococcus terrigena sp. nov. is proposed. The type strain is S13-1-2-1 (= KCTC 33939 = JCM 32248).

Genomics-based identification of a cold adapted clade in Deinococcus

“…Thus, for phylogenomic clustering, complete genomes of Meiothermus cerbereus DSM 11376 (GCA_000620065.1) and Meiothermus sp. QL-1 (GCA_003351145.1) were chosen as the outgroup species as they are close relatives of Deinococcus [ 46 ]. With the two Meiothermus and 85 Deinococcus genomes, a maximum likelihood phylogenomic tree was constructed using PhyloPhlAn3 with default options [ 47 ].…”

Deinococcus betulae sp. nov. and Deinococcus arboris sp. nov., novel bacteria isolated from bark of birch tree (Betula platyphylla)

“…The major polar lipids are glycolipid and phosphoglycolipid, whereas phosphatidylglycerol or diphosphatidylglycerol are absent. The predominant fatty acids are unbranched 15, 16, and 17 carbon acids and branched 16 and 17 carbon acids in mesophilic and thermophilic members, respectively [3–5]. Members of the genus have been isolated from a variety of environments, such as air, soil, desert, water, tree, hot spring, sludge, food, marine fish and animal gut [6, 7].…”