Deinococcus wulumuqiensis sp. nov., and Deinococcus xibeiensis sp. nov., isolated from radiation-polluted soil

Abstract

The taxonomic positions of two gamma- and UV-ray-resistant strains isolated from radiation-polluted soil in north-west China were determined in a polyphasic study. The organisms, designated R12T and R13T, were Gram-stain-positive, non-spore-forming cocci, which contained MK-8 as the major respiratory quinone and C16 : 1 ω7c and C16 : 0 as major fatty acids. The cell walls of strains R12T and R13T contained ornithine. Phylogenetic analysis based on 16S rRNA gene sequences and DNA–DNA hybridizations showed that strains R12T and R13T are members of novel species belonging to the genus Deinococcus, with Deinococcus radiodurans DSM 20539T as the closest relative. The isolates R12T and R13T shared 97 and 97.1 % 16S rRNA gene similarity, respectively, and 29.5 and 33.3 % DNA–DNA relatedness, respectively, with D. radiodurans DSM 20539T. The DNA G+C contents of isolates R12T and R13T were 66.7 and 63.8 %, respectively. On the basis of phenotypic tests and other results, two species, Deinococcus wulumuqiensis sp. nov. (type strain R12T =CGMCC 1.8884T =NBRC 105665T) and Deinococcus xibeiensis sp. nov. (type strain R13T =CGMCC 1.8885T =NBRC 105666T), are proposed.

Highly Selective Oxidation of 5-Hydroxymethylfurfural to 5-Hydroxymethyl-2-Furancarboxylic Acid by a Robust Whole-Cell Biocatalyst

“…Similar to the prototype strain of the Deinococcus genus, D. radiodurans R1, D. wulumuqiensis R12 is also well known for its excellent ability to resist extremely high doses of gamma and UV radiation [45]. In order to explore its potential applications in biocatalysis, growing and resting cells of this strain were applied as biocatalysts in the conversion of HMF to form HMFCA.…”

“…Nevertheless, employing whole-cells or isolated enzymes derived from extremophiles for biocatalysis in a general manner is just beginning to be implemented experimentally. Recently, a Deinococcus sp, designated as Deinococcus wulumuqiensis R12, was isolated from radiation-polluted soil [45,46]. Previous studies showed that it is phylogenetically more closely related to a prototype strain of the Deinococcus genus, namely Deinococcus radiodurans R1 [47].…”

Genome Sequence of a Gamma- and UV-Ray-Resistant Strain, Deinococcus wulumuqiensis R12

“…It was isolated from the radiation-contaminated soils in the Xinjiang Uigur Autonomous Region of northwest China. Strain R12 is a Gram-positive, reddish orange, non-spore-forming coccus, with gamma radiation resistance to >10 kGy and UV resistance to >700 J m -2 (7). This strain shows a higher tolerance for gamma radiation and UV light than does D. radiodurans R1.…”

Draft Genome Sequence of Deinococcus xibeiensis R13, a New Carotenoid-Producing Strain

“…Strain R13 is an aerobic, Gram-positive, red-pigmented coccus with gamma-radiation resistance to >10 kGy and UV resistance to >700 J · m -2 (7). R13 produces deinoxanthin, and its color is deeper than that of D. radiodurans .…”