Delayed fluorescence spectra of intact leaves photoexcited by sunlight measured with a multichannel Fourier-transform chemiluminescence spectrometer

Akita, Saeka; Yano, Ayako; Ishii, Hiroshi; Satoh, Chikahiro; Akai, Nobuyuki; Nakata, Munetaka

doi:10.1016/j.cplett.2013.04.062

Cited by 6 publications

(4 citation statements)

References 20 publications

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“…Rougui was used as a control for pigment and ultra-structural analyses. For Chl analysis, we first performed a quick measurement of the relative amount of Chl content using a SPAD-502PLUS Chl meter (Spectrum Technologies, Konica Minolta, Japan) according to a previous report (Akita et al, 2013 ). Then, total Chl and carotenoids were extracted and quantified using a TU-1810PC spectrophotometer (Purkinje General Instrument Co. Ltd., Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

De novo Sequencing of the Leaf Transcriptome Reveals Complex Light-Responsive Regulatory Networks in Camellia sinensis cv. Baijiguan

Chen

Sun

et al. 2016

Front. Plant Sci.

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Tea plants (Camellia sinensis L.) possess high genetic diversity that is important for breeding. One cultivar, Baijiguan, exhibits a yellow leaf phenotype, reduced chlorophyll (Chl) content, and aberrant chloroplast structures under high light intensity. In contrast, under low light intensity, the flush shoot from Baijiguan becomes green, the Chl content increases significantly, and the chloroplasts exhibit normal structures. To understand the underlying molecular mechanisms for these observations, we performed de novo transcriptome sequencing and digital gene expression (DGE) profiling using Illumina sequencing technology. De novo transcriptome assembly identified 88,788 unigenes, including 1652 transcription factors from 25 families. In total, 1993 and 2576 differentially expressed genes (DEGs) were identified in Baijiguan plants treated with 3 and 6 days of shade, respectively. Gene Ontology (GO) and pathway enrichment analyses indicated that the DEGs are predominantly involved in the ROS scavenging system, chloroplast development, photosynthetic pigment synthesis, secondary metabolism, and circadian systems. The light-responsive gene POR (protochlorophyllide oxidoreductase) and transcription factor HY5 were identified. Quantitative real-time PCR (qRT-PCR) analysis of 20 selected DEGs confirmed the RNA-sequencing (RNA-Seq) results. Overall, these findings suggest that high light intensity inhibits the expression of photosystem II 10-kDa protein (PsbR) in Baijiguan, thus affecting PSII stability, chloroplast development and chlorophyll biosynthesis.

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Section: Methodsmentioning

confidence: 99%

De novo Sequencing of the Leaf Transcriptome Reveals Complex Light-Responsive Regulatory Networks in Camellia sinensis cv. Baijiguan

Chen

Sun

et al. 2016

Front. Plant Sci.

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“…To assess the efficiency of the photosynthetic apparatus of plants and its resistance to various external influences, methods were developed for considering the intensity of the delayed fluorescence (DF) [ 69 , 70 , 71 , 72 , 73 , 74 ].…”

Section: Herbicide-absorption and Translocation In The Soil-plants Systemsmentioning

confidence: 99%

Pesticides: Behavior in Agricultural Soil and Plants

Bondareva

Fedorova

2021

Molecules

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This review considers potential approaches to solve an important problem concerning the impact of applied pesticides of various classes on living organisms, mainly agricultural crops used as food. We used the method of multi-residual determination of several pesticides in agricultural food products with its practical application for estimating pesticides in real products and in model experiments. The distribution of the pesticide between the components of the soil-plant system was studied with a pesticide of the sulfonylureas class, i.e., rimsulfuron. Autoradiography showed that rimsulfuron inhibits the development of plants considered as weeds. Cereals are less susceptible to the effects of pesticides such as acetamiprid, flumetsulam and florasulam, while the development of legume shoots was inhibited with subsequent plant death.

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“…The usefulness of DF from PSII for various applications has been summarized by Guo and Tan [ 12 ], which include assessment or detection of photosynthesis rate, plant circadian, plant senescence, nutrients, salt stress, chilling stress, heat stress, drought stress, acid rain, herbicide, metal pollution, and aquatic ecosystems. An attempt to analyze DF in the frequency domain can be found in Guo and Tan [ 13 ]; however, DF from PSII has been mainly analyzed in the time domain [ 12 , 14 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

On the Potential Usefulness of Fourier Spectra of Delayed Fluorescence from Plants

Guo

Tan

2014

Sensors

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Delayed fluorescence (DF) from photosystem II (PSII) of plants can be potentially used as a biosensor for the detection of plant physiological status and environmental changes. It has been analyzed mainly in the time domain. Frequency-domain analysis through Fourier transform allows viewing a signal from another angle, but the usefulness of DF spectra has not been well studied. In this work, experiments were conducted to show the differences and similarities in DF spectra of different plants with short pulse excitation. The DF spectra show low-pass characteristics with first-order attenuation of high frequencies. The results also show that the low-frequency components differ while the high-frequency components are similar. These may imply the potential usefulness of Fourier spectra of DF to analyze photoelectron transport in plants and classify samples.

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Delayed fluorescence spectra of intact leaves photoexcited by sunlight measured with a multichannel Fourier-transform chemiluminescence spectrometer

Cited by 6 publications

References 20 publications

De novo Sequencing of the Leaf Transcriptome Reveals Complex Light-Responsive Regulatory Networks in Camellia sinensis cv. Baijiguan

De novo Sequencing of the Leaf Transcriptome Reveals Complex Light-Responsive Regulatory Networks in Camellia sinensis cv. Baijiguan

Pesticides: Behavior in Agricultural Soil and Plants

On the Potential Usefulness of Fourier Spectra of Delayed Fluorescence from Plants

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