Delayed functional maturation of neonatal porcine islets in recipients under strict glycemic control

Abstract

These results suggest that tight control of glycemia reduces the functional maturation of NPI grafts.

Fibrin supports subcutaneous neonatal porcine islet transplantation without the need for pre‐vascularization

“…33,50,54 Alternately, Pepper et al 43 The rational for successful engraftment of NPI plus fibrin in the subcutaneous space without the need for pre-vascularization or proangiogenic growth factors is due in part to NPI resistance to (a) hypoxia 57 ; (b) human pro-inflammatory cytokines 58 ; and (c) hyperglycemia. 18 For example, De Mesmaeker et al 44…”

“…There is a strong rationale to pursue the use of porcine donors for clinical islet xenotransplantation, including (a) the unlimited availability of porcine islets, increasing access to islet transplants and eliminating waiting time 17 ; (b) the reproducibility and quality of preparing porcine islets, predictably high and not compromised by co-morbidity, brain death, and ischemia related to human islets [17][18][19] ; (c) porcine insulin has been used to treat human diabetes for more than 60 years; 17 (d) porcine islets respond to glucose in the same physiological range as do human islets; (e) new techniques allow genetic manipulation and cloning of pigs, if it proves necessary or advantageous to do so 19,20 ; and (f) porcine islets are a potential therapy for highly allo-sensitized patients. 21 In addition, we have previously described a simple, inexpensive, and reproducible method to isolate large numbers of neonatal porcine islets (NPIs).…”

Xenotransplantation literature update May–August, 2007

“…Kin and Kurbutt studied the impact of glycemic control on maturation of neonatal porcine islets after transplantation under the kidney capsule of diabetic severe combined immunodeficient mice [55]. They observed that neonatal porcine islets functioned more efficiently when transplanted simultaneously with allogeneic islets.…”

Co‐transplantation of human adipose‐derived mesenchymal stem cells with neonatal porcine islets within a prevascularized subcutaneous space augments the xenograft function

“…A limited supply of cadaveric donor islets and human islet quality can range greatly between donors, which are major hurdles to the islet transplantation becoming the standard of care. There is strong rationale to pursue the use of porcine donors for clinical islet xenotransplantation, including (a) the reproducibility and quality of preparing porcine islets, probably high and not compromised by co‐morbidity, brain death, and ischemia related to human islets; (b) the limitless availability of porcine islets, increasing access to islet transplants, and eliminating waiting time; (c) new techniques allow genetic manipulation and cloning of pigs, if it proves necessary or advantageous to do so; and (d) porcine islets are a potential therapy for highly allosensitized patients . In addition, we have previously described a simple, inexpensive, and reproducible method to isolate large numbers of neonatal porcine islets .…”