Delayed hypersensitivity and lesions following isoimmunization with modified rat male accessory glands: kinetics of induction

Experimental rodent models of prostatitis: limitations and potential

“…Histological changes in the target organs (coagulating glands and prostatecseminal vesicle) were evident in almost all animals examined at 35, 42 and 49 days after the first injection of modified MAG and were characterized by inflammatory reactions and small perivascular mononuclear infiltrates. 87 Thus, this model is consistent with a self-limiting mild to moderate prostate inflammation induced by both humoral and cell-mediated mechanisms. 88 It has been speculated that inflammatory damage induced in this model might be the result of generation of autoreactive cytotoxic cells, increased levels of radical oxidative species from prostatic infiltrating macrophages, and upregulated mast cell degranulation.…”

“…85 High percentage (B70-80%) of male syngeneic recipients exhibited inflammation in the prostate; however, no humoral immune responses were detected in these recipients after cell transfer of MNC from donors with MAG-induced prostatitis. 86 Galmarini et al 87 used 3 months old male Wistar rats immunized with chemically modified MAG (MAG was coupled to diazonium derivates of arsenilic and sulphanilic acid which act as adjuvant) once, twice or three times on days 0, 30 and 45. Inoculation of MAG was necessary to confer higher titers of antibody.…”

Non-obese Diabetic (NOD) Mice are Genetically Susceptible to Experimental Autoimmune Prostatitis (EAP)

“…The possibility of inducing prostatitis in laboratory animals by experimental manipulation of their immune system further supports this view. Thymectomy at birth [4], oestradiol administration which affects local antigen expression [5] or direct immunization with prostatic antigens in complete Freund's adjuvant (CFA) [6,7] result in typical histological infiltrates, similar to those seen in human patients [8].…”

“…Over the last decade, our laboratory has developed a rat model of experimental autoimmune prostatitis (EAP) induced by autoimmunization of young adult Wistar males, with a male accessory gland (MAG) extract in CFA [6]. Infiltrating mononuclear cells appear at approximately day 7 after priming.…”

Prostatein or Steroid Binding Protein (PSBP) Induces Experimental Autoimmune Prostatitis (EAP) in NOD Mice

“…It is based upon the injection into young adult Wistar males with a male accessory gland global extract together with complete FreundЈs adjuvant (CFA) (4,5).…”