Delayed Hypersensitivity in Mice to Dextran

Crowle, Alfred J.; Hu, Cai

doi:10.1159/000229861

Cited by 16 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, the role of polysaccharides in delayed-type hypersensitivity has been shown by several workers [3,6,11,15]. The present study revealed that purified antigenic polysaccharides of A. fumigatus and C. albicans, containing a negligible quantity of nitrogen, are able to act as both inducers and elicitors of delayed-type skin reactions and Table 7.…”

Section: Discussionsupporting

confidence: 61%

“…In contrast, however, a few papers have recently showed that some preparations of Leuconostoc dextran [3,6] and pneumococcal polysaccharide [11], all of which contain negligible amounts of nitrogen, are capable of producing delayed hypersensitivity.…”

mentioning

confidence: 98%

“…Polysaccharides consisting of a simple carbohydrate have generally been considered to be inactive in inducing delayed hypersensitivity. In contrast, however, a few papers have recently showed that some preparations of Leuconostoc dextran [3,6] and pneumococcal polysaccharide [11], all of which contain negligible amounts of nitrogen, are capable of producing delayed hypersensitivity.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Skin Reaction and Macrophage Migration Inhibition Tests for Polysaccharides from Aspergillus fumigatus and Candida albicans

Suzuki

Hayashi

1975

Japanese Journal of Microbiology

View full text Add to dashboard Cite

Guinea pigs sensitized with purified galactomannan from Aspergillus fumigatus and mannan from Candida albicans, each containing negligible quantities of nitrogen, were examined for their immunological responses against the corresponding polysaccharides with respect to the delayed-type skin reaction and the macrophage migration inhibition phenomenon.In both cases, the delayed-type skin reaction test and the macrophage migration inhibition test showed positive results. The reactivity was stronger in animals sensitized with polysaccharides in Freund's complete adjuvant than those sensitized with the same polysaccharides in Freund's incomplete adjuvant.Polysaccharides chemically modified by partial acid degradation or by periodate oxidation were found to be completely incapable of eliciting such immune responses. These results are also discussed in relation to the antigenic determinant of the polysaccharides in such immune responses and the precipitin reaction previously observed by us and other investigators.

show abstract

Section: Discussionsupporting

confidence: 61%

mentioning

confidence: 98%

mentioning

confidence: 99%

See 1 more Smart Citation

Skin Reaction and Macrophage Migration Inhibition Tests for Polysaccharides from Aspergillus fumigatus and Candida albicans

Suzuki

Hayashi

1975

Japanese Journal of Microbiology

View full text Add to dashboard Cite

show abstract

“…B. fragilis CP is capable of functioning as a T-independent antigen in the classical manner of other polysaccharides as demonstrated by the ability of this antigen to stimulate antibody in athymic rats. There is much less evidence to support the concept that these antigens can stimulate a T celldependent response, although reports of induction of such responses do exist (23)(24)(25). The antigen used for immunization in the current studies is a polysaccharide and contains no detectable protein.…”

Section: With F Varium Plus Enterococcus Resulted In Abscesses Scessmentioning

confidence: 91%

Evidence for T Cell-dependent Immunity to Bacteroides fragilis in an Intraabdominal Abscess Model

Onderdonk¹,

Markham²,

Zaleznik³

et al. 1982

J. Clin. Invest.

View full text Add to dashboard Cite

A B S T R A C T It has been shown that active immunization of rats with the capsular polysaccharide of Bacteroides fragilis protects these animals against abscess development following intraperitoneal challenge with this species. Passive transfer of hyperimmune globulin from immunized animals to nonimmune recipients provided protection against B. fragilis bacteremia in challenged animals, but did not confer protection against abscess development. On the other hand, adoptive transfer of spleen cells from immunized animals to nonimmunized recipients resulted in protection against abscesses following challenge with B. fragilis. These data suggested that a T cell-dependent immune response was involved in protection against abscess development after immunization with B. fragilis capsular antigen.To determine the possible role of cell-mediated immunity prompted by the capsular antigen, inbred congenitally athymic OLA/Rnu rats and their phenotypically normal littermates were actively immunized. Despite the development of high titers of anti-B. fragilis capsular antibody, 100% of actively immunized athymic rats developed abscesses, as did 100% of unimmunized athymic control rats. However, no phenotypically normal littermate control rats that were actively immunized developed abscesses, while 100% of phenotypically normal unimmunized rats developed abscesses. Additional studies showed that adoptive transfer of T cell-enriched spleen cell preparations Dr.

show abstract

“…These studies have included skin tests, dye extravasation (1)(2)(3)(4)(5) and caliper measurements of paw swelling (6). Studies of hypersensitivity reactions in mice are particularly suitable because of the large number of inbred strains available and the economy of maintenance.…”

mentioning

confidence: 99%

Analysis of Dextran- and Methylated Albumin-Induced Hypersensitivity by Mouse Paw Swelling

Uyeki

Klassen

Llácer

1969

Experimental Biology and Medicine

View full text Add to dashboard Cite

Numerous studies have been conducted on immediate and delayed hypersensitivity in mice. These studies have included skin tests, dye extravasation (1)(2)(3)(4)(5) and caliper measurements of paw swelling (6). Studies of hypersensitivity reactions in mice are particularly suitable because of the large number of inbred strains available and the economy of maintenance. Van Arman et al. (7) and Winter et al. (8) have utilized a plethysmographic measurement for the study of irritative agents on swelling of foot paws of rats. This procedure also provides a novel, quantitative, and delicate method for studying dermal allergic reactions in mice, as was shown by the present studies which were designed to answer two questions: Can plethysmographic measurements be performed with precision and reliability on volumes as small as mouse paws; will immunological swelling of mouse paws be of sufficient magnitude to enable one to quantitatively assess the degree of reactivity? In large measure these questions have been answered in our present study.The present communication deals with (i) the application of the plethysmographic method to the study of small volumes, (ii) experiments dealing with the precision and reliability of the method, and (iii) the application of the method to the study of mouse paw sweIling induced by dextran and methylated human serum albumin (MHSA) , agents which produce edema as a result of previous immunization. This technique permits one to distinguish between swelling induced by a toxic or irritative component and swelling immunologically induced by an agent. Recently, Axelrad (9) described an assay for delayed hypersensitivity by immersing rat paw in a mercury-filled beaker placed on a top-loading balance. Materials and Methods.Male LAFl (C57L X A/He) mice purchased from Jackson Memorial Laboratories, Bar Harbor, Maine, 6-16 weeks of age, were used for these experiments. Native dextran, 5-40 milion in molecular weight, and crystalline human serum albumin were purchased from Nutritional Biochemicals, Cleveland, Ohio. Methylated human serum albumin (MHSA) was prepared according to the procedure of Crowle and Hu ( 5 ) . Microliter syringes for subplantar injections were purchased from Hamilton Co., Whittier, California.Plethysmograph measurement of small volumes. The plethysmographic instrumentation was a modification of the method of Van Arman et al. (7). The apparatus consists of a plastic cup filled with mercury in which mouse paws are immersed; small pressure changes, caused by displacement of the mercury level by the immersed object, are pro-portionaIly converted by a low-pressure transducer to electrical signals which are recorded on a chart recorder. The instrument consisted of the following: (i) Gilson model M5 polygraph, Gilson Medical Electronics, Middleton, Wisconsin. (ii) Statham model P23BB transducer, 0.5 cm Hg, Statham Laboratories, Inc., Hato Rey, Puerto Rico. (iii) Plastic cup of 12 mm i.d. The size of the cup was of a convenient diameter to allow for paw immersion without touching the si...

show abstract

Delayed Hypersensitivity in Mice to Dextran

Cited by 16 publications

References 21 publications

Skin Reaction and Macrophage Migration Inhibition Tests for Polysaccharides from Aspergillus fumigatus and Candida albicans

Skin Reaction and Macrophage Migration Inhibition Tests for Polysaccharides from Aspergillus fumigatus and Candida albicans

Evidence for T Cell-dependent Immunity to Bacteroides fragilis in an Intraabdominal Abscess Model

Analysis of Dextran- and Methylated Albumin-Induced Hypersensitivity by Mouse Paw Swelling

Contact Info

Product

Resources

About