Delayed inhibition of ERK and p38 attenuates neuropathic pain without affecting motor function recovery after peripheral nerve injury

Huang, Saisai; Chen, Ying-Ting; Jia, Yue; Yang, Tuo; Su, Wenfeng; Zhu, Zhenyu; Xue, Peng; Feng, FeiFan; Zhao, Yayu; Chen, Gang

doi:10.1016/j.neuropharm.2021.108835

Cited by 8 publications

(2 citation statements)

References 46 publications

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“…Besides, in our sciatic crush injury mice the basic motor function tested by Footprint could easily revered about 1 month while complicated motor action potentials examined by Rotarod test were slightly recovered even after 3 months (Fig. 1 A, B), which is consistent with our previous results that the compound muscle action potentials (CMAPs) not completely restored even after 56 days [ 60 ]. It might due to the different strategies to construct sciatic nerve crush models, such as the distinct tools, time and force for extrusion lead to different injury degrees and recovery period.…”

Section: Discussionsupporting

confidence: 91%

Maresin 1 promotes nerve regeneration and alleviates neuropathic pain after nerve injury

Wei

Zhao

et al. 2022

J Neuroinflammation

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Background Peripheral nerve injury (PNI) is a public health concern that results in sensory and motor disorders as well as neuropathic pain and secondary lesions. Currently, effective treatments for PNI are still limited. For example, while nerve growth factor (NGF) is widely used in the treatment of PNI to promote nerve regeneration, it also induces pain. Maresin 1 (MaR1) is an anti-inflammatory and proresolving mediator that has the potential to regenerate tissue. We determined whether MaR1 is able to promote nerve regeneration as well as alleviating neuropathic pain, and to be considered as a putative therapeutic agent for treating PNI. Methods PNI models were constructed with 8-week-old adult male ICR mice and treated with NGF, MaR1 or saline by local application, intrathecal injection or intraplantar injection. Behavioral analysis and muscle atrophy test were assessed after treatment. Immunofluorescence assay was performed to examine the expression of ATF-3, GFAP, IBA1, and NF200. The expression transcript levels of inflammatory factors IL1β, IL-6, and TNF-α were detected by quantitative real-time RT-PCR. AKT, ERK, mTOR, PI3K, phosphorylated AKT, phosphorylated ERK, phosphorylated mTOR, and phosphorylated PI3K levels were examined by western blot analysis. Whole-cell patch-clamp recordings were executed to detect transient receptor potential vanilloid 1 (TRPV1) currents. Results MaR1 demonstrated a more robust ability to promote sensory and motor function recovery in mice after sciatic nerve crush injury than NGF. Immunohistochemistry analyses showed that the administration of MaR1 to mice with nerve crush injury reduced the number of damaged DRG neurons, promoted injured nerve regeneration and inhibited gastrocnemius muscle atrophy. Western blot analysis of ND7/23 cells cultured with MaR1 or DRG neurons collected from MaR1 treated mice revealed that MaR1 regulated neurite outgrowth through the PI3K–AKT–mTOR signaling pathway. Moreover, MaR1 dose-dependently attenuated the mechanical allodynia and thermal hyperalgesia induced by nerve injury. Consistent with the analgesic effect, MaR1 inhibited capsaicin-elicited TRPV1 currents, repressed the nerve injury-induced activation of spinal microglia and astrocytes and reduced the production of proinflammatory cytokines in the spinal cord dorsal horn in PNI mice. Conclusions Application of MaR1 to PNI mice significantly promoted nerve regeneration and alleviated neuropathic pain, suggesting that MaR1 is a promising therapeutic agent for PNI.

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Section: Discussionsupporting

confidence: 91%

Maresin 1 promotes nerve regeneration and alleviates neuropathic pain after nerve injury

Wei

Zhao

et al. 2022

J Neuroinflammation

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“…JNK and p38 cause neuronal apoptosis, while ERK1/2 is in charge of neural survival [146][147][148]. Nevertheless, it has been reported that ERKs contribute in the emergence of neuropathic pain [149,150]. Many investigations using STZ-diabetic rats have shown that the neurons in the spinal ganglia are overexpressed in ERK, p38, and JNK [151].…”

Section: Mapk Signaling Pathwaymentioning

confidence: 99%

Recent Advances in Biomolecular Patho-Mechanistic Pathways behind the Development and Progression of Diabetic Neuropathy

Ratan,

Rajput,

Pareek

et al. 2024

Biomedicines

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Diabetic neuropathy (DN) is a neurodegenerative disorder that is primarily characterized by distal sensory loss, reduced mobility, and foot ulcers that may potentially lead to amputation. The multifaceted etiology of DN is linked to a range of inflammatory, vascular, metabolic, and other neurodegenerative factors. Chronic inflammation, endothelial dysfunction, and oxidative stress are the three basic biological changes that contribute to the development of DN. Although our understanding of the intricacies of DN has advanced significantly over the past decade, the distinctive mechanisms underlying the condition are still poorly understood, which may be the reason behind the lack of an effective treatment and cure for DN. The present study delivers a comprehensive understanding and highlights the potential role of the several pathways and molecular mechanisms underlying the etiopathogenesis of DN. Moreover, Schwann cells and satellite glial cells, as integral factors in the pathogenesis of DN, have been enlightened. This work will motivate allied research disciplines to gain a better understanding and analysis of the current state of the biomolecular mechanisms behind the pathogenesis of DN, which will be essential to effectively address every facet of DN, from prevention to treatment.

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Study on analgesic effect of Shentong Zhuyu Decoction in neuropathic pain rats by network pharmacology and RNA-Seq

Wang,

Lin,

Xie

et al. 2024

Journal of Ethnopharmacology

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Delayed inhibition of ERK and p38 attenuates neuropathic pain without affecting motor function recovery after peripheral nerve injury

Cited by 8 publications

References 46 publications

Maresin 1 promotes nerve regeneration and alleviates neuropathic pain after nerve injury

Maresin 1 promotes nerve regeneration and alleviates neuropathic pain after nerve injury

Recent Advances in Biomolecular Patho-Mechanistic Pathways behind the Development and Progression of Diabetic Neuropathy

Study on analgesic effect of Shentong Zhuyu Decoction in neuropathic pain rats by network pharmacology and RNA-Seq

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