“…However, live birth via artificial insemination has only been achieved in the koala and tammar wallaby and was conducted with fresh, not frozen and thawed, sperm (Rodger et al., 2009). The tammar wallaby, a highly regimented and reliable breeder with a seasonal anestrus, had a notably low success rate (Paris et al., 2005b; Witt, Hinds, et al., 2018). To overcome underlying factors potentially precluding conventional artificial insemination, such as poor sperm motility (Rodger, 2019), ICSI may provide a generic tool.…”