Delayed shrinkage triggered by the Na<sup>+</sup>–K<sup>+</sup> pump in terbutaline‐stimulated rat alveolar type II cells

Hosoi, Keita; Min, Kyung‐Jin; Iwagaki, Akitaka; Murao, Hiroshi; Hanafusa, Toshiaki; Shimamoto, Chikao; Katsu, Ken‐ichi; Kato, Masumi; Fujiwara, Shoko; Nakahari, Takashi

doi:10.1113/expphysiol.2003.026617

Cited by 11 publications

(28 citation statements)

References 28 publications

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“…These procedures have been previously reported to provoke PMP hyperpolarization in diverse types of cells [Sharp and Debnam, 1994;Spindler et al, 1998;Schweigel et al, 1999;Hosoi et al, 2004;Petkov et al, 2005;Liang et al, 2008] In particular, in cultured BCE cells substitution of extracellular sodium by choline, in the absence of bicarbonate (as in the present study), was shown to provoke sustained hyperpolarization [Jentsch et al, 1984]. Also, in bullfrog corneal endothelium, valinomycin has been employed to produce lasting hyperpolarizations [Graves and Sachs, 1982].…”

Section: Hyperpolarization Proceduresmentioning

confidence: 60%

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture

Nin

Hernández

Chifflet

2009

Cell Motil. Cytoskeleton

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In previous works we showed that the depolarization of the plasma membrane potential (PMP) determines a reorganization of the cytoskeleton of diverse epithelia in culture, consisting mainly of a reallocation of peripheral actin toward the cell center, ultimately provoking intercellular disruption. In view of this evidence, we explored in this study the possible effects of membrane potential hyperpolarization on the cytoskeletal organization and adherens junction (AJ) morphology and the stability of confluent bovine corneal endothelial cells in culture. For this purpose, hyperpolarization was achieved by substitution of extracellular sodium by nondiffusible cations or via the incorporation of valinomycin to the control solution. Actin compactness at the cell periphery was assessed by quantitative analysis of fluorescence microscopy images. The stability of the AJ was challenged by calcium deprivation or temperature decrease. Our results showed that plasma membrane hyperpolarization provokes a compaction of AJ-associated actin filaments toward the plasma membrane and an increase in the stability of the AJs. We also observed that the hyperpolarizing procedures determined similar modifications in the actin cytoskeleton of endothelial cells in whole bovine corneas. Together with our previous work, the results of this study contribute to the idea that modifications in the PMP of nonexcitable cells participate in cellular adaptive responses involving reorganization of cytoskeletal components.

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Section: Hyperpolarization Proceduresmentioning

confidence: 60%

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture

Nin

Hernández

Chifflet

2009

Cell Motil. Cytoskeleton

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“…All reagents were dissolved in DMSO and diluted to their final concentrations just before the experiments. The concentration of DMSO never exceeded 0.1%; at this concentration, DMSO does not affect exocytotic events in antral mucous cells (6,7,11,21).…”

Section: Methodsmentioning

confidence: 88%

“…This cover slip was set in a perfusion chamber mounted on the stage of a differential interference contrast microscope (BX50Wi; Olympus, Tokyo, Japan) connected to a video-enhanced contrast system (AR-GUS-10; Hamamatsu Photonics, Hamamatsu, Japan; see Refs. 6,7,10,11,14,20,21,28). Images were recorded continuously using a video recorder.…”

Section: Methodsmentioning

confidence: 99%

cGMP modulation of ACh-stimulated exocytosis in guinea pig antral mucous cells

Saad¹,

Shimamoto²,

Nakahari³

et al. 2006

American Journal of Physiology-Gastrointestinal and Liver Physi

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In guinea pig antral mucous cells, ACh stimulates the Ca2+-regulated exocytosis, which has a characteristics feature: an initial transient phase followed by a sustained phase. The effects of cGMP on ACh-stimulated exocytosis were studied in guinea pig antral mucous cells using video microscopy. cGMP enhanced the frequency of ACh-stimulated exocytotic events, whereas cGMP alone did not induce any exocytotic events under the ACh-unstimulated condition. cGMP did not stimulate either Ca2+ mobilization or cAMP accumulation. The Ca2+ dose-response studies demonstrated that cGMP shifted the dose-response curve upward with no shift to the lower concentration. This indicates that cGMP increased maximal responsiveness of the Ca2+-regulated exocytosis, but not the Ca2+ sensitivity. Moreover, under a condition of ATP depletion by dinitrophenol (DNP) or anoxia (N2 bubbling), ACh evoked only a sustained phase in exocytotic events with no initial transient phase. However, ACh evoked an initial transient phase followed by a sustained phase with addition of cGMP before ATP depletion, whereas only a sustained phase was evoked in a case of cGMP addition after ATP depletion. Thus cGMP-induced enhancement in ACh-stimulated exocytotic events requires ATP, suggesting that cGMP modulates ATP-dependent priming of Ca2+-regulated exocytosis in antral mucous cells. In conclusion, cGMP increases the number of primed granules via acceleration of the ATP-dependent priming, which enhances the Ca2+-regulated exocytosis stimulated by ACh.

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“…Various agonists induce isosmotic cell shrinkage by activating K ϩ and Cl Ϫ channels in epithelial cells, such as salivary acinar cells (5,18), lung cells (11,14,16,17,23), sweat gland cells (30), and bronchiolar ciliary cells (26). The cell shrinkage modulates some cellular functions, such as ion transport (23,32), apoptosis (13), ciliary beat frequency (26), and exocytosis (6).…”

mentioning

confidence: 99%

“…The DMSO concentration did not exceed 0.1%. At this concentration, DMSO has no effect on cell volume and exocytotic events (6,7,11,15,19,20,(25)(26)(27)(28). Cell preparation.…”

mentioning

confidence: 99%

[Cl⁻]_imodulation of Ca²⁺-regulated exocytosis in ACh-stimulated antral mucous cells of guinea pig

Shimamoto¹,

Umegaki²,

Katsu³

et al. 2007

American Journal of Physiology-Gastrointestinal and Liver Physi

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The effects of intracellular Cl− concentration ([Cl−]i) on acetylcholine (ACh)-stimulated exocytosis were studied in guinea pig antral mucous cells by video microscopy. ACh activated Ca2+-regulated exocytosis (an initial phase followed by a sustained phase). Bumetanide (20 μM) or a Cl− -free (NO3−) solution enhanced it; in contrast, 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, a Cl− channel blocker) decreased it and eliminated the enhancement induced by bumetanide or NO3− solution. ACh and Ca2+ dose-response studies demonstrated that NO3− solution does not shift their dose-response curves, and ATP depletion studies by dinitrophenol or anoxia demonstrated that exposure of NO3− solution prior to ATP depletion induced an enhanced initial phase followed by a sustained phase, whereas exposure of NO3− solution after ATP depletion induced only a sustained phase. Intracellular Ca2+ concentration ([Ca2+]i) measurements showed that bumetanide and NO3− solution enhanced the ACh-stimulated [Ca2+]i increase. Measurements of [Cl−]i revealed that ACh decreases [Cl−]i and that bumetanide and NO3− solution decreased [Cl−]i and enhanced the ACh-evoked [Cl−]i decrease; in contrast, NPPB increased [Cl−]i and inhibited the [Cl−]i decrease induced by ACh, bumetanide, or NO3− solution. These suggest that [Cl−]i modulates [Ca2+]i increase and ATP-dependent priming. In conclusion, a decrease in [Cl−]i accelerates ATP-dependent priming and [Ca2+]i increase, which enhance Ca2+-regulated exocytosis in ACh-stimulated antral mucous cells.

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Delayed shrinkage triggered by the Na⁺–K⁺ pump in terbutaline‐stimulated rat alveolar type II cells

Cited by 11 publications

References 28 publications

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture

cGMP modulation of ACh-stimulated exocytosis in guinea pig antral mucous cells

[Cl⁻]_imodulation of Ca²⁺-regulated exocytosis in ACh-stimulated antral mucous cells of guinea pig

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Delayed shrinkage triggered by the Na+–K+ pump in terbutaline‐stimulated rat alveolar type II cells

Cited by 11 publications

References 28 publications

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture

cGMP modulation of ACh-stimulated exocytosis in guinea pig antral mucous cells

[Cl−]imodulation of Ca2+-regulated exocytosis in ACh-stimulated antral mucous cells of guinea pig

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Delayed shrinkage triggered by the Na⁺–K⁺ pump in terbutaline‐stimulated rat alveolar type II cells

[Cl⁻]_imodulation of Ca²⁺-regulated exocytosis in ACh-stimulated antral mucous cells of guinea pig