Delayed Type Hypersensitivity to Allogeneic Cells in Mice

Smith, Frank; Jf, Miller

doi:10.1159/000232205

Cited by 16 publications

(8 citation statements)

References 9 publications

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“…DTH to allogeneic cells in the mouse is a T cell-me diated, transferable, MHC-restricted response to cell surface antigens coded by the H-2 complex [21,22]. Sensitised spleen cells effectively induced DTH when transferred to naive, irradiated mice, but CsA treat ment of donor mice led to the production of suppres sor spleen cells which could block DTH when trans ferred together with sensitised cells (table I).…”

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confidence: 99%

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Characterisation of Cyclosporine-Induced Suppressor Cells in Murine Delayed-Type Hypersensitivity Responses

Mirisklavos¹,

Mottram²,

Dumble³

et al. 1986

Int Arch Allergy Immunol

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The effect of Cyclosporine A (CsA) on T cell-mediated delayed-type hypersensitivity (DTH) reactions to murine alloantigens was analysed by transferring spleen cells from sensitised and suppressed mice into irradiated naive recipients. Selective removal of Thy1+, Ly1+ or Ly2+ cells prior to intravenous transfer of cells from alloantigen-sensitised mice showed that Ly1+ Thy1+ cells transferred sensitivity. Concomitant treatment of mice with alloantigen and CsA suppressed the DTH response to alloantigens, and spleen cells transferred from these suppressed mice abrogated the response of sensitised cells transferred at the same time. The suppressor cells were strain-restricted and antigen-specific with the surface phenotype Thy + , Ly1–– Ly2+.

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confidence: 99%

“…Smith and Miller [22] also demon strated that DTH sensitivity to allogeneic cell surface antigens could be transferred to naive mice by intra venous injection of particular subsets of T lympho cytes obtained from mice sensitised to allogeneic cells.…”

Section: -------------------------------------------mentioning

confidence: 99%

Characterisation of Cyclosporine-Induced Suppressor Cells in Murine Delayed-Type Hypersensitivity Responses

Mirisklavos¹,

Mottram²,

Dumble³

et al. 1986

Int Arch Allergy Immunol

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“…Cells of the Lyl+ T cell subset mediate murine DTH to alloantigens and the reactions are transfer able, major histocompatibility (MHC)-restricted re sponses to cell surface antigens coded by the H-2 complex [24,25], The Lyl+ T cells can induce skin graft rejection when transferred into T cell deficient mice [22] but their mode of action is controversial; they may actively induce a specific immune response and/or act indirectly to recruit cytotoxic Ly2+ T cells and monocytes [22,26,27], Recent studies have shown that CsA blocks the de velopment of antigen-reactive (sensitised) T cells, by blocking antigen-induced secretion of IL-2 and other growth factors required for T cell activation [7][8][9][10]. In some studies, CsA could not block the action of T cells already activated by antigen [5,6,13,14].…”

Section: Discussionmentioning

confidence: 99%

Effects of Cyclosporin A, Antilymphocyte Serum and Donor-Specific Transfusions on Murine Delayed-Type Hypersensitivity and Skin Graft Survival

Mottram¹,

Mirisklavos²,

Dumble³

et al. 1986

Int Arch Allergy Immunol

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The effect of immunosuppressive reagents cyclosporin A (CsA) and rabbit anti-mouse antilymphocyte serum (ALS) on the response to alloantigens was studied in inbred mouse strains. Alloantigen was given either as a cell suspension which induced a delayed-type hypersensitivity reaction (DTH), or as a full-thickness skin graft. Dose-response studies showed that DTH reactions in CBA mice sensitised to BALB/c cells were reduced to background levels when recipient mice were treated with 100 mg/kg CsA on days 0, 4 and 6 after primary alloantigenic challenge. The response to a second challenge was significantly decreased by CsA treatment during primary or secondary exposure to alloantigen and CsA was as effective as ALS in abrogating both primary and secondary DTH reactions. Survival of full-thickness grafts of BALB/c skin on CBA mice was increased from 9 to 23 days by ALS treatment on days ––1 and +2, with grafts given on day 0. Long-term treatment with CsA, from day ––14 to +12, also prolonged graft survival from 9 to 18 days but donor-specific transfusions, with or without concomitant ALS or CsA treatment, decreased graft survival and often sensitised the recipients. This occurred with transfusions administered from ––63 to ––7 days and on the day of grafting. Thus, in H-2 mismatched mice, both CsA and ALS treatments produced a state of tolerance when administered during short-term exposure to alloantigen. With a stronger antigenic stimulus, in the form of a skin graft, ALS was only partially effective in overcoming the sensitising effects of donor-specific transfusions given prior to grafting and CsA could not prevent sensitisation of the recipient, but the continued presence of the drug delayed the onset of rejection in unsensitised recipients.

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“…Concerning the Lyt phenotypes of the cells mediating DTH reactions, it has been reported that T cells responsible for DTH against a soluble antigen or red blood cells are of the Lyt 1 subpopulation (26,12), whereas the cells for DTH against allogeneic cells and viral antigens, are of the Lyt 2 subpopulation (22,14). Moreover, in regard to the Lyt subclasses for MIF production, Adelman et al reported that in mice primed with a soluble antigen, the Lyt 1 subpopulation produced most of the MIF after in vitro antigen stimulation, whereas the Lyt 2 subpopulation produced little if any (1).…”

Section: Discussionmentioning

confidence: 99%

Production by Cultured Spleen Cells of Inflammatory Substances and Other Lymphokines that Mediate Delayed‐Type Hypersensitivity in Mice

Tamura

Tsuru

Chiba

et al. 1982

Microbiology and Immunology

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In vitro cultivation of normal mouse spleen cells with human serum albumin generated effector cells that mediate the delayed-type hypersensitivity (DTH) reaction. The cultured cells, when incubated in a serum-free medium for a further 24 hr, released substances (FPRF) which caused a footpad inflammatory reaction at a maximum of 6 hr after injection into normal syngeneic or allogeneic strains of mice, as well as macrophage migration inhibition factor (MIF) and macrophage activating factor (MAF). The DTH-effector cells in the culture were fractionated in the low density layers by discontinuous bovine serum albumin density gradient centrifugation. The effector cells in the low density layers were further enriched in the Lyt 1 subpopulation of T cells when fractionated on a fluorescence activated cell sorter. Cells capable of producing the inflammatory substances (FPRF), MIF and MAF were also enriched in the same fraction containing DTH-effector cells. These results suggest that low density, Lyt I-positive T cells mediating the DTH reaction produce FPRF as well as MIF and MAF.

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Delayed Type Hypersensitivity to Allogeneic Cells in Mice

Cited by 16 publications

References 9 publications

Characterisation of Cyclosporine-Induced Suppressor Cells in Murine Delayed-Type Hypersensitivity Responses

Characterisation of Cyclosporine-Induced Suppressor Cells in Murine Delayed-Type Hypersensitivity Responses

Effects of Cyclosporin A, Antilymphocyte Serum and Donor-Specific Transfusions on Murine Delayed-Type Hypersensitivity and Skin Graft Survival

Production by Cultured Spleen Cells of Inflammatory Substances and Other Lymphokines that Mediate Delayed‐Type Hypersensitivity in Mice

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