Deleterious Interaction Between Honeybees (Apis mellifera) and its Microsporidian Intracellular Parasite Nosema ceranae Was Mitigated by Administrating Either Endogenous or Allochthonous Gut Microbiota Strains

Khoury, Sarah El; Rousseau, Andrée; Lecoeur, Alexandre; Cheaib, Bachar; Bouslama, Sidki; Mercier, Pierre Luc; Demey, V.; Castex, Mathieu; Giovenazzo, Pierre; Derôme, Nicolas

doi:10.3389/fevo.2018.00058

Cited by 54 publications

(49 citation statements)

References 110 publications

(142 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because pollen is the main polysaccharide source in the honey bee diet, we assumed that pollen feeding and V. ceranae infections altered the bee microbiota in those studies. Similar results were obtained when indigenous gut bacteria were gavaged into bees with microsporidian infection [42], but neither did this study evaluate microbiota alterations. Feeding antibiotics and V. ceranae to bees showed the opposite results in that mortality increased [43].…”

Section: Discussionsupporting

confidence: 66%

Vairimorpha Ceranae Alters Honey Bee Microbiota Composition and Sustain the Survival of Adult Honey Bees

Zhang¹,

Su²,

Wang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Vairimorpha (Nosema) ceranae is the most common eukaryotic gut pathogen in honey bees, Apis mellifera. Infection is typically chronic but may result in mortality. Additional factors may be involved in mortality, including the honey bee gut microbiota. Previous studies of V. ceranae and gut microbiota identified positive associations between core bacteria and V. ceranae infection. These possibly synergistic or mutualistic associations are often disregarded because some core bacteria act as probiotic symbionts. Methods: To clarify the effects caused by the positive associations, we added isomaltooligosaccharide (IMO), a prebiotic also found in honey, to alter the interactions between V. ceranae and gut bacteria. Mortality and sugar consumption of the caged bees were monitored. Infection intensities and gut bacteria were examed after 12 days post inoculation, the plateau phase of infection. The gut bacteria were evaluated using both qPCR and 16S rDNA sequencing.Results: We confirmed that V. ceranae infections alone significantly enhance several core bacterial populations, including Bifidobacterium spp., Snodgrassella alvi, and Gilliamella apicola in the honey bee hindgut microbiota. Moreover, the qPCR results suggested that V. ceranae infected bees had significantly higher bacterial microbiota populations. In addition to the enhanced core bacteria, Commensalibacter and Bartonella were significantly increased in the fecal microbiome. Infected bees fed IMO had significantly higher V. ceranae spore counts but lower mortality; however, infected bees fed IMO did not have significant changes in gut bacteria populations compared to those fed only sucrose, but feeding IMO further reduced the fecal microbiome alpha-diversity. Conclusions: The microbiota alterations caused by infection were similar to the microbiota differences found between summer bees and winter bees, the latter of which have longer lifespans, and feeding IMO increased this similarity. Our results indicated that the interactions between gut bacteria and V. ceranae not only enhanced both the pathogen and bacteria populations but also sustained the host survival. This mutualistic interaction potentially enhances disease transmission and avoids social immune responses of the honey bee hosts.

show abstract

Section: Discussionsupporting

confidence: 66%

Vairimorpha Ceranae Alters Honey Bee Microbiota Composition and Sustain the Survival of Adult Honey Bees

Zhang¹,

Su²,

Wang³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The selection and availability of new selected bacteria with good functional characters and with antagonistic activity against P. larvae always opens up interesting perspectives for new biocontrol strategies of diseases such as the AFB. Some researchers have highlighted the effectiveness of LAB in controlling this disease [45][46][47][76][77][78]139,140], and other researches have shown that effectiveness is not always certain in the hive [141,142] or that supplementation of honeybee diet, with improper probiotics, can be harmful to honeybees [143,144]. The functional properties, shown in vitro using L. plantarum strains, do not result axiomatically in health benefits for honeybee colonies.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity against Paenibacillus larvae and Functional Properties of Lactiplantibacillus plantarum Strains: Potential Benefits for Honeybee Health

et al. 2020

View full text Add to dashboard Cite

Paenibacillus larvae is the causative agent of American foulbrood (AFB), a severe bacterial disease that affects larvae of honeybees. The present study evaluated, in vitro, antimicrobial activity of sixty-one Lactiplantibacillus plantarum strains, against P. larvae ATCC 9545. Five strains (P8, P25, P86, P95 and P100) that showed the greatest antagonism against P. larvae ATCC 9545 were selected for further physiological and biochemical characterizations. In particular, the hydrophobicity, auto-aggregation, exopolysaccharides production, osmotic tolerance, enzymatic activity and carbohydrate assimilation patterns were evaluated. The five L. plantarum selected strains showed suitable physical and biochemical properties for their use as probiotics in the honeybee diet. The selection and availability of new selected bacteria with good functional characteristics and with antagonistic activity against P. larvae opens up interesting perspectives for new biocontrol strategies of diseases such as AFB.

show abstract

“…This probiotic treatment has completely restored the survival rates of infected honeybees in Exp.1 suggesting that PA treatment efficiency is enhanced when it is administered before the infection. The survival rate improvement by PA has already been shown in both curative and prophylactic administrations with a survival probability enhance of 20-30% in honeybees treated with a commercial product containing the same strain (El Khoury et al, 2018). Similar effects were highlighted with the prophylactic administration of Biogen-N, a probiotic formulation containing PA among other strains (Kaznowski et al, 2005) and in other species like piglets (Di Giancamillo et al, 2008;Dowarah et al, 2018), red tilapia (Ferguson et al, 2010), rainbow trout (Merrifield et al, 2010) or chickens (Jazi et al, 2018).…”

Section: Discussionmentioning

confidence: 68%

“…Administration of selected microorganisms, in this dysbiotic context, could thus represent beneficial microbes that could be used as probiotics. If probiotics are commonly used in vertebrates (Crotti et al, 2012), few studies have focused on their impact on honeybee health (Audisio et al, 2015;Baffoni et al, 2016;El Khoury et al, 2018;Gaggìa et al, 2018;Ptaszyńska et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

A Pediococcus strain to rescue honeybees by decreasing Nosema ceranae- and pesticide-induced adverse effects

Peghaire

Moné

Delbac

et al. 2020

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

show abstract

Deleterious Interaction Between Honeybees (Apis mellifera) and its Microsporidian Intracellular Parasite Nosema ceranae Was Mitigated by Administrating Either Endogenous or Allochthonous Gut Microbiota Strains

Cited by 54 publications

References 110 publications

Vairimorpha Ceranae Alters Honey Bee Microbiota Composition and Sustain the Survival of Adult Honey Bees

Vairimorpha Ceranae Alters Honey Bee Microbiota Composition and Sustain the Survival of Adult Honey Bees

Antimicrobial Activity against Paenibacillus larvae and Functional Properties of Lactiplantibacillus plantarum Strains: Potential Benefits for Honeybee Health

A Pediococcus strain to rescue honeybees by decreasing Nosema ceranae- and pesticide-induced adverse effects

Contact Info

Product

Resources

About