Deletion of TerD-domain-encoding genes: effect on Streptomyces coelicolor development

Abstract

TerD-domain-encoding genes (tdd genes) are highly represented in the Streptomyces coelicolor genome. One of these, the tdd8 gene, was recently shown to have a crucial influence on growth, differentiation, and spore development of this filamentous bacterium. The investigation of the potential role of tdd genes has been extended here to tdd7 (SCO2367) and tdd13 (SCO4277). Both genes are highly expressed in bacteria grown in liquid-rich medium (tryptic soy broth). However, the deletion of these genes in S. coelicolor showed contrasting effects regarding developmental patterns, sporulation, and antibiotic production. Deletion of the tdd7 gene induced a reduction of growth in liquid medium, wrinkling of the mycelium on solid medium, and poor spore and actinorhodin production. On the other hand, deletion of the tdd13 gene did not significantly affect growth in liquid medium but induced a small colony phenotype on solid medium with abundant sporulation and overproduction of undecylprodigiosin. Although their exact functions remain undefined, the present data suggest a major involvement of TerD proteins in the proper development of S. coelicolor.

Quantitative Proteomics Analysis Confirmed Oxidative Metabolism Predominates inStreptomyces coelicolorversus Glycolytic Metabolism inStreptomyces lividans

“…It is noteworthy that the TerD-like protein SCO2368, a protein known to down-regulate the expression of genes of the DosR-like regulon, was more abundant in S. lividans than in S. coelicolor . The function of the other TerD proteins is not clearly known, but some studies suggest that these proteins might play a role in growth slowdown under adverse conditions to improve cell survival. , In addition, a proteomics study of S. viridochromogenes showed the up-regulation of tellurium resistance proteins in response to oxidative stress . A protein of the universal stress protein (USP) family (SCO7299) was also found to be 5.7 times more abundant in S. lividans than in S. coelicolor .…”

AterDDomain-Encoding Gene (SCO2368) Is Involved in Calcium Homeostasis and Participates in Calcium Regulation of a DosR-Like Regulon in Streptomyces coelicolor

“…For instance, it was demonstrated that deletion or overexpression of tdd7, tdd8, or tdd13 affected morphological differentiation or spore production in S. coelicolor (11,13). A strain with a deletion in tdd8 (SCO2368) produced long chains of short spores with a dense spore wall, while a strain overexpressing tdd8 produced very few spores of irregular shapes and sizes (11).…”