Deletion of the
            <i>MBP1</i>
            Gene, Involved in the Cell Cycle, Affects Respiration and Pseudohyphal Differentiation in Saccharomyces cerevisiae

Chen, Xiaoling; Zhang, Lu; Chen, Ying; Wu, Renzhi; Luo, Ziqiang; Lü, Qi; Guan, Ni; Dong, Chen

doi:10.1128/spectrum.00088-21

Cited by 5 publications

(1 citation statement)

References 65 publications

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“…Enolase (ENO) as a metabolic enzyme implicated in pyruvate synthesis, maintaining the level of intracellular ATP and meeting the energy required for life activities. The c‐Myc promoter binding protein 1 (MBP‐1), as the splice variant of ENO1 (OMIM: 172430), enables DNA‐binding transcription activator activity and positively modulates transcription involved in the G1/S transition of mitotic cell cycle (Chen et al, 2021 ). ENO is widely present on the cytoplasm, nucleus, and the cell membrane (Caturegli et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Alpha‐enolase 1 knockdown facilitates the proliferation and invasion of villous trophoblasts by upregulating COX‐2

Tang

Pan

Tang

et al. 2023

Molec Gen & Gen Med

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BackgroundEnolase 1 (ENO1) is a metabolic enzyme which participates in pyruvate synthesis and ATP production in cells. Previously, differential expression of ENO1 was discovered in villous tissues between recurrent miscarriage and induced abortion. This study was designed to explore whether ENO1 influences the proliferation and invasion of villous trophoblasts and the related molecular mechanisms.MethodsFirst, ENO1 expression in placental villus tissues collected from recurrent miscarriage (RM) patients and women for induced abortion as well as in trophoblast‐derived cell lines was detected by RT‐qPCR and western blotting. ENO1 localization and expression in villus tissues were further confirmed through immunohistochemistry staining. Then, the effects of ENO1 downregulation on trophoblast Bewo cell proliferation, migration, invasion, and epithelial‐mesenchymal transition (EMT) process were evaluated by CCK‐8 assay, transwell assay, and western blotting. As for the regulatory mechanism of ENO1, the expression of COX‐2, c‐Myc and cyclin D1 in Bewo cells after ENO1 knockdown was finally evaluated by RT‐qPCR and western blotting.ResultsENO1 was mainly localized in the cytoplasm, with very small amounts in the nucleus of trophoblast cells. ENO1 expression in the villi tissues of RM patients was significantly increased, when compared with the villous tissues of healthy controls. Furthermore, Bewo cells, a trophoblast cell line with relatively higher expression of ENO1, was used to downregulate the ENO1 expression by ENO1‐siRNA transfection. ENO1 knockdown significantly facilitated Bewo cell growth, EMT process, migration, and invasion. ENO1 silencing markedly elevated COX‐2, c‐Myc, and cyclin D1 expression.ConclusionENO1 may participate in the development of RM via suppressing the growth and invasion of villous trophoblasts via reducing the expression of COX‐2, c‐Myc, and cyclin D1.

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Section: Introductionmentioning

confidence: 99%

Alpha‐enolase 1 knockdown facilitates the proliferation and invasion of villous trophoblasts by upregulating COX‐2

Tang

Pan

Tang

et al. 2023

Molec Gen & Gen Med

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Putative APSES family transcription factor mbp1 plays an essential role in regulating cell wall synthesis in the agaricomycete Pleurotus ostreatus

Kojima,

Kawauchi,

Otsuka

et al. 2024

Fungal Genetics and Biology

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Role of putative APSES family transcription factor Swi6 in cell wall synthesis regulation in the agaricomycete Pleurotus ostreatus

Kojima,

Izumi,

Kawauchi

et al. 2025

Fungal Biology

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Deletion of the MBP1 Gene, Involved in the Cell Cycle, Affects Respiration and Pseudohyphal Differentiation in Saccharomyces cerevisiae

Abstract: Saccharomyces cerevisiae is an especially favorable organism used for ethanol production. However, inhibitors and high osmolarity conferred by fermentation broth, and high concentrations of ethanol as fermentation runs to completion, affect cell growth and ethanol production.

Cited by 5 publications

References 65 publications

Alpha‐enolase 1 knockdown facilitates the proliferation and invasion of villous trophoblasts by upregulating COX‐2

Alpha‐enolase 1 knockdown facilitates the proliferation and invasion of villous trophoblasts by upregulating COX‐2

Putative APSES family transcription factor mbp1 plays an essential role in regulating cell wall synthesis in the agaricomycete Pleurotus ostreatus

Role of putative APSES family transcription factor Swi6 in cell wall synthesis regulation in the agaricomycete Pleurotus ostreatus

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