Deletion of the msdS/AfmsdC gene induces abnormal polarity and septation in Aspergillus fumigatus

Li, Yanjie; Zhang, Lei; Wang, Depeng; Zhou, Hui; Ouyang, Hongwei; Ming, Jia; Jin, Cheng

doi:10.1099/mic.0.2008/017525-0

Cited by 23 publications

(22 citation statements)

References 47 publications

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“…While genes involved in hydrolysing β‐1,3 glucan showed enhanced expression in the mutant, the α‐1,3 glucanase gene (Gastebois et al ., 2009) required for modification of α‐1,3 glucan displayed reduction in the mutant under non‐cell wall stress conditions. Other genes that showed downregulation in the Δ mpkA strain were rodB , a hydrophobin playing a role in maintaining the structure of the conidial cell wall (Paris et al ., 2003a), mannosidase msdS (Li et al ., 2008) and dimethylallyl tryptophan synthase. The latter two are both related to N ‐glycan processing and thus affect the organization of the fungal cell wall (Fig.…”

Section: Resultsmentioning

confidence: 99%

The MAP kinase MpkA controls cell wall integrity, oxidative stress response, gliotoxin production and iron adaptation in Aspergillus fumigatus

Jain

Valiante

Remme

et al. 2011

Molecular Microbiology

126

124

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The saprophytic fungus Aspergillus fumigatus is the most important air-borne fungal pathogen. The cell wall of A. fumigatus has been studied intensively as a potential target for development of effective antifungal agents. A major role in maintaining cell wall integrity is played by the mitogen-activated protein kinase (MAPK) MpkA. To gain a comprehensive insight into this central signal transduction pathway, we performed a transcriptome analysis of the ΔmpkA mutant under standard and cell wall stress conditions. Besides genes involved in cell wall remodelling, protection against ROS and secondary metabolism such as gliotoxin, pyomelanin and pseurotin A, also genes involved in siderophore biosynthesis were regulated by MpkA. Consistently, northern and western blot analyses indicated that iron starvation triggers phosphorylation and thus activation of MpkA. Furthermore, localization studies indicated that MpkA accumulates in the nucleus under iron depletion. Hence, we report the first connection between a MAPK pathway and siderophore biosynthesis. The measurement of amino acid pools and of the pools of polyamines indicated that arginine was continuously converted into ornithine to fuel the siderophore pool in the ΔmpkA mutant strain. Based on our data, we propose that MpkA fine-tunes the balance between stress response and energy consuming cellular processes.

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Section: Resultsmentioning

confidence: 99%

The MAP kinase MpkA controls cell wall integrity, oxidative stress response, gliotoxin production and iron adaptation in Aspergillus fumigatus

Jain

Valiante

Remme

et al. 2011

Molecular Microbiology

126

124

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“…Morphological analysis reveals abnormal polarity and septation. However, deletion of the msdS has no effect on fungal growth and virulence [97]. …”

Section: Biosynthesis and Function Of N-glycosylation In A Fumigamentioning

confidence: 99%

Protein Glycosylation inAspergillus fumigatusIs Essential for Cell Wall Synthesis and Serves as a Promising Model of Multicellular Eukaryotic Development

Jin

2012

International Journal of Microbiology

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Glycosylation is a conserved posttranslational modification that is found in all eukaryotes, which helps generate proteins with multiple functions. Our knowledge of glycosylation mainly comes from the investigation of the yeast Saccharomyces cerevisiae and mammalian cells. However, during the last decade, glycosylation in the human pathogenic mold Aspergillus fumigatus has drawn significant attention. It has been revealed that glycosylation in A. fumigatus is crucial for its growth, cell wall synthesis, and development and that the process is more complicated than that found in the budding yeast S. cerevisiae. The present paper implies that the investigation of glycosylation in A. fumigatus is not only vital for elucidating the mechanism of fungal cell wall synthesis, which will benefit the design of new antifungal therapies, but also helps to understand the role of protein glycosylation in the development of multicellular eukaryotes. This paper describes the advances in functional analysis of protein glycosylation in A. fumigatus.

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“…␣-Mannosidases have an important function in the processing of N-glycans, and the 1,2-␣-mannosidase MsdS in A. fumigatus plays an important role in morphogenesis. The deletion of msdS results in defective N-glycan processing, and a ⌬msdS mutant displays abnormal germination, hyphal growth, and conidiation caused by defects in polarity and septation (328). Similarly, the ␣-glucosidase Cwh41, which is also involved in A. fumigatus N-glycan processing, is required for proper polar growth, elongation, and septation during hyphal growth (646).…”

Section: Major Morphogenetic Signaling Cascadesmentioning

confidence: 99%

Regulatory Circuitry Governing Fungal Development, Drug Resistance, and Disease

Shapiro

Robbins

Cowen

2011

Microbiol Mol Biol Rev

501

547

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SUMMARY Pathogenic fungi have become a leading cause of human mortality due to the increasing frequency of fungal infections in immunocompromised populations and the limited armamentarium of clinically useful antifungal drugs. Candida albicans , Cryptococcus neoformans , and Aspergillus fumigatus are the leading causes of opportunistic fungal infections. In these diverse pathogenic fungi, complex signal transduction cascades are critical for sensing environmental changes and mediating appropriate cellular responses. For C. albicans , several environmental cues regulate a morphogenetic switch from yeast to filamentous growth, a reversible transition important for virulence. Many of the signaling cascades regulating morphogenesis are also required for cells to adapt and survive the cellular stresses imposed by antifungal drugs. Many of these signaling networks are conserved in C. neoformans and A. fumigatus , which undergo distinct morphogenetic programs during specific phases of their life cycles. Furthermore, the key mechanisms of fungal drug resistance, including alterations of the drug target, overexpression of drug efflux transporters, and alteration of cellular stress responses, are conserved between these species. This review focuses on the circuitry regulating fungal morphogenesis and drug resistance and the impact of these pathways on virulence. Although the three human-pathogenic fungi highlighted in this review are those most frequently encountered in the clinic, they represent a minute fraction of fungal diversity. Exploration of the conservation and divergence of core signal transduction pathways across C. albicans , C. neoformans , and A. fumigatus provides a foundation for the study of a broader diversity of pathogenic fungi and a platform for the development of new therapeutic strategies for fungal disease.

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Deletion of the msdS/AfmsdC gene induces abnormal polarity and septation in Aspergillus fumigatus

Cited by 23 publications

References 47 publications

The MAP kinase MpkA controls cell wall integrity, oxidative stress response, gliotoxin production and iron adaptation in Aspergillus fumigatus

The MAP kinase MpkA controls cell wall integrity, oxidative stress response, gliotoxin production and iron adaptation in Aspergillus fumigatus

Protein Glycosylation inAspergillus fumigatusIs Essential for Cell Wall Synthesis and Serves as a Promising Model of Multicellular Eukaryotic Development

Regulatory Circuitry Governing Fungal Development, Drug Resistance, and Disease

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