Delftia acidovorans secretes substances that inhibit the growth of Staphylococcus epidermidis through TCA cycle-triggered ROS production

Abstract

The proportion of Staphylococcus aureus in the skin microbiome is associated with the severity of inflammation in the skin disease atopic dermatitis. Staphylococcus epidermidis, a commensal skin bacterium, inhibits the growth of S. aureus in the skin. Therefore, the balance between S. epidermidis and S. aureus in the skin microbiome is important for maintaining healthy skin. In the present study, we demonstrated that the heat-treated culture supernatant of Delftia acidovorans, a member of the skin microbiome, inhibits the growth of S. epidermidis, but not that of S. aureus. Comprehensive gene expression analysis by RNA sequencing revealed that culture supernatant of D. acidovorans increased the expression of genes related to glycolysis and the tricarboxylic acid cycle (TCA) cycle in S. epidermidis. Malonate, an inhibitor of succinate dehydrogenase in the TCA cycle, suppressed the inhibitory effect of the heat-treated culture supernatant of D. acidovorans on the growth of S. epidermidis. Reactive oxygen species production in S. epidermidis was induced by the heat-treated culture supernatant of D. acidovorans and suppressed by malonate. Further, the inhibitory effect of the heat-treated culture supernatant of D. acidovorans on the growth of S. epidermidis was suppressed by N-acetyl-L-cysteine, a free radical scavenger. These findings suggest that heat-resistant substances secreted by D. acidovorans inhibit the growth of S. epidermidis by inducing the production of reactive oxygen species via the TCA cycle.

Thermal Therapy Modulation of the Psoriasis-Associated Skin and Gut Microbiome

“…The genus Delftia , increased after balneotherapy, is instead known to degrade organic pollutants and includes both environmental and opportunistic pathogenic strains [ 59 – 63 ]. Interestingly, Delftia was previously associated with a reduction of S. epidermidis and a consequent increase in S. aureus on the skin of patients with atopic dermatitis [ 64 ], a trend also observed in our cohort. These findings suggest that balneotherapy influences and possibly shifts skin microbiome relative abundances toward a more healthy-like composition.…”

Unveiling the Microbiome Diversity in Telenomus (Hymenoptera: Scelionidae) Parasitoid Wasps

“…Particularly, Delftia species have been related to the ability to degrade organophosphates [31], and some strains are associated with degradation routes for di-n-butylphthalate (DBP, industrial pollutant) [32]. In the same way, these bacteria can produce bacteriocins against some bacterial and fungal pathogens [33][34][35][36]. This genus has been found in the coffee insect pest Hypothenemus hampei Ferrari (Coleoptera: Curculion-idae) [37] and was isolated from the gut of chlorpyrifos ethyl-resistant insect larvae [38].…”