Delignification of disposable wooden chopsticks waste for fermentative hydrogen production by an enriched culture from a hot spring

Phummala, Kanthima; Imai, Tsuyoshi; Reungsang, Alissara; Chairattanamanokorn, Prapaipid; Sekine, Masahiko; Higuchi, Takaya; Yamamoto, Kōichi; Kanno, Ariyo

doi:10.1016/s1001-0742(13)60612-9

Cited by 25 publications

(8 citation statements)

References 26 publications

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“…The assessment of microbial background by PCR-DGGE based on the 16S rDNA analysis is well-accepted in hydrogen production processes [19,20]. In this study the denaturing gradient concentration of 40 % to 60 % was used to separate the band pattern of the different microbial community.…”

Section: Microbial Community Profilingmentioning

confidence: 99%

Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains

Kumar

Bakonyi

Sivagurunathan

et al. 2015

Journal of Bioscience and Bioengineering

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In this work biohydrogen generation and its improvement possibilities from beverage industrial wastewater were sought. Firstly, mesophilic hydrogen fermentations were conducted in batch vials by applying heat-treated (80 o C, 30 min) sludge and liquid (LB-grown) cultures of Escherichia coli (XL1-Blue)/Enterobacter cloacae (DSM 16657) strains for bioaugmentation purposes. The results showed that there was a remarkable increase in hydrogen production capacities when facultative anaerobes were added in the form of inoculum. Furthermore, experiments were carried out in order to reveal whether the increment occurred either due to the efficient contribution of the facultative anaerobic microorganisms or the culture ingredients (in particular yeast extract and tryptone) supplied when the bacterial suspensions (LB media-based inocula) were mixed with the sludge. The outcome of these tests was that both the applied nitrogen sources and the bacteria (E. coli) could individually enhance hydrogen formation.Nevertheless, the highest increase took place when they were used together. Finally, the optimal initial wastewater concentration was determined as 5 g/L.

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Section: Microbial Community Profilingmentioning

confidence: 99%

Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains

Kumar

Bakonyi

Sivagurunathan

et al. 2015

Journal of Bioscience and Bioengineering

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“…Enzymatic hydrolysate and fermented medium were collected to analyze glucose, xylose and ethanol. The concentrations of glucose, xylose and ethanol were determined by HPLC (Phummala et al, 2014). Reducing sugar was analyzed by the dinitrosalicylic acid (DNS) method.…”

Section: Methodsmentioning

confidence: 99%

“…Biogas volume was measured periodically using a wet glass syringe. The content of biogas in the headspace, including H 2 , N 2 , CH 4 and CO 2 , was analyzed using a GC-8APT gas chromatograph, equipped with a thermal conductivity detector (TCD) (Shimadzu, Kyoto, Japan) (Phummala et al, 2014). The cumulative H 2 production data of each test were fitted to a modified Gompertz equation (Khanal et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

“…Ten percent (w/v dry mass) of DWC was carried out with 2% NaOH at 100°C for 30 minutes (Phummala et al, 2014). After that, pretreated DWC (cellulose fractions) were sieved through a muslin cloth, and washed with tap water until neutral, dried at 45°C, and stored at room temperature in a sealed container.…”

Section: Alkaline Pretreatmentmentioning

confidence: 99%

“…An anaerobic mixed culture was enriched from a hot spring. Optimal pH and temperature were investigated at 7.0 and 50°C, respectively (Phummala et al, 2014). The mixed culture was used as an inoculum for fermentative H 2 production.…”

Section: Hydrogen Productionmentioning

confidence: 99%

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Optimization of Enzymatic Hydrolysis for Pretreated Wood Waste by Response Surface Methodology in Fermentative Hydrogen Production

Phummala

Imai

Reungsang

et al. 2015

J. of Wat. & Envir. Tech.

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Enzymatic hydrolysis is an essential step in the fermentative H 2 production process of lignocellulosic biomass to convert cellulose and hemicellulose into fermentable sugars. The waste from disposable wooden chopsticks (DWC) was represented as wood waste and investigated in this study. In order to optimize the condition of the enzymatic hydrolysis of alkaline pretreated DWC, response surface methodology is an efficient experimental tool used to determine the optimal conditions of numerous variables. In the present study, cellulase dosage, β-glucosidase dosage, Tween 80, and hydrolysis time, were found to have a significant effect on enzymatic hydrolysis based on the Plackett-Burman design. These factors were subsequently investigated on the optimal levels by a central composite design, which was determined at 36 FPU/g pretreated DWC of cellulase, 53 CBU/g pretreated DWC of β-glucosidase, and 0.4 g/g pretreated DWC of Tween 80 for 105 h. Under optimal conditions, glucose and reducing sugar yields were 121.7 and 435.8 mg/g pretreated DWC, respectively. Furthermore, enzymatic hydrolysate was applied as a substrate for fermentative H 2 production and obtained a yield of 27 mL H 2 /g pretreated DWC by an anaerobic mixed culture.

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Pre‐Treatment of Lignocellulose for the Production of Biofuels

Ghosh¹,

Bhattacharya

Mukhopadhyay³

2018

Principles and Applications of Fermentation Technology

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Delignification of disposable wooden chopsticks waste for fermentative hydrogen production by an enriched culture from a hot spring

Cited by 25 publications

References 26 publications

Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains

Enhanced biohydrogen production from beverage industrial wastewater using external nitrogen sources and bioaugmentation with facultative anaerobic strains

Optimization of Enzymatic Hydrolysis for Pretreated Wood Waste by Response Surface Methodology in Fermentative Hydrogen Production

Pre‐Treatment of Lignocellulose for the Production of Biofuels

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