Delignification Process of Agro-Industrial Wastes an Alternative to Obtain Fermentable Carbohydrates for Producing Fuel

Sánchez, Óscar J.; Sierra, R.; Alméciga-Díaz, Carlos J.

doi:10.5772/22381

Cited by 32 publications

(25 citation statements)

References 204 publications

(214 reference statements)

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“…The saponification of the ester bonds in the lignin results in the breakage of the bonds between lignin and carbohydrates and leads to the release of lignin into the solution [13]. Both hemicellulose and lignin would be affected by the lime treatment and cellulose would be affected the least [31]. The lime treatment of the autohydrolysed lignocellulosic material can be applied to extract the lignin fraction leaving a cellulose rich residue [18].…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of steam-assisted treatments of biomass components and sweet sorghum bagasse

et al. 2015

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A process originally used for the fractionation of sweet sorghum bagasse powder was evaluated using biomass components such as cellulose, xylan and lignin. The autohydrolysis of the substrates was conducted at 121 §0.5 C with isothermal treatment time of 90 min. The lime treatment of the autohydrolysed substrates was conducted at 121 §0.5 C for 106 min with 0.1 g Ca(OH) 2 dosage per gram of the substrates. The responses from the treatment of the biomass components were used to evaluate the responses observed during the treatment of the sweet sorghum bagasse. Carbon dioxide gas at a flow rate of 17 mL Ã min ¡1 was used for the precipitation of lignin and lime from the liquid obtained from the lime treatment process. The process responses were also compared and confirmed using the Fourier transform infrared spectrophotometry and the differential scanning calorimetry of the sweet sorghum bagasse and the treated and untreated biomass components.

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Section: Introductionmentioning

confidence: 99%

Comparative evaluation of steam-assisted treatments of biomass components and sweet sorghum bagasse

et al. 2015

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“…Conversely, lignin biodegradation occurs at a lower rate than plant cell wall polysaccharides [2]. Certain fungi, mostly belonging to Basidiomycetes, such as white- and brown-rot fungi, are known to be able to degrade lignin from lignocellulosic biomass [3]. The extracellular enzymatic system responsible for lignin degradation consists of lignin peroxidase (LiP, E.C.…”

Section: Introductionmentioning

confidence: 99%

“…1.11.1.14), manganese-dependent peroxidase (MnP, E.C. 1.11.1.13), and laccase (para-benzene-diol: oxygen oxidoreductase, EC 1.10.3.2I) [2,3]. While LiP catalyzes the oxidation of non-phenolic aromatic compounds such as veratryl alcohol, and MnP mainly oxidizes phenolic compounds [2], laccase catalyzes the oxidation of phenolic substrates with the concomitant reduction of oxygen to water [4].…”

Section: Introductionmentioning

confidence: 99%

Production of Trametes pubescens Laccase under Submerged and Semi-Solid Culture Conditions on Agro-Industrial Wastes

et al. 2013

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Laccases are copper-containing enzymes involved in the degradation of lignocellulosic materials and used in the treatment of phenol-containing wastewater. In this study we investigated the effect of culture conditions, i.e. submerged or semi-solid, and copper supplementation on laccase production by Trametes pubescens grown on coffee husk, soybean pod husk, or cedar sawdust. The highest specific laccase activity was achieved when the culture was conducted under submerged conditions supplemented with copper (5 mM), and using coffee husk as substrate. The crude extracts presented two laccase isoforms with molecular mass of 120 (Lac1) and 60 kDa (Lac2). Regardless of the substrate, enzymatic crude extract and purified fractions behaved similarly at different temperatures and pHs, most of them presented the maximum activity at 55 °C and a pH range between 2 and 3. In addition, they showed similar stability and electro-chemical properties. At optimal culture conditions laccase activity was 7.69±0.28 U mg-1 of protein for the crude extract, and 0.08±0.001 and 2.86±0.05 U mg-1 of protein for Lac1 and Lac2, respectively. In summary, these results show the potential of coffee husk as an important and economical growth medium to produce laccase, offering a new alternative use for this common agro-industrial byproduct.

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“…The rigorous and chemically-efficient two-stage chemical Specifically, for alkaline pretreatments, the lignin degradation is achieved by cleavage of aryl ether bonds, Caliphatic-O-Caromatic or Caromatic-O-Caromatic resulting in ring opening, degradation, and solubilization [37]. Pretreatment effluents and lignin that are highly soluble in solution or result in low molecular weight fractions of lignin are more easily utilized by the microorganisms.…”

Section: Lignocellulosic Biomass Pretreatment Strategiesmentioning

confidence: 99%

A Review on The Bioconversion of Lignin to Microbial Lipid with Oleaginous Rhodococcus opacus

Mahan¹,

Le²,

Yuan³

et al. 2017

J Biotechnol Biomater

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Rhodococcus opacus produces intracellular lipids from the biodegradation of lignocellulosic biomass. These lipids can be used to produce biofuels that could potentially replace petroleum-derived chemicals. Current studies are focusing on deconstructing lignin through efficient and cost-effective pretreatment methods and improving microbial lipid titers. R. opacus can reach high levels of oleaginicity (>80%) when grown on glucose and other aromatic model compounds but intracellular lipid production is much lower on complex recalcitrant lignin substrates. This review will discuss recent advances in studying R. opacus lignin degradation by exploring different pretreatment methods, increasing lignin solubility, enriching for low molecular weight lignin compounds and laccase supplementation.

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Delignification Process of Agro-Industrial Wastes an Alternative to Obtain Fermentable Carbohydrates for Producing Fuel

Cited by 32 publications

References 204 publications

Comparative evaluation of steam-assisted treatments of biomass components and sweet sorghum bagasse

Comparative evaluation of steam-assisted treatments of biomass components and sweet sorghum bagasse

Production of Trametes pubescens Laccase under Submerged and Semi-Solid Culture Conditions on Agro-Industrial Wastes

A Review on The Bioconversion of Lignin to Microbial Lipid with Oleaginous Rhodococcus opacus

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