Delineation of a frequency-organized region isolated from the mouse primary auditory cortex

Tsukano, Hiroaki; Horie, Minoru; Bo, Takeshi; Uchimura, Arikuni; Hishida, Ryuichi; Kudoh, Masaharu; Takahashi, Kuniyuki; Takebayashi, Hirohide; Shibuki, Katsuei

doi:10.1152/jn.00932.2014

Cited by 66 publications

(116 citation statements)

References 84 publications

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“…However, the results of the current study clearly shows the presence of dense connections between A2 and the amygdala, while other auditory fields have slight or no connections with the amygdala. Moreover, previous studies have reported that A2 neurons have long latencies (Kubota et al, 2008;Guo et al, 2012;Tsukano et al, 2015), wide bandwidth (Issa et al, 2014;Ohga et al, 2018), and a less-ordered tonotopic arrangement (Issa et al, 2014;Ohga et al, 2018). These data confirm that mouse A2 has properties of a higher-order region, and also support the idea that auditory cortical subregions are functionally specialized in mice (Honma et al, 2013;Baba et al, 2016;Issa et al, 2017).…”

Section: Discussionsupporting

confidence: 85%

Reciprocal connectivity between secondary auditory cortical field and amygdala in mice

Tsukano

Hou

Horie

et al. 2019

Preprint

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Recent studies have examined the feedback pathway from the amygdala to the auditory cortex in conjunction with the feedforward pathway from the auditory cortex to the amygdala. However, these connections have not been fully characterized. Here, to visualize the comprehensive connectivity between the auditory cortex and amygdala, we injected cholera toxin subunit b (CTB), a bidirectional tracer, into multiple subfields in the mouse auditory cortex after identifying the location of these subfields using flavoprotein fluorescence imaging. After injecting CTB into the secondary auditory field (A2), we found densely innervated CTB-positive axon terminals that were mainly located in the lateral amygdala (La), and slight innervations in other divisions such as the basal amygdala. Moreover, we found a large number of retrogradely-stained CTB-positive neurons in La after injecting CTB into A2. When injecting CTB into the primary auditory cortex (A1), a small number of CTB-positive neurons and axons were visualized in the amygdala. Finally, we found a near complete absence of connections between the other auditory cortical fields and the amygdala. These data suggest that reciprocal connections between A2 and La are main conduits for communication between the auditory cortex and amygdala in mice.

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Section: Discussionsupporting

confidence: 85%

Reciprocal connectivity between secondary auditory cortical field and amygdala in mice

Tsukano

Hou

Horie

et al. 2019

Preprint

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“…This region possesses a weak tonotopic organization (Issa et al, 2014; Tsukano et al, 2015) and, based on our widefield imaging results (Figure 2D and 2G), should be strongly responsive to FM sweeps as well. Indeed, neurons in this field are tuned to high frequencies (Figure 5G) and, more interestingly, respond heterogeneously to sweep rate (Figure 5H).…”

Section: Resultsmentioning

confidence: 68%

“…As for the location of CSR, a nearby “dorsoanterior field” (DA) was found to be responsive to reversals in FM sweep direction (Tsukano et al, 2016, 2015). It remains to be determined if DA and CSR are related since FM sweeps used in those studies included direction reversals and superimposed sweeps and were at their fastest about 5 oct/s, slower than any of the FM stimuli used in this current study.…”

Section: Discussionmentioning

confidence: 99%

“…In other species, certain regions prefer faster FM sweeps, such as a ventral region at the border of the primary auditory cortex (AI) and the secondary auditory cortex (AII) in cats (Mendelson et al, 1993), various lateral belt areas in rhesus monkeys (Tian and Rauschecker, 2004), and the anterior auditory field (AAF) in mice (Trujillo et al, 2011). In addition, regions of the ultrasonic field (UF) have been noted to prefer frequency modulations (Stiebler et al, 1997) and are selective for reversals in FM direction (Honma et al, 2013; Tsukano et al, 2016, 2015). While these studies highlight the potential specialization of cortical regions for FM sweep processing, a more comprehensive mapping of FM selectivity, which would facilitate the search for dedicated FM areas, is lacking.…”

Section: Introductionmentioning

confidence: 99%

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Multiscale mapping of frequency sweep rate in mouse auditory cortex

et al. 2017

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Functional organization is a key feature of the neocortex that often guides studies of sensory processing, development, and plasticity. Tonotopy, which arises from the transduction properties of the cochlea, is the most widely studied organizational feature in auditory cortex; however, in order to process complex sounds, cortical regions are likely specialized for higher order features. Here, motivated by the prevalence of frequency modulations in mouse ultrasonic vocalizations and aided by the use of a multiscale imaging approach, we uncover a functional organization across the extent of auditory cortex for the rate of frequency modulated (FM) sweeps. In particular, using two-photon Ca2+ imaging of layer 2/3 neurons, we identify a tone-insensitive region at the border of AI and AAF. This central sweep region behaves fundamentally differently from nearby neurons in AI and AII, responding preferentially to fast FM sweeps but not to tones or bandlimited noise. Together these findings define a second dimension of organization in the mouse auditory cortex for sweep rate complementary to that of tone frequency.

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“…In contrast, stimuli with higher frequencies evoked cortical responses in the red colored area. According to recent studies investigating the organization of mouse auditory cortex 30,32,33 , the two to three green areas represent the low frequency field of A1 (A1 LF), the secondary auditory cortex (A2), and the anterior auditory field (AAF). The red area represents the high frequency field of A1 (A1 HF).…”

Section: Resultsmentioning

confidence: 99%

Homeostatic plasticity and synaptic scaling in the adult mouse auditory cortex

Teichert¹,

Liebmann

Hübner

et al. 2017

Sci Rep

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It has been demonstrated that sensory deprivation results in homeostatic adjustments recovering neuronal activity of the deprived cortex. For example, deprived vision multiplicatively scales up mEPSC amplitudes in the primary visual cortex, commonly referred to as synaptic scaling. However, whether synaptic scaling also occurs in auditory cortex after auditory deprivation remains elusive. Using periodic intrinsic optical imaging in adult mice, we show that conductive hearing loss (CHL), initially led to a reduction of primary auditory cortex (A1) responsiveness to sounds. However, this was followed by a complete recovery of A1 activity evoked sounds above the threshold for bone conduction, 3 days after CHL. Over the same time course patch-clamp experiments in slices revealed that mEPSC amplitudes in A1 layers 2/3 pyramids scaled up multiplicatively in CHL mice. No recovery of sensory evoked A1 activation was evident in TNFα KO animals, which lack synaptic scaling. Additionally, we could show that the suppressive effect of sounds on visually evoked visual cortex activity completely recovered along with TNFα dependent A1 homeostasis in WT animals. This is the first demonstration of homeostatic multiplicative synaptic scaling in the adult A1. These findings suggest that mild hearing loss massively affects auditory processing in adult A1.Homeostatic plasticity is essential in maintaining a stable firing rate in neurons to compensate for prolonged perturbations of neuronal activity 1,2 . For example, a prolonged reduction of neuronal activity of cultured neocortical neurons generated compensatory changes returning firing levels back to control values 1 . Such homeostatic regulations are typically accompanied by an additional insertion of 2-amino-3-(3-hydroxy-5-methyl-isoxasol-4-yl) propanoic acid receptors (AMPARs) into all synapses of a neuron, leading to multiplicatively scaled up mEPSC (miniature excitatory postsynaptic currents) amplitudes or, "synaptic scaling" 2,3 . Specifically, this mechanism is described to globally increase (or decrease) the strength of each synapse of a neuron by the same factor in a multiplicative manner which allows the conservation of information stored in the synaptic weights 4 . Previous studies have demonstrated that synaptic scaling also takes place in vivo, e.g. after reduction of sensory cortex activity due to sensory deprivation. For example, visual deprivation by intraocular TTX injections, dark exposure, binocular enucleation or retinal lesions scales up AMPAR mediated mEPSC amplitudes in layers 2/3 pyramids of the primary visual cortex (V1) in juvenile and adult mice [5][6][7][8][9] . Furthermore, it was shown that prolonged visual deprivation increases visually evoked V1 responses during the visual critical period 10 . However, evidence for homeostatic synaptic scaling in primary auditory cortex and its effects on cortical responsiveness following auditory deprivation is rare.Previously, two models for induction of an auditory deprivation have been used, sensorineural hearing...

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Delineation of a frequency-organized region isolated from the mouse primary auditory cortex

Cited by 66 publications

References 84 publications

Reciprocal connectivity between secondary auditory cortical field and amygdala in mice

Reciprocal connectivity between secondary auditory cortical field and amygdala in mice

Multiscale mapping of frequency sweep rate in mouse auditory cortex

Homeostatic plasticity and synaptic scaling in the adult mouse auditory cortex

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