Delineation of Cell Cycle State and Correlation to Adhesion Molecule Expression of Human CD34⁺Cells from Steady‐State Bone Marrow and Peripheral Blood Mobilized Following G‐CSF‐Supported Chemotherapy

Abstract

ABSTRACT

Mobilization of CD34+ haematopoietic stem cells is associated with a functional inactivation of the integrin very late antigen 4

“…A similar finding was made with respect to the VLA-4 expression level as CD34 1 cells in S 1 G2/M phase expressed more VLA-4 than cells in G0/G1 phase (Fruehauf et al, 1998;Yamaguchi et al, 1998). Moreover, VLA-4 function was not only associated with the cell cycle but also with the maturation state of CD34 1 cells.…”

“…To compare expression levels of a4 and VCAM±Ig binding properties of samples from different time points or different donors, the mean fluorescence intensity was normalized by dividing by the mean fluorescence intensity of the respective negative control, resulting in the relative mean fluorescence intensity. Cell cycle analysis was performed as described previously using the DNA staining dye 7-aminoactinomycin D (7-AAD; Molecular Probes, Leiden, The Netherlands) (Fruehauf et al, 1998).…”

Adhesion to fibronectin stimulates proliferation of wild-type and bcr/abl -transfected murine hematopoietic cells

“…They propose a two-step model with an initial activation of ␣ 4 ␤ 1 -and ␣ 5 ␤ 1 -integrins by cytokines followed by a secondary signal resulting from integrin-fibronectin binding that may cooperate with those generated by cytokine receptors. We have reported previously that, in contrast to human marrow-adherent CD34 ϩ cells, the majority of peripheral blood CD34 ϩ cells mobilized after granulocyte-colonystimulating factor-supported chemotherapy is arrested in the late G 1 phase of the cell cycle and fibronectin-mediated activation via ␣ 4 and ␣ 5 integrins seems to be a major pathway that enables G 1 ͞S transition (48). These findings are in agreement with the data presented here, because adhesion to fibronectin stimulated cell cycle progression of both parental and p210bcr͞abl-transfected 32D cells at the G 1 ͞S boundary.…”

Proliferating status of peripheral blood progenitor cells from patients with BCR/ABL-positive chronic myelogenous leukemia

“…For intracellular antigen staining and DNA analysis with 7-aminoactinomycin D (7-AAD, MoBiTec, Gö ttingen, Germany), a fixation and permeabilization protocol reported previously was followed. 19 In brief, following surface antigen staining, CD34…”

“…18,19 Furthermore, HPC from mice treated with cyclophosphamide and G-CSF proliferated only in the BM and spleen whereas HPC released into PB tended to be arrested in the G 0 /G 1 phase over an observation period of 8 days. 21 Interestingly, To and coworkers 22 have described that this cell cycle arrest of normal human PBPC is not ascribable either to inhibitory elements in the blood or to reduced responsiveness to growth factors.…”