Delivery of genome editing tools by bacterial extracellular vesicles

Liu, Y.; Smid, Eddy J.; Abee, Tjakko; Notebaart, Richard A.

doi:10.1111/1751-7915.13356

Cited by 13 publications

(10 citation statements)

References 21 publications

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“…Available data however suggests a significant impact on the biogeochemical cycles. EVs are potentially involved in cell communication, competition and survival of bacteria (Liu et al, 2019). Interactions between ultramicro-prokaryotes and other micro-organisms communities may shape natural microbiome function (Castelle et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Discovery of High Abundances of Aster-Like Nanoparticles in Pelagic Environments: Characterization and Dynamics

et al. 2019

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This study reports the discovery of Aster-Like Nanoparticles (ALNs) in pelagic environments. ALNs are pleomorphic, with three dominant morphotypes which do not fit into any previously defined environmental entities [i.e., ultramicro-prokaryotes, controversed nanobes, and non-living particles (biomimetic mineralo-organic particles, natural nanoparticles or viruses)] of similar size. Elemental composition and selected-area electron diffraction patterns suggested that the organic nature of ALNs may prevail over the possibility of crystal structures. Likewise, recorded changes in ALN numbers in the absence of cells are at odds with an affiliation to until now described viral particles. ALN abundances showed marked seasonal dynamics in the lakewater, with maximal values (up to 9.0 ± 0.5 × 107 particles·mL−1) reaching eight times those obtained for prokaryotes, and representing up to about 40% of the abundances of virus-like particles. We conclude that (i) aquatic ecosystems are reservoirs of novel, abundant, and dynamic aster-like nanoparticles, (ii) not all virus-like particles observed in aquatic systems are necessarily viruses, and (iii) there may be several types of other ultra-small particles in natural waters that are currently unknown but potentially ecologically important.

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Section: Introductionmentioning

confidence: 99%

Discovery of High Abundances of Aster-Like Nanoparticles in Pelagic Environments: Characterization and Dynamics

et al. 2019

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“…Extracellular vesicles (EV) in Gram-negative bacteria have been studied for their pathogenicity, host-pathogen interaction and potential targets in vaccine development 31 . Very recently, studies on EV were performed in probiotic strains and revealed potential delivery of bacteriocins and other beneficial properties through the EV 32,33 . Advancing research on EV of probiotics is highly recommended as it will provide further understanding on molecular mechanisms of probiotic bactericidal properties against pathogens and immune modulation.…”

Section: Mechanisms Of Probiotics In Interfering With Pathogens and Imentioning

confidence: 99%

The relevance of probiotics in Caesarean-born neonates

et al. 2020

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There is growing interest in the use of probiotics in neonates. In particular, Lactobacillus rhamnosus, L. acidophilus, Bifidobacterium breve and B. longum have been well studied. Caesarean-section (CS)-born infants often lack Lactobacillus spp. and Bifidobacterium spp., which showed increasing evidence in establishing the neonatal immune system. Furthermore, CS increases the difficulties for mothers in initiating and sustaining breastfeeding. Increasing evidence shows CS-born infants are more susceptible to allergy, infections and chronic inflammatory diseases later in life. The number of CS births has increased continuously, now accounting for 35% of all deliveries Australia wide. In this context, probiotics may have a role in establishing a healthy neonatal gut microbiome.

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“…Zukünftig sollen Membranvesikel auch für die Genom‐Editierung, also die beliebige Veränderung, das Entfernen oder Einfügen von Genen in Chromosomen, eingesetzt werden . Die zur Zeit effizienteste Methode der Genom‐Editierung beruht auf einem System, das viele Bakterienspezies entwickelt haben, um Fremd‐DNA, die entweder durch Infektion mit Phagen oder über horizontalen Gentransfer in diese eindringt, zu erkennen und abzubauen (Abbildung ).…”

Section: Membranvesikel Und Gen‐editierungunclassified

Bakterielle Membranvesikel

Roth¹,

Schumann

2019

Biologie in unserer Zeit

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Zusammenfassung Zellen aller drei Domänen des Lebens (Eukaryoten, Bakterien und Archaeen) produzieren und entlassen Membranvesikel, die von einer Lipid‐Doppelschicht umgeben sind. Diese sind meist sphärisch mit einem Durchmesser von 20–500 nm und können in ihrem Innern, dem Lumen, verschiedene Moleküle transportieren, die in ihrer Gesamtheit als Cargo bezeichnet werden. So enthalten sie in der Regel verschiedene Proteine, Polysaccharide und Metabolite, aber auch zusätzlich Nukleinsäuren (DNA, RNA) und manchmal fehlgefaltete Proteine. Sie spielen eine wichtige Rolle beim horizontalen Gentransfer und bei der Pathogenität. Außerdem können sie Phagenrezeptoren in Phagen‐resistente Zellen und nahe verwandte Spezies übertragen. Weltweit erforschen zahlreiche Firmen ihre Anwendung als Überträger von Impfstoffen beim Menschen. Zudem wird untersucht, inwieweit Membranvesikel bei der Genom‐Editierung eingesetzt werden können.

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Delivery of genome editing tools by bacterial extracellular vesicles

Cited by 13 publications

References 21 publications

Discovery of High Abundances of Aster-Like Nanoparticles in Pelagic Environments: Characterization and Dynamics

Discovery of High Abundances of Aster-Like Nanoparticles in Pelagic Environments: Characterization and Dynamics

The relevance of probiotics in Caesarean-born neonates

Bakterielle Membranvesikel

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