2006
DOI: 10.4161/cc.5.18.3192
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Delivery of RNA Interference

Abstract: Over the last few years, RNA Interference (RNAi), a naturally occurring mechanism of gene regulation conserved in plant and mammalian cells, has opened numerous novel opportunities for basic research across the field of biology. While RNAi has helped accelerate discovery and understanding of gene functions, it also has great potential as a therapeutic and potentially prophylactic modality. Challenging diseases failing conventional therapeutics could become treatable by specific silencing of key pathogenic gene… Show more

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Cited by 168 publications
(99 citation statements)
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References 57 publications
(59 reference statements)
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“…3-5 Initially, long dsRNA is recognized and cleaved by the RNase III enzyme Dicer into 21-to 24-nucleotide duplexes of siRNA. [3][4][5] The antisense siRNA strand is incorporated into the RNA-induced silencing complex (RISC) which then targets complementary mRNA sequences in the cytoplasm. mRNA is then cleaved by RNase at sites not bound by siRNA thus preventing protein translation.…”
Section: Introductionmentioning
confidence: 99%
“…3-5 Initially, long dsRNA is recognized and cleaved by the RNase III enzyme Dicer into 21-to 24-nucleotide duplexes of siRNA. [3][4][5] The antisense siRNA strand is incorporated into the RNA-induced silencing complex (RISC) which then targets complementary mRNA sequences in the cytoplasm. mRNA is then cleaved by RNase at sites not bound by siRNA thus preventing protein translation.…”
Section: Introductionmentioning
confidence: 99%
“…2,7 Alternatively, viral vectors, such as adenoviral vectors, AAV vectors and lenti-or retroviral vectors are being used encoding the genetic information for the RNAi of choice as shRNA. 8 Murine leukaemia virus (MLV) is a simple, well-characterised g-retrovirus, which has been used in numerous studies of cancer therapy and gene transfer. Due to its intrinsic inability to infect quiescent cells, MLV is especially suitable for cancer research as it preferentially infects tumour cells.…”
Section: Introductionmentioning
confidence: 99%
“…Although there has been progress with direct local application of therapeutic siRNA for a limited number of indications, the application of RNAi-based therapies in vivo has been hampered due to difficulties with delivery, the cost of manufacturing large quantities of siRNA and the activation of non-specific interferon responses (Shankar et al, 2005;Li et al, 2006). Of these, delivery has proven to be the main obstacle.…”
Section: Introductionmentioning
confidence: 99%