Delivery of short hairpin RNAs by transkingdom RNA interference modulates the classical ABCB1-mediated multidrug-resistant phenotype of cancer cells

RNA-mediated regulation in bacteria: from natural to artificial systems

“…One can guess that such engineered bacteria may be used as live vaccines, to deliver gene vectors, or cytotoxic drugs. Indeed, recent strategies have used non-pathogenic bacteria to deliver short hairpin RNA from DNA constructs to specifically inhibit gene expression in cancer derived cells [86].…”

Knockdown of MDR1 Increases the Sensitivity to Adriamycin in Drug Resistant Gastric Cancer Cells

“…This poor prognosis is mainly due to MDR in gastric cancer cells. Many strategies have been developed to overcome MDR in cancer, including modification of chemotherapy regimens (Dong and Mumper, 2010), inactivation o MDR-associated genes (Kruhn et al, 2009;Stege et al, 2010), use of monoclonal antibodies for MDR1 (Goda et al, 2007), new inhibitors of ABC transporters (Dong and Mumper, 2010) and so on. However, there are numerous challenges for these strategies.…”

Analysis of Important Gene Ontology Terms and Biological Pathways Related to Pancreatic Cancer

“…As for the protein level, western blot turns out to be the most commonly used biochemical method to identify the expression and activation status of a known protein in certain in vivo or in vitro environment. Further, relying on in vitro gene expression (RNA) interference technologies, the characteristic alteration of the expression and function of a series of proteins that have been identified on such two levels as we have mentioned above can be validated and such group of proteins can be further concluded into various biological processes and pathways [9, 10]. Based on the experimental technologies we have mentioned above, various principal regulatory pathways have been identified and confirmed to contribute to the initiation and progression of pancreatic cancer.…”