Delivery systems of current biologicals for the treatment of chronic cutaneous wounds and severe burns

Xue, Meilang; Zhao, Ruilong; Lin, Hui; Jackson, Christopher J.

doi:10.1016/j.addr.2018.03.002

Cited by 85 publications

(71 citation statements)

References 388 publications

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“…Hydrogel wound dressings can provide a moist local environment for the wound surface, promoting skin renewal and cell proliferation making them increasingly one of the best treatment options to promote wound healing. 40,41 However, excessive activation of inammatory signals can cause chronic inammation and thus impair cutaneous wound healing. 42 Therefore, promote cell proliferation and block inammation signal activation using wound dressing materials may offer powerful new treatment modalities for wound healing.…”

Section: Resultsmentioning

confidence: 99%

Green preparation of anti-inflammation an injectable 3D porous hydrogel for speeding up deep second-degree scald wound healing

Che

et al. 2020

RSC Adv.

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Section: Resultsmentioning

confidence: 99%

Green preparation of anti-inflammation an injectable 3D porous hydrogel for speeding up deep second-degree scald wound healing

Che

et al. 2020

RSC Adv.

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“…TGF-β and IL-10 were chosen for their ability to regulate immune response and suppress inflammation. In contrast, VEGF and FGF were selected for their ability to stimulate neovascularization and tissue regeneration (Yu et al, 2017;Niu et al, 2018;Xue et al, 2018). All incorporated GF/C exhibit a sustained release profile with gradual increase in the FIGURE 8 | Wound healing progression.…”

Section: Discussionmentioning

confidence: 99%

Sequential Delivery of Cryogel Released Growth Factors and Cytokines Accelerates Wound Healing and Improves Tissue Regeneration

Jimi

Jaguparov

Nurkesh

et al. 2020

Front. Bioeng. Biotechnol.

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Growth factors and cytokines that are secreted by cells play a crucial role in the complex physiological reaction to tissue injury. The ability to spatially and temporally control their actions to maximize regenerative benefits and minimize side effects will help accelerate wound healing and improve tissue regeneration. In this study, the sequential targeted delivery of growth factor/cytokine combinations with regulatory functions on inflammation and tissue regeneration was examined using an internal splint wound healing model. Four examined growth factors and cytokines were effectively incorporated into a novel chitosan-based cryogel, which offered a controlled and sustained release of all factors while maintaining their biological activities. The cryogels incorporated with inflammation modulatory factors (IL-10 and TGF-β) and with wound healing factors (VEGF and FGF) were placed on the wound surface on day 0 and day 3, respectively, after wound initiation. Although wound area gradually decreased in all groups over time, the area in the cryogel group with growth factor/cytokine combinations was significantly reduced starting on day 7 and reached about 10% on day 10, as compared to 60-65% in the control groups. Sequential delivery of inflammation modulatory and wound healing factors enhanced granulation tissue formation, as well as functional neovascularization, leading to regenerative epithelialization. Collectively, the chitosan-based cryogel can serve as a controlled release system for sequential delivery of several growth factors and cytokines to accelerate tissue repair and regeneration.

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“…Scaffold-based stem cell delivery has become increasingly popular. This method offers several advantages over other techniques, both in animal models of wound healing and in clinical settings, including complete wound coverage, protection of transplanted cells, preserved cell expression of stemness-related genes, and significantly accelerated wound healing (Xue et al, 2018;Mulholland, 2020).…”

Section: Introductionmentioning

confidence: 99%

Electrospun PCL/PLA Scaffolds Are More Suitable Carriers of Placental Mesenchymal Stromal Cells Than Collagen/Elastin Scaffolds and Prevent Wound Contraction in a Mouse Model of Wound Healing

Vonbrunn

Mueller

Pichlsberger

et al. 2020

Front. Bioeng. Biotechnol.

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Mesenchymal stem/stromal cells (MSCs) exert beneficial effects during wound healing, and cell-seeded scaffolds are a promising method of application. Here, we compared the suitability of a clinically used collagen/elastin scaffold (Matriderm) with an electrospun Poly(ε-caprolactone)/poly(l-lactide) (PCL/PLA) scaffold as carriers for human amnion-derived MSCs (hAMSCs). We created an epidermal-like PCL/PLA scaffold and evaluated its microstructural, mechanical, and functional properties. Sequential spinning of different PCL/PLA concentrations resulted in a wide-meshed layer designed for cell-seeding and a dense-meshed layer for apical protection. The Matriderm and PCL/PLA scaffolds then were seeded with hAMSCs, with or without Matrigel coating. The quantity and quality of the adherent cells were evaluated in vitro. The results showed that hAMSCs adhered to and infiltrated both scaffold types but on day 3, more cells were observed on PCL/PLA than on Matriderm. Apoptosis and proliferation rates were similar for all carriers except the coated Matriderm, where apoptotic cells were significantly enhanced. On day 8, the number of cells decreased on all carrier types except the coated Matriderm, which had consistently low cell numbers. Uncoated Matriderm had the highest percentage of proliferative cells and lowest apoptosis rate of all carrier types. Each carrier also was topically applied to skin wound sites in a mouse model and analyzed in vivo over 14 days via optical imaging and histological methods, which showed detectable hAMSCs on all carrier types on day 8. On day 14, all wounds exhibited newly formed epidermis, and all carriers were well-integrated into the underlying dermis and showing signs of degradation. However, only wounds treated with uncoated PCL/PLA maintained a round appearance with minimal contraction. Overall, the results support a 3-day in vitro culture of scaffolds with hAMSCs before wound application. The PCL/PLA scaffold showed higher cell adherence than Matriderm, and the effect of the Matrigel coating was negligible, as all carrier types maintained sufficient numbers of transplanted cells in the wound area. The anti-contractive effects of the PCL/PLA scaffold offer potential new therapeutic approaches to wound care.

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Delivery systems of current biologicals for the treatment of chronic cutaneous wounds and severe burns

Cited by 85 publications

References 388 publications

Green preparation of anti-inflammation an injectable 3D porous hydrogel for speeding up deep second-degree scald wound healing

Green preparation of anti-inflammation an injectable 3D porous hydrogel for speeding up deep second-degree scald wound healing

Sequential Delivery of Cryogel Released Growth Factors and Cytokines Accelerates Wound Healing and Improves Tissue Regeneration

Electrospun PCL/PLA Scaffolds Are More Suitable Carriers of Placental Mesenchymal Stromal Cells Than Collagen/Elastin Scaffolds and Prevent Wound Contraction in a Mouse Model of Wound Healing

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