Delphinidin prevents high glucose-induced cell proliferation and collagen synthesis by inhibition of NOX-1 and mitochondrial superoxide in mesangial cells

Song, Suk-Won; Jo, Hye Jun; Kim, Yong-Woon; Cho, Young-Je; Kim, Jae-Ryong; Park, Soyoung

doi:10.1016/j.jphs.2016.03.005

Cited by 20 publications

(7 citation statements)

References 37 publications

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“…HG was recognized not only as the main characteristic of DN but also as a risk factor for the occurrence and development of DN [18]. Research con rmed HG can promote proliferation of HMCs [19][20][21]. Our study provides evidence that TRL can inhibit HG-induced HMC overproliferation in a concentration-dependent manner.…”

Section: Discussionsupporting

confidence: 60%

Trigonelline regulates Wnt/β-catenin signaling in glomerular mesangial cells under high glucose conditions

Chen¹,

Shi²,

Chen³

et al. 2021

Preprint

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Background: Trigonelline have hypoglycemic effects. In previous animal experiments, we observed that trigonelline (TRL) treat-ment attenuated metabolic abnormalities associated with hyperglycemic conditions in the experimental DN model. In streptozotocin (STZ)-induced rats, TRL treatment reduced albuminuria, lowered blood sugar, improved renal function and alleviated the pathological alterations within the glomerulus. Methods: We stimulated human mesangial cells (HMC) with high glucose (30 mmol / L) medium. HMCs were transfected with β-catenin plasmid or siRNA to investigate the effect of trigonelline on high glucose-induced excessive proliferation and apoptosis of HMCs, and to understand its mechanism of action. Cell viability was measured by MTT assay. Flow cytometry was used to detect the cell cycle. Cell apoptosis was evaluated by flow cytometry and terminal dUTP transferase nick end labeling (TUNEL) assay. Protein and mRNA expression of β-catenin, Wnt5a, TCF4, Cyclin D1, and CDK4 were detected by western blotting and RT-PCR, respectively. Results: Trigonelline inhibited cell proliferation by blocking cell-cycle progression at the G1 phase and decreased apoptosis via the Wnt/β-catenin pathway. Elevated glucose levels enhanced the expression of β-catenin, an important modulator of diabetic nephropathy, while trigonelline restored up-regulation. Conclusions: High glucose and high expression of β-catenin could lead to cell injury; however, this effect was mitigated by trigonelline via managing the canonical Wnt/β-catenin signaling pathway.

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Section: Discussionsupporting

confidence: 60%

Trigonelline regulates Wnt/β-catenin signaling in glomerular mesangial cells under high glucose conditions

Chen¹,

Shi²,

Chen³

et al. 2021

Preprint

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“…The delphinidin (delph) aglycone has also been studied for its antioxidant properties in other tissue models of diabetes [98]. In a cellular model of diabetic nephropathy, 24 h incubation of mesangial (glomerular) cells with 25 mM glucose induces cellular proliferation and excess extracellular matrix, which are triggered by mitochondrial stress stemming from NADPH oxidase 1 (NOX1) activation and ROS generation.…”

Section: Anthocyanins Block Reactive Oxygen Species and Protect Mitocmentioning

confidence: 99%

Anthocyanin Bioactivity in Obesity and Diabetes: The Essential Role of Glucose Transporters in the Gut and Periphery

Solverson

2020

Cells

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Obesity and type-2 diabetes trends continue to worsen in the United States. Dietary anthocyanins (typically provided by berries and other fruits) are reported to have protective effects against both conditions using a variety of experimental research models including animal and human feeding studies. This review highlights studies that explore the biochemical pathways in both tissue and rodent models which could explain clinical improvements noted with anthocyanin consumption. First, the primary mode of intestinal absorption of anthocyanins is through both sGLT1 and GLUT2 glucose transporters. Stronger binding affinities may allow anthocyanins to be more inhibitive to glucose absorption compared to the reverse, where GLUT2 expression may also be affected. Genetic or chemical inhibition of sGLT1 or GLUT2 demonstrate their essential function in anthocyanin absorption across the enterocyte, where the former interacts with a greater variety of anthocyanins but the latter is the major transporter for specific anthocyanin-glycosides. Once absorbed, anthocyanins positively modulate GLUT4 density and function in both skeletal muscle and adipose tissues via the upregulation of AMPK and restoration of insulin sensitivity. Antioxidant properties and phosphodiesterase inhibition by anthocyanins promote both mitochondrial function and density which could be novel targets for dietary management of obesity and its complications.

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“…The delphinidins evidenced, in previous studies, several bioactive properties, being the anthocyanin compound with the greatest beneficial effect in preventing cancer, being related to the death of apoptotic or autophagic cells in various types of cancer. In addition, it has antioxidant and anti-inflammatory action [58,59].…”

Section: Profile In Non-anthocyanin and Anthocyanin Phenolic Compoundsmentioning

confidence: 99%

Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies

et al. 2020

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Consumers are very concerned with following a healthy diet, along with some precautions that may influence environmental impact. Solanum melongena L. is one of the most consumed vegetables due to its excellent nutritional value and antioxidant action. Associated with its high consumption, considerable amounts of agro-food wastes are produced. This work targets the valorization of this matrix, through the use of its bio-residues to study the obtention of coloring pigments, applying innovative technologies. Its nutritional value, chemical composition, and bioactive potential were evaluated, and the ultrasound-assisted extraction to obtain coloring pigments of high industrial interest was optimized. Considering the results, low contents of fat and carbohydrates and energy value were evident, as well as the presence of compounds of interest (free sugars, organic acids, unsaturated fatty acids, and phenolic acids). In addition, the antioxidant and antimicrobial potential was detected. Response surface methodology was performed to optimize the extraction of natural pigments, showing a concentration of 11.9 mg/g of anthocyanins/g of extract, applying optimal conditions of time, solvent, and solid/liquid ratio of 0.5 min, 68.2% (v/v) and 5 g/L, respectively. S. melongena proved to be a good source of bioactive compounds and natural pigments, which can generate great interest in the food industry.

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Delphinidin prevents high glucose-induced cell proliferation and collagen synthesis by inhibition of NOX-1 and mitochondrial superoxide in mesangial cells

Cited by 20 publications

References 37 publications

Trigonelline regulates Wnt/β-catenin signaling in glomerular mesangial cells under high glucose conditions

Trigonelline regulates Wnt/β-catenin signaling in glomerular mesangial cells under high glucose conditions

Anthocyanin Bioactivity in Obesity and Diabetes: The Essential Role of Glucose Transporters in the Gut and Periphery

Eggplant Fruit (Solanum melongena L.) and Bio-Residues as a Source of Nutrients, Bioactive Compounds, and Food Colorants, Using Innovative Food Technologies

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