Delta-Aminolevulnic Acid Dehydrase in Greening Bean Leaves

Steer, B. T.; Gibbs, Martin

doi:10.1104/pp.44.5.781

Cited by 25 publications

(5 citation statements)

References 10 publications

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“…Among the best documented of these are 5-aminolaevulinate dehydratase (Steer & Gibbs, 1969), porphobilinogen deaminase (Schneider, 1971), and chlorophyllase (Stobart & Thomas, 1968). The decrease in the specific activity of the enzyme protochlorophyllide reductase observed during illumination of etiolated plants in the present work (Fig.…”

Section: Discussionsupporting

confidence: 49%

Light modulation of the activity of protochlorophyllide reductase

Mapleston¹,

Griffiths²

1980

Biochemical Journal

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Section: Discussionsupporting

confidence: 49%

Light modulation of the activity of protochlorophyllide reductase

Mapleston¹,

Griffiths²

1980

Biochemical Journal

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“…PChlide formation irradiation, h formation during ALA-treatment 0.15 0.32 0.25 0.30 is in accordance with an increased activity of ALA-dehydrase during chlorophyll formation (Steer 1969).…”

Section: Time Of Chisupporting

confidence: 72%

The Pool Size of Protochlorophyllide during Different Stages of Greening of Dark Grown Wheat Leaves

Sundqvist

1974

Physiologia Plantarum

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The pool size of protochlorophyllide in wheat leaves irradiated for 5 minutes to 6 hours was studied. Protochlorophyllide then accumulated in the dark, but the pool size of regenerated protochlorophyllide was considerably smaller in leaves irradiated for six hours than in leaves irradiated for 5 minutes. The decrease in pool size of regenerated protochlorophyllide was found to take place at the time when the chlorophyll formation had accelerated and reached the linear phase. The protochlorophyllide accumulated is the form with absorption maximum at 650 nm, which is phototransformed to chlorophyllide with maximum absorption at 684 nm. This species goes through the Shibata shift when formed even after 6 hours of irradiation. If leaves, irradiated for 1 or 6 hours, were fed with δ‐amino‐levulinic acid the protochlorophyllide synthesis was only 1.2 times faster in the leaves irradiated for 6 hours than in those irradiated for 1 hour. In the case of leaves fed with δ‐amino‐levulinic acid the absorption maximum of protochlorophyllide is at 636 nm and the absorption maximum of the chlorophyllide formed is at 672 nm.

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“…Steer and Gibbs (21) were able to demonstrate changes in ALA dehydrase activity in greening bean leaves, but these changes were not sufficiently large to indicate a regulatory function for this enzyme. Our finding that ALA dehydrase activity is maintained at a constant level as etiolated cucumber cotyledons are allowed to become green, supports the hypothesis that this enzyme is not involved in the regulation of the Chl pathway.…”

Section: Discussionmentioning

confidence: 99%

The Biosynthesis of δ-Aminolevulinic Acid in Higher Plants

Beale¹,

Castelfranco²

1974

Plant Physiol.

119

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8-Aminolevulinic acid dehydrase activity in cucumber (Cucumis sativus L. var. Alpha green) cotyledons did not change as the tissue was allowed to green for 24 hours. 5-Aminolevulinic acid accumulated in greening cucumber cotyledons, and barley (Hordeum sativum L. var. Numar) and bean (Phaseolus vulgaris L. var. Red Kidney) leaves incubated in the presence of levulinic acid, a specific competitive inhibitor of 5-aminolevulinic acid dehydrase. The rate of 5-aminolevulinic acid accumulation in levulinic acid-treated cucumber cotyledons paralleled the rate of chlorophyll accumulation in the controls, and the quantitv of 5-aminolevulinic acid accumulated compensated for the decrease in chlorophyll accumulation. When levulinic acid-treated cucumber cotyledons were returned to darkness, 5-aminolevulinic acid accumulation ceased.8-Aminolevulinic acid accumulation showed an absolute requirement for oxygen and was inhibited drastically by cyanide and azide, and to a lesser extent by arsenite and malonate.2,4-Dinitrophenol, 3-(3,4.dichlorophenyl)-1, 1-dimethyl urea, sodium fluoroacetate, and hydroxylamine hydrochloride showed no effect under the conditions tested. Freezing and thawing of the tissue completely prevented the accumulation of 5-aminolevulinic acid.The findings of this investigation are consistent with the hypothesis that 5-aminolevulinic acid is a chlorophyll precursor in higher plants, and that chlorophyll biosynthesis is regulated at the level of the formation of 5-aminolevulinic acid.

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Delta-Aminolevulnic Acid Dehydrase in Greening Bean Leaves

Cited by 25 publications

References 10 publications

Light modulation of the activity of protochlorophyllide reductase

Light modulation of the activity of protochlorophyllide reductase

The Pool Size of Protochlorophyllide during Different Stages of Greening of Dark Grown Wheat Leaves

The Biosynthesis of δ-Aminolevulinic Acid in Higher Plants

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