Deltamethrin-induced testicular apoptosis in rats: the protective effect of nitric oxide synthase inhibitor

El-Gohary, Mona; Awara, Wageh M.; Nassar, Samia; Hawas, Samia

doi:10.1016/s0300-483x(98)00114-0

Cited by 108 publications

(52 citation statements)

References 30 publications

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“…Clark et al (2007) demonstrated increased hepatic upregulation of p21 and Bax in mice after 28 days exposure to MC-LR at sublethal levels. There was also a study that suggests that the testicular apoptosis might be closely related to nitric oxide (NO) in rats (El-Gohary et al, 1999). Moreover, we previously confirmed that MCs induce the expression of Fas/FasL system related genes at both mRNA and protein level in rat testis, indicating the likely participation of the Fas/FasL system in MCs-induced germ cell apoptosis (Xiong et al, 2009).…”

Section: Discussionsupporting

confidence: 54%

Involment of p53, Bax, and Bcl‐2 pathway in microcystins‐induced apoptosis in rat testis

Xie

et al. 2011

Environmental Toxicology

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It has been reported that microcystins (MCs) could accumulate in the gonads of mammals and MCs exposure exerts obvious toxic effects on male reproductive system of mammals. We have comfirmed that MCs could accumulate and induce apoptosis in rat testis. The p53, Bax, and Bcl-2 protein play important roles in mitochondria-dependent apoptotic pathway, and this study aimed to investigate whether the p53, Bax, and Bcl-2 pathway is involved in microcystins-induced apoptosis in rat testis and discussed the possible mechanisms. Our results show that MCs led to persistent increase of transcriptional and protein level of P53 and Bax expression but led to decrease of Bcl-2 expression, resulting in an increased ratio of Bax to Bcl-2, which might contribute to apoptotic cell death of rat testis following MCs treatment. The increased ratio of expression of Bax to that of Bcl-2 induced by MCs suggests their important role in MCs-induced apoptosis in rat testis tissue.

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Section: Discussionsupporting

confidence: 54%

Involment of p53, Bax, and Bcl‐2 pathway in microcystins‐induced apoptosis in rat testis

Xie

et al. 2011

Environmental Toxicology

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“…Also DM intoxication caused significant increase in NO concentration [ Table-2]. This result was agreed with [30][31][32]. Possible reasons for reduction of TAC and increases of NO might be the utilization antioxidant enzymes to challenge the prevailing oxidative stress under the influence of free radicals generated from DM and/or inhibition of enzyme synthesis by DM [28,[33][34][35].…”

Section: Discussionsupporting

confidence: 60%

“…The possible mechanism of DM genotoxicity is either to its reaction with DNA or by the generation of ROS which caused DNA damage [54][55][56]. The higher level of NO produced through DM intoxication inhibits cellular respiration and triggers apoptosis causing DNA damage [30]. Such oxidative DNA damage, if not repaired before replication, eventually result in mutations and initiate carcinogenesis.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Zinc as Antioxidant Against the Genotoxic Potential of Deltamethrin

Ibrahim¹,

Khalaf²,

Galal³

et al. 2014

Int J Mol Biol

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“…NO has been retained to play in the testis the following functions: a) inhibition of steroidogenesis, b) stimulation of germ cell metabolism/apoptosis, c) relaxation of vascular myocytes, d) regulation of peristalsis and permeability of the seminiferous tubule wall (Adams et al 1994;Welch et al 1995;Rosselli et al 1995;Del Punta et al 1996;Middendorff et al 1997;Davidoff et al 1997;Lissbrandt et al 1997;Pomerantz and Pitelka 1998;El Gohary et al 1999). The role played by NO in the steroidogenesis inhibition has primary importance in the species reproduction.…”

Section: Discussionmentioning

confidence: 99%

“…In the testis NO has been thought to influence testosterone synthesis, blood circulation, seminiferous tubule contraction and germ cell metabolism/apoptosis (Adams et al 1994;Davidoff et al 1995;Rosselli et al 1995;El Gohary et al 1999). The ways by which the oxide plays these roles are incompletely understood as yet.…”

mentioning

confidence: 99%

Isoforms of Nitric Oxide Synthase in the Pig Testis

Ambrosino¹,

Russo²,

Lamanna³

et al. 2003

Acta Vet. Brno

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Nitric oxide is a biologically active molecule involved in the regulation of main functions of the body and is produced in different cellular types by the action of molecular isoforms of the enzyme nitric oxide synthase (NOS). The presence of the isoforms defined as neuronal, testicular, endothelial and inducible has been studied in the testis of adult pigs by means of immunohistochemical techniques. Western blot analysis and a biochemical quantitative method were also performed to confirm the morphological data and to assess nitric oxide tissue activity.The NOS isoforms were contained in interstitial (Leydig), sustentacular (Sertoli), peritubular, endothelial and macrophage cells, as well as in spermatogonia and spermatids. Positive Leydig cells were numerous, isolated or grouped in clusters and differed in staining intensity. Testicular extracts were found to contain four types of proteins whose molecular weight was very similar to those of the NOS isoforms. The biochemical measurement of the NO 2 tissue content gave the result of 155.25 ± 21.9 nmol/mg.On the basis of a detailed bibliographic review, the findings here obtained led us to hypothesize that a) the sources of nitric oxide in the pig testis are numerous, and b) the oxide could be involved in the regulation of the following testicular functions: steroidogenesis, vasodilatation, peristalsis/permeability of seminiferous tubules and development/apoptosis of germ cells.

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Deltamethrin-induced testicular apoptosis in rats: the protective effect of nitric oxide synthase inhibitor

Cited by 108 publications

References 30 publications

Involment of p53, Bax, and Bcl‐2 pathway in microcystins‐induced apoptosis in rat testis

Involment of p53, Bax, and Bcl‐2 pathway in microcystins‐induced apoptosis in rat testis

Protective Effects of Zinc as Antioxidant Against the Genotoxic Potential of Deltamethrin

Isoforms of Nitric Oxide Synthase in the Pig Testis

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