Deltonin induced both apoptosis and autophagy in head and neck squamous carcinoma FaDu cell

Abstract

For decades, despite the advancement of medical science, the prognosis of head and neck squamous cell carcinoma (HNSCC), has not improved. Deltonin is one of the major active components of Dioscorea Zingiberensis Wright that has been used for anthrax, rheumatic heart disease, rheumatoid arthritis etc. By employing HNSCC FaDu cell and normal human epidermal keratinocyte, we investigate deltonin efficacy and associated mechanism in both cell culture and nude mice xenografts. Deltonin treatment selectively prevents proliferation of FaDu cells by cell-cycle arrest and induction of apoptosis, via activating checkpoint kinase Chk1and Chk2 as well as caspases 8, 9 and 3. Meanwhile, we found that treatment with deltonin induced autophagy, which played a protective role against deltonin-induced apoptosis. Further studies revealed that deltonin activated autophagy by Akt-mTOR signaling. Additionally, xenograft model showed that administration of deltonin significantly inhibited tumor growth and prolonged survival of tumor bearing mice. Our studies suggested that deltonin might be a potential chemotherapeutic agent against HNSCC, which might contribute to clinical application and pharmacological study of deltonin in future anti-cancer research.

Effects of Wild Yam Root (Dioscorea villosa) Extract on the Gene Expression Profile of Triple-negative Breast Cancer Cells

“…These effects were concurrent with single gene up-regulation of the p21 gene CDKN1A, all of which are likely responsible for the observed cytostatic effects of WYE in a 6-day proliferation study. Our findings in this aspect support much of the existing literature for WYE and saponins from the Dioscorea species having the capacity to induce cell-cycle arrest (G 2 /M) across diverse cancer cell lines with observed downregulation of a similar list of cyclins and cell cycle regulatory transcripts (Cdc25C, Cdk1) (31)(32)(33)(34).…”

Alterations in the expression of DNA damage response-related molecules in potentially preneoplastic oral epithelial lesions

“…Recently, drugtriggered Chk2 activation has been suggested to possess anticancer effects in HNSCC. Hu et al 53 described that the antitumor role of curcumin is related to ataxia telangiectasia muted protein (ATM)/CHK2 activation and nuclear factor-κB inhibition, whereas Xie et al 54 showed that deltonin treatment activated Chk1 and Chk2 and the caspase pathway, leading to cell cycle arrest and induction of apoptosis.…”

Targeting microglial autophagic degradation of the NLRP3 inflammasome for identification of thonningianin A in Alzheimer’s disease

“…The proposed compounds with the corresponding retention time and the measured accurate mass in negative or positive ion mode were listed in Additional file 1: Table S1. Among them, the compounds with a retention time of 7.862-8.017 min were identified as ellagitannin flavonoids, including TA, TB, PHG, PGHG, and other unidentified compounds, which are the main components in PCP [35,49,60]. In addition, the representative TIC in negative ion mode and UV chromatogram at 254 nm for PCP leaf and steam parts were displayed in Additional file 1: Fig.…”

“…It is commonly used as a kind of health tea in the local region [4,51]. Emerging modern pharmacological studies show that PCP extract and its derived components exert potent anti-oxidation, anti-tumor, liver protection, and other effects [3,4,20,22,60,65,66,70]. The chemical analysis of PCP demonstrated that the main components are polyphenols, including rutin, quercetin, and ellagitannin flavonoids [17,21,49].…”