Demarcations of the mechanosensory projection zones in the raccoon thalamus, shown by cytochrome oxidase, acetylcholinesterase, and Nissl stains

Si, Wiener; Ji, Johnson; Em, Ostapoff

doi:10.1002/cne.902580404

Cited by 18 publications

(7 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intense CO activity is a feature of the VPL and VPM in other species (Wiener et al, 1987) as well as primates. The association of CO-dense staining patterns in the ventral posterior complex not only with the "lemniscal" (dorsal column and principal trigeminal) system of afferent fibers but also with parvalbumin immunoreactivity in these afferents and in thalamic relay cells, is established by the following observations.…”

Section: Discussionmentioning

confidence: 99%

Calbindin and parvalbumin cells in monkey VPL thalamic nucleus: distribution, laminar cortical projections, and relations to spinothalamic terminations

et al. 1992

View full text Add to dashboard Cite

The ventral posterior lateral nucleus (VPL) of the monkey thalamus was investigated by histochemical staining for cytochrome oxidase (CO) activity and by immunocytochemical staining for the calcium-binding proteins parvalbumin and 28 kDa calbindin. Anterograde and retrograde tracing experiments were used to correlate patterns of differential distribution of CO activity and of parvalbumin and calbindin cells with the terminations of spinothalamic tract fibers and with the types of cells projecting differentially to superficial and deeper layers of primary somatosensory cortex (SI). VPL is composed of CO-rich and CO- weak compartments. Cells are generally smaller in the CO-weak compartment. Parvalbumin-immunoreactive cells and parvalbumin- immunoreactive medial lemniscal fiber terminations are confined to the CO-rich compartment. Calbindin-immunoreactive cells are found in both the CO-rich and CO-weak compartments. The CO-weak compartment, containing only calbindin cells, forms isolated zones throughout VPL and expands as a cap covering the posterior surface of the ventral posterior medial nucleus (VPM). Spinothalamic tract terminations tend to be concentrated in the CO-weak compartment, especially in the posterior cap. Other CO-weak, parvalbumin-negative, calbindin-positive nuclei, including the posterior, ventral posterior inferior, and anterior pulvinar and the small-celled matrix of VPM are also associated with concentrations of spinothalamic and caudal trigeminothalamic terminations. Parvalbumin cells are consistently larger than calbindin cells and are retrogradely labeled only after injections of tracers in middle and deep layers of SI. The smaller calbindin cells are the only cells retrogradely labeled after placement of retrograde tracers that primarily involve layer I of SI. The compartmental organization of VPL is similar to but less rigid than that previously reported in VPM. VPL and VPM relay cells projecting to different layers of SI cortex can be distinguished by differential immunoreactivity for the two calcium-binding proteins. The small- celled, CO-weak, calbindin-positive zones of VPL and VPM appear to form part of a wider system of smaller thalamic neurons unconstrained by traditional nuclear boundaries that are preferentially the targets of spinothalamic and caudal trigeminal inputs, and that may have preferential access to layer I of SI.

show abstract

Section: Discussionmentioning

confidence: 99%

Calbindin and parvalbumin cells in monkey VPL thalamic nucleus: distribution, laminar cortical projections, and relations to spinothalamic terminations

et al. 1992

View full text Add to dashboard Cite

show abstract

“…The second compartment, the matrix domain, appears more diffuse in its organization, and the following paper (Rausell and Jones,199 1) shows it to be a likely route through which nonlemniscal information from the head, face, and mouth would be relayed to the SI. The 2 compartments of the VPM are clearly delineated in sections stained for CO, which has proven to be a successful marker for many compartments of physiological significance in both the somatic sensory and visual systems (Wong-Riley, 1979;Hubel, 1982, 1983;Horton, 1984;Jones et al, 1986a,b;Wiener et al, 1987). The rodlike organization in the VPM was first described in 1985; the relationships of the rods to the complete contralateral and partial ipsilateral maps of the head, face, and intraoral regions were then demonstrated, and individual rods through the full anteroposterior extent of the VPM were shown to contain the same representation (Jones, 1985;Jones et al, 1986a,b).…”

Section: Discussionmentioning

confidence: 99%

Histochemical and immunocytochemical compartments of the thalamic VPM nucleus in monkeys and their relationship to the representational map

Rausell¹,

Jones²

1991

J. Neurosci.

181

View full text Add to dashboard Cite

The ventral posteromedial nucleus (VPM) of the monkey thalamus was investigated with correlative anatomical and physiological techniques. On the basis of staining for cytochrome oxidase (CO), VPM is divided into a lightly stained, background matrix domain and an intensely stained rod domain. The latter consists of elongated rods of large, medium, and small cells, 500 microns wide on average and extending anteroposteriorly, many of them through the full extent of the nucleus. The matrix, consisting of small cells, penetrates between the rods and expands at the dorsomedial, ventrolateral, and posterior aspects of VPM. Multiunit mapping reveals that VPM contains a dorsally situated representation of the contralateral side of the head, face, eye, and interior of the mouth and a medially situated representation of the ipsilateral side of the lips and interior of the mouth, and that the same small region is represented in the same relative position through the full anteroposterior extent of the nucleus. Earlier work had shown that single CO rods contain the representation of the same portion of the periphery throughout their length. The present study suggests that rods in equivalent positions may represent the same portion of the periphery from animal to animal. The cells of the rod and matrix domains show different patterns of immunoreactivity. Virtually all of the large- and medium-sized rod cells are immunoreactive for the calcium-binding protein parvalbumin, and many are stained by the monoclonal antibody CAT 301. Small GABA-immunoreactive cells and terminal-like puncta are highly concentrated in the rods but are dispersed in the matrix. In the matrix, all non-GABA cells are small, immunoreactive for 28-kDa calbindin, and not stained by CAT 301. They appear to form part of a wider system of calbindin-positive cells that extends into adjacent nuclei. The CO rods are indicative of the modularity of the lemniscal component of the trigeminal part of the somatic sensory system at thalamic levels. Thalamocortical relay neurons in this compartment of VPM express a calcium-binding protein and a surface proteoglycan that distinguishes them from relay neurons in the matrix compartment of the nucleus. In the following paper (Rausell and Jones, 1991), the rod and matrix compartments are shown also to have different patterns of input and output connections.

show abstract

“…The septa, on the other hand, appear relatively free of these elements and instead may receive input from the spinal trigeminal nucleus (Williams et al, 1994). Barreloids thus resemble in many respects CO-defined subdivisions of monkey (Jones et al, 1986;Rausell and Jones, 1991) and raccoon thalamus (Wiener et al, 1987).…”

Section: Three-dimensional Architecture Of Barreloid Fieldmentioning

confidence: 98%

Barreloids in adult rat thalamus: Three‐dimensional architecture and relationship to somatosensory cortical barrels

Land

Buffer

Yaskosky

1995

J of Comparative Neurology

160

135

View full text Add to dashboard Cite

Histochemical staining for cytochrome oxidase (CO) and axonal transport of horseradish peroxidase (HRP) were used to investigate thalamocortical connections in the vibrissa-barrel system of adult rats. CO staining revealed that the medial division of the ventrobasal thalamic nucleus (VBm) consists of intensely stained rod-like configurations, containing thalamocortical projection neurons and intervening neuropil, separated by lighter-stained septa. CO-dark rods span the thickness of VBm, are arranged in a pattern of rows and arcs that resembles the distribution of vibrissae on the mystacial pad, and are similar to the cytoarchitectonic structures termed "barreloids" in the mouse thalamus. Based upon the dimensions of CO-dark structures and the numerical density of neurons in VBm we estimated that a barreloid in the rat may contain 250-300 neurons. HRP injections into lamina IV of the somatosensory cortex led to retrograde labeling of neurons within one or more barreloids. When injections were centered within the CO-dark hollows of cortical barrels about 95% of retrogradely labeled neurons were located in the barreloid that is isomorphic to the injected barrel; up to 5% of labeled neurons were located within a single adjacent barreloid. Barrel hollow injections that also included a barrel side yielded a larger proportion of labeled neurons in non-isomorphic barreloids. Interestingly, such extra-barreloid labeling was topologically consistent in that HRP-labeled neurons were distributed among barreloids that corresponded to cortical barrels nearest the injected barrel side. Injections into the septa between barrels similarly resulted in labeling within barreloids that corresponded to cortical barrels flanking the septal injection site. Following lamina IV injections the density of labeled neurons tended to be highest in the ventrolateral one-half to two-thirds of VBm. Retrograde labeling of neurons in the dorsomedial one-third to one-half of VBm was more often observed after HRP injections at the lamina V/VI border. Thus, barreloid neurons may be heterogeneous with respect to their laminar pattern of terminations within the somatosensory cortex. Some HRP injections in the cortex resulted in orthograde labeling of corticothalamic axons in the barreloids. When observed, labeled corticothalamic axons arborized principally within the barreloid isomorphic to the injected barrel column. Indeed, terminal labeling was densest in the vicinity of neurons retrogradely labeled by the same injection.

show abstract

Demarcations of the mechanosensory projection zones in the raccoon thalamus, shown by cytochrome oxidase, acetylcholinesterase, and Nissl stains

Cited by 18 publications

References 40 publications

Calbindin and parvalbumin cells in monkey VPL thalamic nucleus: distribution, laminar cortical projections, and relations to spinothalamic terminations

Calbindin and parvalbumin cells in monkey VPL thalamic nucleus: distribution, laminar cortical projections, and relations to spinothalamic terminations

Histochemical and immunocytochemical compartments of the thalamic VPM nucleus in monkeys and their relationship to the representational map

Barreloids in adult rat thalamus: Three‐dimensional architecture and relationship to somatosensory cortical barrels

Contact Info

Product

Resources

About