Demethylation of Polymethoxyflavones by Human Gut Bacterium, <i>Blautia</i> sp. MRG-PMF1

Burapan, Supawadee; Kim, Mihyang; Han, Jaehong

doi:10.1021/acs.jafc.7b00408

Cited by 52 publications

(60 citation statements)

References 34 publications

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“…Some Fusobacterium members are animal pathogens [63], but their consistent presence in wild bird guts [2,14,64,65], especially in herbivorous species [64][65][66], suggests a possible symbiotic role. Bacteria from the genus Blautia may facilitate the metabolism of plant secondary metabolites [67,68], consistent with an increase in the relative abundance of this genus in birds feeding on seeds. Ruminococcus bacteria (e.g., the R. gauvrauii and R. gnavus groups) that increased signi cantly with seed diet are within the family Lachnospiraceae and their possible functions are poorly resolved [69].…”

Section: P Major Gut Microbiomes Respond To Diet Changes But Not Asmentioning

confidence: 56%

Flexibility and resilience of Great tit (Parus major) gut microbiomes to changing diets

Bodawatta

Freiberga

Puzejova

et al. 2020

Preprint

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Background: Gut microbial communities play important roles in nutrient management and can change in response to host diets. The extent of this flexibility and the concomitant resilience is largely unknown in wild animals. To begin untangling the dynamics of avian-gut microbiome symbiosis associated with diet changes, we exposed Parus major (Great tits) fed with a standard diet (seeds and mealworms) to either a mixed (seeds, mealworms and fruits), a seed, or a mealworm diet for four weeks, and examined the flexibility of gut microbiomes to these compositionally different diets. To assess microbiome resilience (recovery potential), all individuals were subsequently reversed to a standard diet for another four weeks. Cloacal microbiomes were collected weekly and characterised through sequencing the v4 region of the 16S rRNA gene using Illumina MiSeq. Results: Initial microbiomes changed significantly with the diet manipulation but the communities did not differ significantly between the three diet groups (mixed, seed and mealworm), despite multiple diet-specific changes of specific bacterial genera. Reverting birds to the standard diet led to only a partial recovery in gut community compositions. The majority of the bacterial taxa that increased significantly during diet manipulation decreased in relative abundances after reversion to the standard diet; however, bacterial taxa that decreased during the manipulation rarely increased after diet reversal.Conclusions: The gut microbial response and partial resilience to dietary changes support that gut bacterial communities of P. major play a role in accommodating dietary changes experienced by wild avian hosts. This may be a contributing factor to the relaxed association between microbiome composition and bird phylogeny. Our findings further imply that interpretations of wild bird gut microbiome analyses from single-time point sampling, especially for omnivorous species or species that have changing seasonal diets, should be done with caution. The partial community recovery implies that ecologically relevant diet changes (e.g., seasonality and migration) open up gut niches that may be filled by previously abundant microbes or replaced by different symbiont lineages, which has important implications for the integrity and specificity of long-term avian-symbiont associations.

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Section: P Major Gut Microbiomes Respond To Diet Changes But Not Asmentioning

confidence: 56%

Flexibility and resilience of Great tit (Parus major) gut microbiomes to changing diets

Bodawatta

Freiberga

Puzejova

et al. 2020

Preprint

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“…An in vitro study showed that Blautia sp. MRG-PMF1 bio-transformed polymethoxyflavones (PMFs) in chrysin, apigenin, galangin, kaempferol, luteolin, and quercetin [60]. This class of flavonoids is involved in many biological functions, among which it exerts important role in anticancer, anti-inflammation, antiallergic, antimutagenicity, and neuroprotection activities.…”

Section: Lachnospiraceae Metabolismmentioning

confidence: 99%

The Controversial Role of Human Gut Lachnospiraceae

et al. 2020

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The complex polymicrobial composition of human gut microbiota plays a key role in health and disease. Lachnospiraceae belong to the core of gut microbiota, colonizing the intestinal lumen from birth and increasing, in terms of species richness and their relative abundances during the host’s life. Although, members of Lachnospiraceae are among the main producers of short-chain fatty acids, different taxa of Lachnospiraceae are also associated with different intra- and extraintestinal diseases. Their impact on the host physiology is often inconsistent across different studies. Here, we discuss changes in Lachnospiraceae abundances according to health and disease. With the aim of harnessing Lachnospiraceae to promote human health, we also analyze how nutrients from the host diet can influence their growth and how their metabolites can, in turn, influence host physiology.

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“…In fact, in rat liver microsomes, tangeretin is metabolized to 4'-hydroxy-5,6,7,8-tetramethoxyflavone and 3',4'-dihydroxy-5,6,7,8-tetramethoxyflavone [44]. Even at intestinal levels, the metabolism of PMF leads to the formation of demethylated metabolites by intestinal bacteria [45,46]. After 4 h by oral administration, only 24% of tangeretin was detected in the organs and digestive tract, indicating that approximately 76% of flavone is absorbed or retained in the lumen of the digestive tract as metabolites, such as demethylated derivatives [44].…”

Section: Absorption Uptake and Pharmacokineticsmentioning

confidence: 99%

“…At systemic circulation level, diosmetin is enzymatically esterified to its relevant metabolite, 3-O-glucuronide, which is further esterified into 3,7-di-O-glucuronide [47,48]. Experimental evidence has shown that diosmin has the ability to inhibit P-glycoprotein [45] and several cytochrome P450 (CYP) enzyme isoforms (CYP1A1, CYP1A2, CYP2B1, CYP2C8, CYP2C9, CYP2D6, CYP2E1, CYP3A4, and CYP3A5). Specifically, the inhibition of drug-biotransformation CYP 450 enzymes [49] influences metabolic and pharmacokinetic properties of themselves and other simultaneously administered drugs [50].…”

Section: Absorption Uptake and Pharmacokineticsmentioning

confidence: 99%

Citrus Flavones: An Update on Sources, Biological Functions, and Health Promoting Properties

Barreca

Mandalari

Calderaro

et al. 2020

Plants

103

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Citrus spp. are among the most widespread plants cultivated worldwide and every year millions of tons of fruit, juices, or processed compounds are produced and consumed, representing one of the main sources of nutrients in human diet. Among these, the flavonoids play a key role in providing a wide range of health beneficial effects. Apigenin, diosmetin, luteolin, acacetin, chrysoeriol, and their respective glycosides, that occur in concentrations up to 60 mg/L, are the most common flavones found in Citrus fruits and juices. The unique characteristics of their basic skeleton and the nature and position of the substituents have attracted and stimulated vigorous investigations as a consequence of an enormous biological potential, that manifests itself as (among other properties) antioxidant, anti-inflammatory, antiviral, antimicrobial, and anticancer activities. This review analyzes the biochemical, pharmacological, and biological properties of Citrus flavones, emphasizing their occurrence in Citrus spp. fruits and juices, on their bioavailability, and their ability to modulate signal cascades and key metabolic enzymes both in vitro and in vivo. Electronic databases including PubMed, Scopus, Web of Science, and SciFinder were used to investigate recent published articles on Citrus spp. in terms of components and bioactivity potentials.

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Demethylation of Polymethoxyflavones by Human Gut Bacterium, Blautia sp. MRG-PMF1

Cited by 52 publications

References 34 publications

Flexibility and resilience of Great tit (Parus major) gut microbiomes to changing diets

Flexibility and resilience of Great tit (Parus major) gut microbiomes to changing diets

The Controversial Role of Human Gut Lachnospiraceae

Citrus Flavones: An Update on Sources, Biological Functions, and Health Promoting Properties

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