Demineralized bone matrix and calcium-phosphate cement in bone regeneration in rats

Silva, Luiz Carlos Ferreira da; Porto, Gabriela Granja; Andrade, Emanuel Sávio de Souza; Filho, José Rodrigues Laureano

doi:10.1590/s0102-865020180040000007

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…Our results are similar to those in other studies in which DBM was implanted together with calcium phosphate cement, where the calcium phosphate cement acted by inhibiting osteogenesis when associated with a DBM [ 11 ].…”

Section: Discussionsupporting

confidence: 91%

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Demineralized Bone Matrix Coating Si-Ca-P Ceramic Does Not Improve the Osseointegration of the Scaffold

et al. 2018

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The aim of this study was to manufacture and evaluate the effect of a biphasic calcium silicophosphate (CSP) scaffold ceramic, coated with a natural demineralized bone matrix (DBM), to evaluate the efficiency of this novel ceramic material in bone regeneration. The DBM-coated CSP ceramic was made by coating a CSP scaffold with gel DBM, produced by the partial sintering of different-sized porous granules. These scaffolds were used to reconstruct defects in rabbit tibiae, where CSP scaffolds acted as the control material. Micro-CT and histological analyses were performed to evaluate new bone formation at 1, 3, and 5 months post-surgery. The present research results showed a correlation among the data obtained by micro-CT and the histomorphological results, the gradual disintegration of the biomaterial, and the presence of free scaffold fragments dispersed inside the medullary cavity occupied by hematopoietic bone marrow over the 5-month study period. No difference was found between the DBM-coated and uncoated implants. The new bone tissue inside the implants increased with implantation time. Slightly less new bone formation was observed in the DBM-coated samples, but it was not statistically significant. Both the DBM-coated and the CSP scaffolds gave excellent bone tissue responses and good osteoconductivity.

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Section: Discussionsupporting

confidence: 91%

“…DBM products are available in several commercial presentations, such as morselized material, powder, putty pastes, chips, porous foams, gel, etc. [ 10 , 11 , 12 ]. Nevertheless, a high osteogenic capacity rate of bone is lost while it is processed [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Demineralized Bone Matrix Coating Si-Ca-P Ceramic Does Not Improve the Osseointegration of the Scaffold

et al. 2018

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“…In this study, we compared osteogenic effects of TBC and DBM on femoral condyle and radial defects. The results indicated that the repair with xenograft bone scaffolds of 8 weeks were needed and the repair results were better than those of 4 weeks whatever the type of defects, which was in line with previous studies [ 19 , 34 ].…”

Section: Discussionsupporting

confidence: 91%

Comparison of osteogenesis of bovine bone xenografts between true bone ceramics and decalcified bone matrix

Guo²,

Han³

et al. 2022

J Mater Sci: Mater Med

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Xenograft bone scaffolds have certain advantages such as mechanical strength, osteoinductive properties, sufficient source and safety. This study aimed to compare osteogenesis of the two main bovine bone xenografts namely true bone ceramics (TBC) and decalcified bone matrix (DBM), and TBC or DBM combined with bone morphogenetic protein (BMP)-2 (TBC&BMP-2 and DBM&BMP-2). The characteristics of TBC and DBM were investigated by observing the appearance and scanning electron microscopic images, examining mechanical strength, evaluating cytotoxicity and detecting BMP-2 release after being combined with BMP-2 in vitro. The femoral condyle defect and radial defect models were successively established to evaluate the performance of the proposed scaffolds in repairing cortical and cancellous bone defects. General observation, hematoxylin and eosin (HE) staining, mirco-CT scanning, calcein double labeling, X-ray film observation, three-point bending test in vivo were then performed. It indicated that the repair with xenograft bone scaffolds of 8 weeks were needed and the repair results were better than those of 4 weeks whatever the type of defects. To femoral condyle defect, TBC and TBC&BMP-2 were better than DBM and DBM&BMP-2, and TBC&BMP-2 was better than TBC alone; to radial defect, DBM and DBM&BMP-2 were better than TBC and TBC&BMP-2, and DBM&BMP-2 was better than DBM alone. This study has shown that TBC and DBM xenograft scaffolds can be more suitable for the repair of cancellous bone and cortical bone defects for 8 weeks in rats, respectively. We also have exhibited the use of BMP-2 in combination with DBM or TBC provides the possibility to treat bone defects more effectively. We thus believe that we probably need to select the more suitable scaffold according to bone defect types, and both TBC and DBM are promising xenograft materials for bone tissue engineering and regenerative medicine.

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“…Cyanoacrylate has yet to be tested on bone repair outside of maxillofacial surgeries [7]. Demineralized bone matrix has shown promise in filling in defects in bone [8]. There has yet to be an effective glue combination with demineralized bone matrix without severely compromising the strength of the bonding adhesive [8].…”

Section: Discussionmentioning

confidence: 99%

“…Demineralized bone matrix has shown promise in filling in defects in bone [8]. There has yet to be an effective glue combination with demineralized bone matrix without severely compromising the strength of the bonding adhesive [8]. This combination of cyanoacrylate glue and demineralized bone matrix could provide the quick and simple repair needed for comminuted fractures.…”

Section: Discussionmentioning

confidence: 99%

A novel repair for comminuted fractures in metacarpals: Combining cyanoacrylate with demineralized bone matrix

Stephanides¹,

Farinas²,

Johnson³

et al. 2018

Surg Rehabil

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Background: Comminuted fractures of the metacarpals are both extremely painful and difficult for surgeons to repair. A strong, fast acting repair must be made to realign minute fragments of bone properly. Cyanoacrylate and demineralized bone matrix (DBM) have proven effective individually for certain repairs, but the efficacy of a combined treatment has not been confirmed. The purpose of this study is to test the strength of the repair when using a combined treatment of cyanoacrylate mixed with DBM in comminuted fractures.Methods: Sixteen metacarpals (2nd-5th) were collected from cadavers and fractured into three pieces at 30-degree angles to simulate a comminuted fracture. Three treatment groups were assessed: repair with cyanoacrylate glue (n=4), repair with cyanoacrylate and DBM combined (n=4), and repair using titanium plates and screws (n=8). Bones were pulled apart until repair failure with an Instron 5542 machine and the load at break (N) was recorded for each sample. IBM SPSS Statistics 24 was used to perform all statistical analysis. Independent sample t-tests were performed to compare group sample means. P-values < 0.05 were considered significant. Results:The titanium plate with screws repair demonstrated a significantly higher load at break across all metacarpals (avg=91.745 ± 12.575). There was no significant difference between the load at break for the cyanoacrylate glue (avg=39.855 ± 8.105) and the cyanoacrylate glue mixed with DBM groups (avg=28.664 ± 8.375). The 4th metacarpal produced the lowest load at break for the titanium plate with screws repair and the cyanoacrylate repair while the 5th metacarpal presented the lowest load at break for the cyanoacrylate mixed with DBM repair. Conclusions:The addition of DBM to cyanoacrylate does not significantly compromise the adhesive strength of cyanoacrylate. Titanium plates offer the strongest repair of the three groups. When bone fragments are too small to attach plates, the cyanoacrylate and DBM mixture creates a putty allowing easy realignment of the fragments while maintaining the strength of cyanoacrylate. This study was not conducive to examining DBM's osteoinductive nor osteoconductive properties since all tests were done ex vivo. Further research should examine repairs over time using cyanoacrylate mixed with DBM in vivo.

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Demineralized bone matrix and calcium-phosphate cement in bone regeneration in rats

Cited by 6 publications

References 21 publications

Demineralized Bone Matrix Coating Si-Ca-P Ceramic Does Not Improve the Osseointegration of the Scaffold

Demineralized Bone Matrix Coating Si-Ca-P Ceramic Does Not Improve the Osseointegration of the Scaffold

Comparison of osteogenesis of bovine bone xenografts between true bone ceramics and decalcified bone matrix

A novel repair for comminuted fractures in metacarpals: Combining cyanoacrylate with demineralized bone matrix

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