1985
DOI: 10.1007/bf02554872
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Demonstration and characterization of 1,25-dihydroxyvitamin D3 receptors in basal cells of epidermis of neonatal and adult mice

Abstract: Nuclear and cytosolic receptors for 1,25-dihydroxycholecalciferol [1,25(OH)2D3] were demonstrated in the epidermis of neonatal and adult mice. The macromolecular binding protein sedimented at 3.5 S (sucrose density gradient) and was distinct from the 6.0 S binding protein for 25-hydroxycholecalciferol [25(OH)D3]. Analysis at different ionic strengths suggested the presence of unoccupied nuclear receptors. Digestion with proteases or nucleases, respectively, and inactivation with alkylating agents demonstrated … Show more

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Cited by 33 publications
(18 citation statements)
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“…UVB-induced membrane damage to epidermal keratinocytes may also increase the outflow of newly synthesized calcitriol(92). The receptor for calcitriol and the production of 1,25(OH)2D vary with the differentiation in a manner suggesting feedback regulation, and both are reduced in the later stages of differentiation (93). 1,25(OH)2D increases involucrin, transglutaminase activity, and cornified envelope formation in preconfluent keratinocytes (94).…”
Section: The Effects Of Phototherapy On Vitamin D Status In Patients mentioning
confidence: 99%
“…UVB-induced membrane damage to epidermal keratinocytes may also increase the outflow of newly synthesized calcitriol(92). The receptor for calcitriol and the production of 1,25(OH)2D vary with the differentiation in a manner suggesting feedback regulation, and both are reduced in the later stages of differentiation (93). 1,25(OH)2D increases involucrin, transglutaminase activity, and cornified envelope formation in preconfluent keratinocytes (94).…”
Section: The Effects Of Phototherapy On Vitamin D Status In Patients mentioning
confidence: 99%
“…Cell suspensions (1.5 X I07 cells/ ml) obtained by trypsinization were homogenized in TED buffer, i.e., 10 mM Tris HCI, 1.5 mM EDTA, 2 mM DTT, 10 mM sodium molybdate, pH 7.4. Crude fractions and nuclear extracts were prepared as described elsewhere (7,10) and used for sucrose density-gradient analysis, binding studies, or DNA affinity chromatography.…”
Section: Assay Of Vdr Activitymentioning
confidence: 99%
“…Cell extracts were prepared as described elsewhere (7,10,20). In brief, cell suspensions (1.5 X 107 cells/ml) were homogenized in 0.4 M KTED buffer (TED buffer supplemented with 0.4 M KC1).…”
Section: Assay Of Vdr Activitymentioning
confidence: 99%
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