Demonstration of a Cytoplasmic Receptor Protein for Oestrogen in the Canine Prostate Gland

Chaisiri, N.; Valotaire, Yves; Evans, Bronwen Alice James; Pierrepoint, C. G.

doi:10.1677/joe.0.0780131

Cited by 29 publications

(12 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mechanism of this interaction, however, is unclear. Moore et al (13) Insight into the mechanism of action of estrogen in the canine prostate has been aided by the identification of a specific estrogen receptor (10). In this study, we have demonistrated that treatmiient of castrates with estradiol results in an increase in both cytosolic and nuclear estrogen-receptor content and in the appearance of a cytosolic progesterone receptor.…”

mentioning

confidence: 57%

“…These molecules bind with high affinity to steroid hormones within the cytosol and are translocated to the nucleus where the cellular response is initiated. Both androgen and estrogen receptors have l)een identified and characterized within the canine prostate (9,10 Ill.), and the radioactivity counted with an efficiency of 34%. The data were analyzed by the method of Scatchard (14) [3H]estradiol to yield final labeled ligand concentrations of 0.15-10 nM.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Androgen- and Estrogen-Receptor Content in Spontaneous and Experimentally Induced Canine Prostatic Hyperplasia

Trachtenberg¹,

Hicks²,

Walsh³

1980

J. Clin. Invest.

138

View full text Add to dashboard Cite

A B S T R A C T To gain insight into the mechanism by which steroidal hormones influence the development of canine prostatic hyperplasia, nuclear and cytosolic androgen-and estrogen-receptor content, as measured under exchange conditions by the binding of[3H]R1881 (methyltrienolone) and [3H]estradiol, respectively, were quantitated in the prostates of purebred beagles ofknown age. In young dogs with spontaneously arising and experimentally induced (androstanediol plus estradiol treatment) prostatic hyperplasia, nuclear, but not cytosolic, prostatic androgen-receptor content was significantly greater than that determined in the normal prostates of age-matched dogs (3,452+222 and 4,035 ±274 fmol/mg DNA vs. 2,096±364 fmol/mg DNA, respectively). No differences were observed between the androgen-receptor content of the normal prostates of young dogs and the hyperplastic prostates of old dogs. The cytosolic and nuclear estrogen-receptor content of spontaneously arising prostatic hyperplasia in both young and old animals was similar to that found in normal prostates. The administration of estradiol plus androstanediol to castrate dogs significantly increased the prostatic nuclear androgen-receptor content over that found in dogs treated only with androstanediol. This estradiol-associated increase in nuclear androgenreceptor content was accompanied by the development of benign prostatic hyperplasia.Estradiol treatment of castrate dogs resulted in an increase in prostatic nuclear estrogen-receptor content, in the appearance of a putative prostatic cytosolic progesterone receptor, and in an alteration of the epithelium of the prostate to one characterized by squamous metaplasia. Treatment of castrate dogs with both estradiol and androstanediol resulted in a reducDr. Trachtenberg is a Fellow of the Medical Research Council of Canada.

show abstract

mentioning

confidence: 57%

mentioning

confidence: 99%

Androgen- and Estrogen-Receptor Content in Spontaneous and Experimentally Induced Canine Prostatic Hyperplasia

Trachtenberg¹,

Hicks²,

Walsh³

1980

J. Clin. Invest.

138

View full text Add to dashboard Cite

show abstract

“…Using tissue from patients with benign hyperplasia of the prostate, evidence for the presence of oestradiol and its close relationship to dihydrotestosterone and 5cc-androstane3a,17ß-diol has recently been presented by Ghanadian & Puah (1981). In addition to the existence of oestradiol in the prostate, the presence of cytoplasmic oestrogen receptors has been reported in the prostate of the rat (Armstrong & Bashirelahi, 1978), dog (Chaisiri, Volotaire, Brownen, Evans & Pierrepoint, 1978), baboon (Karr, Sufrin, Kirdani, Murphy & Sandberg, 1978) and normal man (Karr, Wajsman, Madajewicz, Kirdani, Murphy & Sandberg, 1979). However, data with regard to the presence of this receptor in the benign hypertrophied human prostate (BPH) has been controversial.…”

Section: Introductionmentioning

confidence: 99%

Characterization and measurement of cytoplasmic and nuclear oestradiol-17β-receptor proteins in benign hypertrophied human prostate

Auf¹,

Ghanadian²

1982

Journal of Endocrinology

View full text Add to dashboard Cite

The demonstration and partial characterization of a high-affinity saturable binding component for oestradiol-17 beta in the cytosol and nuclear extract of the benign hypertrophied human prostate is reported. This binding component was found to precipitate with protamine sulphate and to exhibit marked specificity for oestradiol and diethylstilboestrol (DES) but not dihydrotestosterone (DHT) or progesterone. Analysis of oestradiol-labelled cytosol on low ionic strength glycerol gradients revealed a binding component with a sedimentation coefficient of 4S which was inhibited with DES but not DHT and destroyed either by preheating labelled cytosol at 45 degrees C for 30 min or by treatment with the sulphydryl blocking agent, parachloromercureobenzoate. The oestradiol-binding complex precipitated by ammonium sulphate was found to have an isoelectric point (pI) of 6.3 as analysed on polyacrylamide-gel plates. The oestradiol-binding component in the nuclear extract also exhibited similar steroid specificity and sedimented with a coefficient of 2.8S on high ionic strength glycerol gradients. Isoelectric focusing of the heparin-extracted nuclear oestradiol-binding complex revealed a pI of 6.0. The dissociation constants (Kd) and the concentrations of the cytoplasmic and nuclear oestradiol-binding sites in 19 samples of benign hypertrophied prostates were estimated by Scatchard plot analyses. The mean Kd and concentration of binding sites in the cytosol were 2.9 +/- 2.6 (S.D.) nmol/l and 11.7 +/- 8.4 fmol/mg protein respectively. The corresponding values for nuclear extract were 4.1 +/- 7.5 nmol/l and 347.1 +/- 415.5 fmol/mg DNA respectively. There was no significant difference between the mean concentration of oestradiol-binding sites in the two cellular fractions.

show abstract

“…The possible role of E2 in the development of canine prostatic hyperplasia is supported by the observation of an increased endogenous concen tration of E2 in prostatic tissue (28). Further more, cytoplasmatic receptor protein for E2 in canine prostates has been demonstrated previ ously (10).…”

Section: Discussionmentioning

confidence: 98%

Effects of Cyproterone Acetate on Experimentally Induced Canine Prostatic Hyperplasia

Tunn¹,

Senge²,

Schenck³

et al. 1980

Urol Int

View full text Add to dashboard Cite

The effect of 3α-androstanediol (3α-diol), 17β-estradiol (E₂) and cyproterone acetate (CA) on prostates in castrated beagle dogs were investigated by histologjcal and histochemical examinations. A significant increase of prostatic weight occurred after 6 months’ treatment with 3α-diol alone and in combination with E₂. Histologically and histochemically, two different types of prostatic enlargement were observed: first, administration of 3α-diol resulted in diffuse glandular hyperplasia with replacement of functional activity monitored by strongly positive reactions for acid phosphatase, aminopeptidase and zinc. Second, 3α-diol plus E₂ produced stratified squamous metaplasia with cystic lumina and loss of the typical morphological structure. These glands showed negative reactions for acid phosphatase, aminopeptidase and zinc. In both types of prostatic hyperplasia CA abolished epithelial or metaplastic proliferation and induced atrophy of glandular epithelium. In estrogenized dogs activation of the fibromuscular stroma was obvious. CA prevented prostatic hyperplasia by atrophying epithelial effects.

show abstract

Demonstration of a Cytoplasmic Receptor Protein for Oestrogen in the Canine Prostate Gland

Cited by 29 publications

References 15 publications

Androgen- and Estrogen-Receptor Content in Spontaneous and Experimentally Induced Canine Prostatic Hyperplasia

Androgen- and Estrogen-Receptor Content in Spontaneous and Experimentally Induced Canine Prostatic Hyperplasia

Characterization and measurement of cytoplasmic and nuclear oestradiol-17β-receptor proteins in benign hypertrophied human prostate

Effects of Cyproterone Acetate on Experimentally Induced Canine Prostatic Hyperplasia

Contact Info

Product

Resources

About