Demonstration of a cytotoxin from Campylobacter jejuni.

Yeen, Wong Phooi; Puthucheary, S. D.; Pang., Thong

doi:10.1136/jcp.36.11.1237

Cited by 54 publications

(21 citation statements)

References 14 publications

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“…26 Thus the role of enterotoxin in disease remains uncertain. Several workers have reported C. jejuni cytotoxins active against various cell 27 All the colitis strains in our collection, and 18 of 23 isolates from noninflammatory diarrhoea, produced activity that was lethal to cultured CHO cells. This is a statistically significant difference, and may suggest a role in disease.…”

Section: Discussionmentioning

confidence: 99%

Differentiated Caco-2 cells as a model for enteric invasion by Campylobacter jejuni and C. coli

Everest

Goossens

Butzler

et al. 1992

Journal of Medical Microbiology

154

118

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Summary.A collection of 44 Campylobacter isolates (37 C. jejuni and seven C. coli) from children with colitis (21 strains) or watery diarrhoea (23 strains) was analysed for toxin production, association with HeLa cells, and invasion of differentiated Caco-2 cell cultures. There was no obvious association of clinical symptoms with species, biotype or enterotoxin production. All colitis strains and most of the isolates from watery diarrhoea were cytotoxic for Chinese hamster ovary cells. Measurements of bacterial association indices with HeLa cells varied with time, and were considered to be unreliable for discriminating between isolates from the two diagnostic groups. Statistically significant differences were observed between the two groups (all colitis strains and 65% of strains from non-inflammatory diarrhoea) with respect to invasion of both HeLa and Caco-2 cell monolayers. However, among the strains from non-inflammatory diarrhoea that did invade, numbers of internalised bacteria were similar to the range observed for colitis strains. Of the colitis strains, 86 % were able to transcytose through polarised Caco-2 monolayers grown on filters, compared with 48% of isolates from non-inflammatory disease. We propose the use of Caco-2 cells as a model for studying invasion of intestinal epithelia by C. jejuni and C. coli.

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Section: Discussionmentioning

confidence: 99%

Differentiated Caco-2 cells as a model for enteric invasion by Campylobacter jejuni and C. coli

Everest

Goossens

Butzler

et al. 1992

Journal of Medical Microbiology

154

118

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“…A subsequent report indicated the presence of a Shiga-like cytotoxin from C. jejuni which could be neutralised with monoclonal antibodies directed against the B subunit of the mature Shigatoxin; however, these workers also detected a cytotoxin which could not be neutralised by the same monoclonal antibody [3 11. In addition, Guerrant and colleagues [12], unlike Yeen et al [30], found cytotoxic activity in Fig.4. Double staining of native-PAGE (lanes 1 and 2) and SDS-PAGE (lanes 3 and 4) gels with periodic acid-Schiff (PAS) and Coomassie Blue.…”

Section: Discussionmentioning

confidence: 99%

“…A cytotoxin from strains of C. jejuni that was heatlabile, trypsin-sensitive and induced characteristic rounding of HEp-2, HeLa and MRC-5 cells was first documented by Yeen et al [30]. Guerrant et al…”

Section: Discussionmentioning

confidence: 99%

Identification and characterisation of a cytotoxic porin-lipopolysaccharide complex from Campylobacter jejuni

Bacon¹,

Johnson

Rodgers³

1999

Journal of Medical Microbiology

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A clinical isolate of Campylobacter jejuni, previously found to produce a toxin active in cell culture assays, was used for identification and characterisation of a cytotoxic porinlipopolysaccharide (LPS) complex. This cytotoxic complex was isolated by highperformance liquid chromatography of crude concentrated culture supernate and DEAE-anion exchange chromatography. The complex had a toxic activity of 20.1 tissue culture dose50 (TCD50)lpg of protein for HEp-2 cells, 7.49 TCD5O/pg of protein for HeLa cells and 1.87 TCD5Olpg of protein for Chinese hamster ovary cells. Analysis by SDS-PAGE revealed a single protein band of 45 kDa and a high mol. wt carbohydrate moiety. The complex gave a positive result in the Limulus amoebocyte lysate test, indicating that the co-purifying carbohydrate was LPS, and had specificity for the lectins Galanthus nivalis agglutinin, Maackia amurensis agglutinin and Datura stramonium agglutinin. The cytotoxic activity associated with the complex was heatlabile at 70°C, resistant to inactivation with trypsin and retained activity after treatment with sodium metaperiodate and the glycosidases neuraminidase and N-glycosidase F. Sequencing of the N-terminus of the protein component of the complex revealed 97% homology with the major outer-membrane porin protein from C. jejuni. The cytotoxic activity of the complex was neutralised by a polyclonal, homologous antiserum, which reacted on Western blot with the 45-kDa protein, but not by polyclonal antisera raised against a number of other bacterial toxins.

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“…In 1983, a cytotoxin was discovered in C. jejuni that was mainly associated with isolates that induced acute gastroenteritis and/or bacteremia [102]. Further, a cytopathic effect was observed when Hela cells were treated with filtrates from 48-h C. jejuni cultures.…”

Section: Cytotoxinmentioning

confidence: 99%

“…Further, a cytopathic effect was observed when Hela cells were treated with filtrates from 48-h C. jejuni cultures. This effect could not be reproduced when the filtrates were heated or treated with trypsin, indicating that the cytotoxin was most probably a protein [102]. Further, in a RITARD model of infection, rabbits developed bacteremia and severe watery mucuscontaining diarrhoea (some even died) when exposed to C. jejuni cytotoxin-positive strains, whereas rabbits inoculated with cytotoxin-negative C. jejuni isolates developed less severe diarrhoea and none died [103].…”

Section: Cytotoxinmentioning

confidence: 99%